| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,376 | T→C | 6.2% | T278T (ACT→ACC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,376 | 0 | T | C | 6.2% | 278.0 / 5.7 | 96 | T278T (ACT→ACC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (49/41); new base C (4/2); total (53/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
AAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACA > minE/832307‑832441 | aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTg > 1:3052503/1‑71 (MQ=255) aCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGtc < 1:3143133/71‑2 (MQ=255) aCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGt < 1:164303/70‑1 (MQ=255) cGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTg > 1:824326/1‑71 (MQ=255) gTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTg > 1:2942999/1‑70 (MQ=255) gTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTg > 1:1725713/1‑70 (MQ=255) gTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGc > 1:654801/1‑71 (MQ=255) gTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGc > 1:2328699/1‑71 (MQ=255) tGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGcc > 1:350784/1‑71 (MQ=255) gCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCg > 1:2668483/1‑71 (MQ=255) ccGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:5044771/71‑1 (MQ=255) ccGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:2411531/71‑1 (MQ=255) cgcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCa < 1:568557/71‑1 (MQ=255) gcgTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCacac > 1:4665990/1‑68 (MQ=255) gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTg > 1:482381/1‑54 (MQ=255) gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:4666541/61‑1 (MQ=255) gTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGAc < 1:962101/71‑1 (MQ=255) cgccGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAACCATCGCCATTACCCCTGACTa > 1:1199696/3‑70 (MQ=255) cGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAACCATCGCCATTACCCCTGACTActct > 1:3479817/1‑71 (MQ=255) cGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAACCATCGCCATTACCCCTGACTActct > 1:2756726/1‑71 (MQ=255) cGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAACCATCGCCATTACCCCTGACTActc > 1:259288/1‑68 (MQ=255) ggATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:4998232/54‑1 (MQ=255) ggATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGt < 1:284573/54‑1 (MQ=255) ggATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGc > 1:1864301/1‑69 (MQ=255) ggACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAACCATCGCCATTACCCCTGACTACTCTg < 1:2691524/71‑1 (MQ=255) ggACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAACCATCGCCATTACCCCTGACTACTCTg < 1:5512562/71‑1 (MQ=255) gATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTg > 1:689125/1‑70 (MQ=255) aTGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaa < 1:4310725/71‑1 (MQ=255) tGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaaa < 1:2307398/71‑1 (MQ=255) tGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaa > 1:3174580/1‑70 (MQ=255) cTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTca > 1:5139528/1‑51 (MQ=255) cTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTca > 1:5423924/1‑51 (MQ=255) cTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTca > 1:3486655/1‑51 (MQ=255) tCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTg > 1:4794281/1‑41 (MQ=255) tCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTg > 1:2005069/1‑41 (MQ=255) tCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTg > 1:3764434/1‑41 (MQ=255) tCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCg > 1:3303168/1‑71 (MQ=255) cACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTa < 1:3966909/59‑1 (MQ=255) cACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTa < 1:5307474/59‑1 (MQ=255) aCTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGa > 1:5292437/1‑55 (MQ=255) aCTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGc > 1:2624974/1‑70 (MQ=255) aCTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGc > 1:2640918/1‑70 (MQ=255) ttcttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGcc < 1:2940867/42‑1 (MQ=255) tcttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaaa > 1:2717180/1‑71 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaaa < 1:2724908/70‑1 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAAc > 1:3395995/1‑71 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAAc > 1:270543/1‑71 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAAc > 1:1454583/1‑71 (MQ=255) cttTACTGAAGTGCGTTACAAAGGGACAAAAACTGTTGCCGTCACACCAGACTACGCTGAAATc > 1:3524381/1‑64 (MQ=255) tACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtg < 1:2003354/70‑1 (MQ=255) aCTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaa < 1:4275270/65‑1 (MQ=255) aCTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgtg > 1:2901675/1‑71 (MQ=255) aCTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgtg > 1:5422094/1‑71 (MQ=255) aCTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgt > 1:88103/1‑70 (MQ=255) cTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGc < 1:5286533/71‑1 (MQ=255) gAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGa > 1:5670754/1‑71 (MQ=255) aaGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAAc > 1:5057104/1‑63 (MQ=255) aaGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCg < 1:1822553/69‑1 (MQ=255) aaGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCg < 1:1851614/69‑1 (MQ=255) aGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATc > 1:1294859/1‑71 (MQ=255) aGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATc < 1:1794494/71‑1 (MQ=255) tGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGa < 1:517313/67‑1 (MQ=255) tGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCtg > 1:2701369/1‑71 (MQ=255) cGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaaa > 1:988673/1‑49 (MQ=255) ttACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGtaa < 1:2958244/47‑1 (MQ=255) ttACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaaa < 1:95249/47‑1 (MQ=255) ttACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGaaa < 1:2893078/47‑1 (MQ=255) ttACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgt < 1:617241/59‑1 (MQ=255) ttACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgt < 1:1713914/59‑1 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACTCTGAAATCGcc > 1:4828489/1‑50 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACTCTGAAATCGcc > 1:4834499/1‑50 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATc > 1:1235976/1‑47 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGcc > 1:2869033/1‑50 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaaa > 1:1711066/1‑53 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa < 1:5438222/51‑1 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa < 1:638277/51‑1 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa > 1:5662090/1‑51 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa < 1:3993389/51‑1 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCa < 1:3783935/51‑1 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggct < 1:2914552/70‑1 (MQ=255) aaaGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg > 1:2249693/1‑70 (MQ=255) aaaGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg > 1:4894099/1‑70 (MQ=255) aaGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg < 1:4086787/69‑1 (MQ=255) aGGGACCGAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCAc < 1:572442/71‑1 (MQ=255) ggACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCAcc > 1:4125620/1‑70 (MQ=255) ggACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCAcc > 1:1196310/1‑70 (MQ=255) gACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGa < 1:403498/71‑1 (MQ=255) gACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGa < 1:2448142/71‑1 (MQ=255) aCCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaa < 1:2999605/71‑1 (MQ=255) aCCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaa < 1:4646452/71‑1 (MQ=255) ccAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCaaa < 1:63704/44‑1 (MQ=255) ccAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa < 1:4162029/71‑1 (MQ=255) aaaaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa > 1:1259197/1‑69 (MQ=255) aaaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctggc < 1:2482576/61‑1 (MQ=255) aaaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCCCCGAAACa > 1:5118343/1‑70 (MQ=255) aaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAAc > 1:2172988/1‑41 (MQ=255) | AAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACA > minE/832307‑832441 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |