| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,250 | T→C | 33.3% | T236T (ACT→ACC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,250 | 0 | T | C | 33.3% | 78.5 / 61.7 | 75 | T236T (ACT→ACC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (28/22); new base C (11/14); total (39/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCA > minE/832180‑832318 | cTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACt < 1:163062/71‑1 (MQ=255) cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACg < 1:1722190/71‑1 (MQ=255) tAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGt < 1:3375919/70‑1 (MQ=255) tAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGt < 1:3382378/70‑1 (MQ=255) aaGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTa < 1:1006427/70‑1 (MQ=255) aaGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGt < 1:4166571/69‑1 (MQ=255) tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGaa > 1:1655704/1‑71 (MQ=255) tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGa < 1:3303868/70‑1 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:999977/69‑1 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:2123804/69‑1 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:3042141/1‑71 (MQ=255) tACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:1807426/1‑70 (MQ=255) tACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATc > 1:1283628/1‑71 (MQ=255) tACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATc > 1:1396551/1‑71 (MQ=255) aCGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:4469270/1‑69 (MQ=255) cGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATc < 1:5684819/69‑1 (MQ=255) cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt < 1:90272/68‑1 (MQ=255) cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATct > 1:4196601/1‑70 (MQ=255) cTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATctgctg < 1:1065204/71‑1 (MQ=255) tGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCgg < 1:1009180/61‑1 (MQ=255) ggTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGAc > 1:5140846/1‑71 (MQ=255) ttGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGaa < 1:2171945/57‑1 (MQ=255) ttGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGAc > 1:1859496/2‑67 (MQ=255) cTGCGTCTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACt < 1:1194552/68‑1 (MQ=255) gCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTa > 1:180735/1‑70 (MQ=255) gCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTa > 1:3097322/1‑70 (MQ=255) gCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGa > 1:3131241/1‑46 (MQ=255) gCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAAt > 1:5092699/1‑57 (MQ=255) gCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAAt > 1:3352922/1‑57 (MQ=255) gCGACATGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTa > 1:2420630/1‑70 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTg > 1:2286052/1‑66 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:1238824/70‑1 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:3566441/70‑1 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACa < 1:945171/71‑1 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACa < 1:4997379/71‑1 (MQ=255) gACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACaa < 1:3450789/71‑1 (MQ=255) aCTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAAc > 1:3771725/1‑71 (MQ=255) ttGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTc > 1:418955/1‑71 (MQ=255) cctcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAg > 1:2857378/1‑71 (MQ=255) ctcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTa > 1:4872348/1‑41 (MQ=255) ctcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTa > 1:4903174/1‑41 (MQ=255) ctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTa > 1:3282601/1‑70 (MQ=255) ctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTa > 1:5526511/1‑70 (MQ=255) ctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGATa > 1:2386521/1‑70 (MQ=255) cGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcat > 1:5357351/1‑70 (MQ=255) tcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGaa > 1:5625362/1‑37 (MQ=255) tcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGaa > 1:267698/1‑37 (MQ=255) tcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:3440159/36‑1 (MQ=255) tcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:5655540/36‑1 (MQ=255) tcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCg > 1:1374314/1‑71 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACatcatc < 1:1900274/68‑1 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACatcatc < 1:3833639/68‑1 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACatcatc < 1:4710229/68‑1 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGcc < 1:5186408/71‑1 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGcc < 1:3036599/71‑1 (MQ=255) atgaCCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTg > 1:1306513/4‑71 (MQ=255) gaCCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTggg > 1:3806285/2‑71 (MQ=255) gaCCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTga < 1:3316019/69‑2 (MQ=255) cTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGt > 1:3091976/1‑71 (MQ=255) cTGCGGCGAGCAAACTGACGTACCGGAATCTGCTGACTgg < 1:724092/40‑1 (MQ=255) tGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGc > 1:5623445/1‑62 (MQ=255) ggggCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGc > 1:5555730/1‑50 (MQ=255) ggggCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTata > 1:1116689/1‑40 (MQ=38) gggCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCa > 1:2219594/1‑70 (MQ=255) gggCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTct > 1:1850287/1‑69 (MQ=255) gggCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTct < 1:3094541/70‑2 (MQ=255) gCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTg < 1:1357902/61‑1 (MQ=255) gAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGt < 1:4493414/71‑1 (MQ=255) aGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCg < 1:998085/54‑1 (MQ=38) gCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGc < 1:383673/71‑1 (MQ=255) gCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCg < 1:463908/53‑1 (MQ=38) gCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGtac < 1:120337/71‑3 (MQ=255) aaCTGACGTACTGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCg > 1:2519185/1‑70 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCg > 1:3401781/1‑70 (MQ=255) aCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCa < 1:2961473/71‑1 (MQ=255) cTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCt > 1:520417/1‑51 (MQ=255) tGACGTACCGGAATCTGCTGACTGGTACAACTCCa < 1:5598279/35‑1 (MQ=255) tGACGTACCGGAATCTGCTGACTGGTACAACTCCa < 1:5091767/35‑1 (MQ=255) tGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTgggg < 1:3502929/54‑1 (MQ=255) tGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACg > 1:3295964/1‑62 (MQ=255) tGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACg > 1:170113/1‑62 (MQ=255) | CTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCA > minE/832180‑832318 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |