| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,277 | C→T | 39.2% | Y245Y (TAC→TAT) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,277 | 0 | C | T | 39.2% | 75.3 / 103.0 | 102 | Y245Y (TAC→TAT) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (33/29); new base T (20/20); total (53/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
CGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTT > minE/832208‑832345 | cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:1238824/70‑1 (MQ=255)cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:3566441/70‑1 (MQ=255)cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACa < 1:945171/71‑1 (MQ=255)cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACa < 1:4997379/71‑1 (MQ=255) aCTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAAc > 1:3771725/1‑71 (MQ=255) ttGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTc > 1:418955/1‑71 (MQ=255) cctcctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAg > 1:2857378/1‑71 (MQ=255) ctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTa > 1:3282601/1‑70 (MQ=255) ctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTa > 1:5526511/1‑70 (MQ=255) ctGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGATa > 1:2386521/1‑70 (MQ=255) cGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTAcat > 1:5357351/1‑70 (MQ=255) tcCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCg > 1:1374314/1‑71 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACatcatc < 1:4710229/68‑1 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACatcatc < 1:1900274/68‑1 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACatcatc < 1:3833639/68‑1 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGcc < 1:5186408/71‑1 (MQ=255) cGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGcc < 1:3036599/71‑1 (MQ=255) atgaCCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTg > 1:1306513/4‑71 (MQ=255) gaCCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTggg > 1:3806285/2‑71 (MQ=255) gaCCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTga < 1:3316019/69‑2 (MQ=255) cTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGt > 1:3091976/1‑71 (MQ=255) tGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGc > 1:5623445/1‑62 (MQ=255) ggggCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGc > 1:5555730/1‑50 (MQ=255) gggCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCa > 1:2219594/1‑70 (MQ=255) gggCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTct < 1:3094541/70‑2 (MQ=255) gggCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTct > 1:1850287/1‑69 (MQ=255) gCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTg < 1:1357902/61‑1 (MQ=255) gAGCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGt < 1:4493414/71‑1 (MQ=255) gCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGc < 1:383673/71‑1 (MQ=255) gCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCg < 1:463908/53‑1 (MQ=38) gCAAACCGACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGtac < 1:120337/71‑3 (MQ=255) aaCTGACGTACTGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCg > 1:2519185/1‑70 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCg > 1:3401781/1‑70 (MQ=255) accgACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCg > 1:468365/4‑69 (MQ=255) accgACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCa < 1:5522420/68‑1 (MQ=255) aCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCa < 1:2961473/71‑1 (MQ=255) ccgACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCt > 1:2336842/3‑51 (MQ=38) ccgACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCt > 1:2343359/3‑51 (MQ=38) ccgACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCt > 1:565916/3‑51 (MQ=38) cTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCt > 1:520417/1‑51 (MQ=255) tGACGTACCGGAATCTGCTGACTGGTACAACTCCa < 1:5091767/35‑1 (MQ=255) tGACGTACCGGAATCTGCTGACTGGTACAACTCCa < 1:5598279/35‑1 (MQ=255) tGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTgggg < 1:3502929/54‑1 (MQ=255) tGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACg > 1:3295964/1‑62 (MQ=255) tGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACg > 1:170113/1‑62 (MQ=255) cgACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGa > 1:1384451/2‑71 (MQ=255) gACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGa > 1:5269461/1‑70 (MQ=255) gACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGa > 1:781607/1‑70 (MQ=255) gACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGa > 1:1956245/1‑70 (MQ=255) gACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAc < 1:4100523/71‑1 (MQ=255) aCGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGAcg > 1:2852621/1‑71 (MQ=255) aCCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCg < 1:3213199/61‑1 (MQ=255) ccGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTAc > 1:2828340/1‑71 (MQ=255) cGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGAcgcg < 1:3832576/67‑1 (MQ=255) cGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCg < 1:1970589/59‑1 (MQ=38) ggAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCg < 1:4413453/58‑1 (MQ=38) gAATCTGCTGACTGGTACAACTCCAGCTACATCATCg > 1:3247520/1‑37 (MQ=255) gAATCTGCTGACTGGTACAACTCCAGCTACATCATCGc > 1:3921026/1‑38 (MQ=255) gAATCTGCTGACTGGTACAACTCCAGCTACATCATCGc > 1:1041930/1‑38 (MQ=255) gAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAcccc > 1:676253/1‑71 (MQ=255) gAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAcccc > 1:2135068/1‑71 (MQ=255) gAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAcc > 1:3576965/1‑69 (MQ=255) gAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCtaa > 1:938377/1‑50 (MQ=38) gAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCtaa > 1:2442026/1‑50 (MQ=38) gAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCtaa > 1:2908903/1‑50 (MQ=38) gAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTAcgcc < 1:935558/71‑1 (MQ=255) gAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTAcgcc < 1:878330/71‑1 (MQ=255) aaTCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGt < 1:5454331/65‑1 (MQ=255) aaTCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGt < 1:2089654/65‑1 (MQ=255) aTCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCgg < 1:3040704/71‑1 (MQ=255) tCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCaa < 1:2711814/46‑1 (MQ=255) tCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCaa < 1:251050/46‑1 (MQ=255) tCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCaa < 1:519489/46‑1 (MQ=255) tCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAc > 1:166102/1‑65 (MQ=255) tCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAc > 1:127842/1‑65 (MQ=255) tCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAc > 1:240491/1‑65 (MQ=255) ctgctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGAt > 1:2694472/1‑71 (MQ=255) tgctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATg > 1:4384016/1‑71 (MQ=255) tgccgACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGacg > 1:3068280/1‑69 (MQ=255) gctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGAt > 1:1039279/1‑69 (MQ=255) tgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCaa < 1:3941471/41‑1 (MQ=255) tgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCaa < 1:2425721/41‑1 (MQ=255) tgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCaa < 1:5640793/41‑1 (MQ=255) aCTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAAc > 1:1631243/1‑41 (MQ=255) tGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCAct > 1:3605696/1‑70 (MQ=255) ggTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCg > 1:1345933/1‑44 (MQ=255) ggTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCAct < 1:350138/69‑1 (MQ=255) gTATAACTCCAGTTACATCATCGCCTGGGGGTCTAACGta < 1:4712597/40‑2 (MQ=37) gTATAACTCCAGTTACATCATCGCCTGGGGGTCTAACGta < 1:4514718/40‑2 (MQ=37) gTATAACTCCAGTTACATCATCGCCTGGGGGTCTAACGta < 1:5528778/40‑2 (MQ=37) gTATAACTCCAGTTACATCATCGCCTGGGGGTCTAACGta < 1:429448/40‑2 (MQ=37) gTATAACTCCAGTTACATCATCGCCTGGGGGTCTAACGta < 1:422803/40‑2 (MQ=37) gTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCActtct > 1:3538926/1‑71 (MQ=255) gTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCActtc < 1:3426758/70‑1 (MQ=255) gTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCa < 1:2813539/45‑1 (MQ=255) gTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActt < 1:1521/69‑1 (MQ=255) tACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAcccc < 1:853902/56‑1 (MQ=255) tACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAcccc < 1:5281022/56‑1 (MQ=255) tACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:2389662/71‑1 (MQ=255) aCAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtctt > 1:5534822/1‑70 (MQ=255) | CGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTT > minE/832208‑832345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |