| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,413 | C→T | 11.8% | L291L (CTG→TTG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,413 | 0 | C | T | 11.8% | 296.7 / 22.0 | 111 | L291L (CTG→TTG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (43/54); new base T (8/5); total (51/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
CTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGT > minE/832343‑832480 | cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAAc > 1:270543/1‑71 (MQ=255)cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAAc > 1:3395995/1‑71 (MQ=255)cttTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAAc > 1:1454583/1‑71 (MQ=255) tACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtg < 1:2003354/70‑1 (MQ=255) aCTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgtg > 1:5422094/1‑71 (MQ=255) aCTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgt > 1:88103/1‑70 (MQ=255) cTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGc < 1:5286533/71‑1 (MQ=255) gAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGa > 1:5670754/1‑71 (MQ=255) aaGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCg < 1:1851614/69‑1 (MQ=255) aaGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCg < 1:1822553/69‑1 (MQ=255) aGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATc > 1:1294859/1‑71 (MQ=255) aGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATc < 1:1794494/71‑1 (MQ=255) tGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGa < 1:517313/67‑1 (MQ=255) ttACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACtgt < 1:617241/59‑1 (MQ=255) aCAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggct < 1:2914552/70‑1 (MQ=255) aaaGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg > 1:4894099/1‑70 (MQ=255) aaaGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg > 1:2249693/1‑70 (MQ=255) aaGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg < 1:4086787/69‑1 (MQ=255) aGGGACCGAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCAc < 1:572442/71‑1 (MQ=255) ggACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCAcc > 1:1196310/1‑70 (MQ=255) ggACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCAcc > 1:4125620/1‑70 (MQ=255) gACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGa < 1:403498/71‑1 (MQ=255) gACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGa < 1:2448142/71‑1 (MQ=255) aCCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaa < 1:4646452/71‑1 (MQ=255) aCCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaa < 1:2999605/71‑1 (MQ=255) ccAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa < 1:4162029/71‑1 (MQ=255) aaaaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa > 1:1259197/1‑69 (MQ=255) aaaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCCCCGAAACa > 1:5118343/1‑70 (MQ=255) aaCTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAAc > 1:2172988/1‑41 (MQ=255) gTTGCCGTCACACCAGACTACGCTGAAAACGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAc > 1:4448240/1‑71 (MQ=255) ttGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAgg < 1:3690683/66‑1 (MQ=255) ttGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:1910169/71‑1 (MQ=255) ccGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGAt < 1:3196267/71‑1 (MQ=255) tCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACa < 1:3756818/58‑1 (MQ=255) tCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACa < 1:894360/58‑1 (MQ=255) tCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGa < 1:3866823/67‑1 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCtg > 1:2355399/1‑40 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCtg > 1:2958692/1‑40 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGc > 1:2585835/1‑61 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCgg > 1:4275793/1‑71 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCgg > 1:2149322/1‑71 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCgg > 1:2155976/1‑71 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGAt > 1:4251374/1‑36 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGAt > 1:3032130/1‑36 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa < 1:2837562/54‑1 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGaaa < 1:2830678/54‑1 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAgg > 1:592428/1‑58 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGc < 1:5383525/71‑1 (MQ=255) acCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACa < 1:2392515/54‑1 (MQ=255) acCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCaa < 1:3964059/71‑1 (MQ=255) ccAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg < 1:4590889/43‑1 (MQ=255) ccAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAAc < 1:5030950/52‑1 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggc > 1:169297/1‑39 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGtggc > 1:5126283/1‑39 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGTACCGAAAc > 1:532683/1‑51 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAAc > 1:3821236/1‑51 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAAc > 1:4357905/1‑51 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATg > 1:4531944/1‑71 (MQ=255) aGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg > 1:1044184/1‑71 (MQ=255) gACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgc < 1:2195511/71‑1 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:4579405/56‑1 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:2506091/56‑1 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:2641041/56‑1 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg > 1:3295111/1‑71 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg > 1:4369683/1‑71 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg > 1:2937726/1‑71 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgc > 1:5640309/1‑70 (MQ=255) tACTCTGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCAct < 1:2780895/54‑2 (MQ=19) tACGCTGAAATCGCCAAACTGTGCGATCTGtggctgg < 1:2699977/37‑1 (MQ=255) tACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGa < 1:712533/43‑1 (MQ=255) tACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGa < 1:667677/43‑1 (MQ=255) tACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt > 1:1171134/1‑71 (MQ=255) cGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTgg > 1:4875849/1‑71 (MQ=255) tcTGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGAAAc < 1:1324339/42‑1 (MQ=16) gCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGc > 1:4841478/1‑71 (MQ=255) cTGAATTCGCCAAAGTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCg < 1:2510370/71‑1 (MQ=255) cTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGc < 1:771621/58‑1 (MQ=255) cTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGc < 1:3317750/58‑1 (MQ=255) tGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGAAAc > 1:1197339/1‑41 (MQ=16) tGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGAAAc > 1:5243065/1‑41 (MQ=16) tGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGAAAc > 1:5341849/1‑41 (MQ=16) tGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCAct > 1:2389869/1‑48 (MQ=19) tGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg < 1:2759535/62‑1 (MQ=255) tGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt < 1:471326/66‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:4716820/47‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:1202196/47‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGa < 1:30606/69‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:2656352/70‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATg < 1:4550437/71‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATg > 1:4135535/1‑71 (MQ=255) aaTCACCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCg < 1:216450/47‑1 (MQ=255) aTCGCCAAACTGTGCGATCTGTGGCTGGCCCCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggt > 1:1041489/1‑70 (MQ=255) aTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggg > 1:1459041/1‑71 (MQ=255) gCCAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCa > 1:5162204/1‑71 (MQ=255) ccAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCat < 1:4956967/71‑2 (MQ=255) aaaTTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCATGt < 1:3292348/71‑1 (MQ=255) aaaTTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCATGt < 1:4691403/71‑1 (MQ=255) aaaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt < 1:647448/71‑1 (MQ=255) cTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg > 1:726344/1‑49 (MQ=255) cTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg > 1:234138/1‑49 (MQ=255) | CTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGT > minE/832343‑832480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |