| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,334 | T→C | 37.4% | D264D (GAT→GAC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,334 | 0 | T | C | 37.4% | 76.4 / 96.7 | 99 | D264D (GAT→GAC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (29/33); new base C (19/18); total (48/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
CTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAA > minE/832264‑832404 | ctgctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGAt > 1:2694472/1‑71 (MQ=255) tgctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATg > 1:4384016/1‑71 (MQ=255) gctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGAt > 1:1039279/1‑69 (MQ=255) gctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCACGGAt > 1:2394206/1‑69 (MQ=255) tGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCAct > 1:3605696/1‑70 (MQ=255) ggTACAACTCCAGCTACATCATCGCCTGGGGGGCAAACGTGCCGCAGACGCGTACCCCGGATGCTCAct < 1:350138/69‑1 (MQ=255) gTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCActtct > 1:3538926/1‑71 (MQ=255) gTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCActtc < 1:3426758/70‑1 (MQ=255) gTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActt < 1:1521/69‑1 (MQ=255) tACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:2389662/71‑1 (MQ=255) aCAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtctt > 1:5534822/1‑70 (MQ=255) taaCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCttt < 1:4595201/69‑1 (MQ=255) taaCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCttt < 1:1688819/69‑1 (MQ=255) taaCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTa > 1:5046981/2‑71 (MQ=255) aaCTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCt < 1:2662608/60‑1 (MQ=255) aaCTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCt < 1:4730690/60‑1 (MQ=255) aCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCTCAGACACGTACGCCGGACGCCCACTTCTTTAcc > 1:3817375/1‑70 (MQ=255) tCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCActt < 1:1938902/62‑1 (MQ=255) tCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTAccg < 1:2584830/70‑3 (MQ=255) ccAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGAt > 1:1726886/1‑53 (MQ=255) cAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGa < 1:650272/69‑1 (MQ=255) cAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGa < 1:4799217/69‑1 (MQ=255) cctcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGt > 1:3551809/3‑70 (MQ=255) catcatCGCCTGGGGTTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtc > 1:4994262/1‑55 (MQ=255) catcatCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACgc < 1:4611402/70‑2 (MQ=255) catcatCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGt > 1:2484509/1‑70 (MQ=255) atcatcGCCTGGGGGTCTATCGTACCGCAGACACGTACGCCGGACGCCCActt < 1:134433/53‑1 (MQ=255) atcatcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTa > 1:656725/1‑71 (MQ=255) tcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTa > 1:2728192/1‑57 (MQ=255) catcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTAc > 1:5535609/1‑70 (MQ=255) catcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTAc > 1:4222716/1‑70 (MQ=255) atcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACa > 1:4172383/1‑70 (MQ=255) atcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACaa < 1:2475442/71‑1 (MQ=255) atcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACa > 1:1620070/1‑70 (MQ=255) atcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACa > 1:3839864/1‑70 (MQ=255) atcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACa > 1:2794444/1‑70 (MQ=255) tcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTAccg < 1:812926/57‑3 (MQ=255) tcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACAGAAGTACGCTACaaa > 1:2735956/1‑71 (MQ=255) tcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtc < 1:2392652/50‑1 (MQ=255) tcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACaaa < 1:3325938/71‑1 (MQ=255) cGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAg > 1:1353362/1‑71 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAg < 1:5040542/70‑1 (MQ=255) ggggTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCActa < 1:2941623/71‑3 (MQ=255) ggggTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCActa < 1:3623406/71‑3 (MQ=255) gggTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTaa < 1:5529506/71‑1 (MQ=255) gggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCAc > 1:4414876/1‑39 (MQ=255) gggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaa < 1:243501/71‑1 (MQ=255) gggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaa < 1:3847510/71‑1 (MQ=255) gggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaa < 1:1378252/71‑1 (MQ=255) ggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaaa < 1:2029313/71‑1 (MQ=255) tctaaCGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAAc < 1:5613126/68‑1 (MQ=255) cAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg > 1:113967/1‑61 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:5332486/39‑1 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:3573448/39‑1 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGaa > 1:1881890/1‑46 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg < 1:3776140/60‑1 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTg > 1:3052503/1‑71 (MQ=255) aaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaaa < 1:1482629/66‑1 (MQ=255) aCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGtc < 1:3143133/71‑2 (MQ=255) aCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGt < 1:164303/70‑1 (MQ=255) aCGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAg > 1:464371/1‑45 (MQ=255) aCGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAg > 1:2466735/1‑45 (MQ=255) aCGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAAc > 1:1477693/1‑67 (MQ=255) cGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:3762885/36‑1 (MQ=255) cGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAAc > 1:270907/1‑66 (MQ=255) cGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTg > 1:824326/1‑71 (MQ=255) gTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTg > 1:1725713/1‑70 (MQ=255) gTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTg > 1:2942999/1‑70 (MQ=255) gTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGc > 1:654801/1‑71 (MQ=255) tGCCGCAGACGCGTACCCCGGATGCTCACTTCtttt > 1:3518208/1‑35 (MQ=255) tGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTa > 1:4410367/1‑36 (MQ=255) tGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGcc > 1:350784/1‑71 (MQ=255) gCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCg > 1:2668483/1‑71 (MQ=255) ccGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:5044771/71‑1 (MQ=255) ccGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:2411531/71‑1 (MQ=255) aGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACaaa > 1:5423738/1‑47 (MQ=255) cgcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCa < 1:568557/71‑1 (MQ=255) cacgTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTaa > 1:2967376/3‑52 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCacac > 1:4665990/1‑68 (MQ=255) gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTg > 1:482381/1‑54 (MQ=255) gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:4666541/61‑1 (MQ=255) tACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACa < 1:2496207/38‑1 (MQ=255) aCCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACa < 1:4415845/37‑1 (MQ=255) cgccGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAACCATCGCCATTACCCCTGACTa > 1:1199696/3‑70 (MQ=255) ccccGGATGCTCACTTCTTTACTGAAGTGCGTTACa > 1:2666763/1‑36 (MQ=255) ccccGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAAc < 1:1543223/49‑1 (MQ=255) ccccGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAAc < 1:3603495/49‑1 (MQ=255) ccGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAAcca > 1:5219047/1‑47 (MQ=255) cGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAACCATCGCCATTACCCCTGACTActct > 1:2756726/1‑71 (MQ=255) cGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAACCATCGCCATTACCCCTGACTActct > 1:3479817/1‑71 (MQ=255) cGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAACCATCGCCATTACCCCTGACTActc > 1:259288/1‑68 (MQ=255) ggATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:4998232/54‑1 (MQ=255) ggATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:284573/54‑1 (MQ=255) ggATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGc > 1:1864301/1‑69 (MQ=255) ggACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAACCATCGCCATTACCCCTGACTACTCTg < 1:2691524/71‑1 (MQ=255) ggACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAACCATCGCCATTACCCCTGACTACTCTg < 1:5512562/71‑1 (MQ=255) gATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTg > 1:689125/1‑70 (MQ=255) aTGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaa < 1:4310725/71‑1 (MQ=255) tGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaaa < 1:2307398/71‑1 (MQ=255) tGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaa > 1:3174580/1‑70 (MQ=255) | CTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAA > minE/832264‑832404 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |