| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,442 | G→A | 10.4% | Q300Q (CAG→CAA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,442 | 0 | G | A | 10.4% | 235.9 / 13.3 | 86 | Q300Q (CAG→CAA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (35/42); new base A (4/5); total (39/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01 | |||||||||||
GTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCC > minE/832377‑832510 | gTTGCCGTCACACCAGACTACGCTGAAAACGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAc > 1:4448240/1‑71 (MQ=255) ttGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAgg < 1:669984/66‑1 (MQ=255) ttGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAgg < 1:3690683/66‑1 (MQ=255) ttGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:1910169/71‑1 (MQ=255) ccGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGAt < 1:3196267/71‑1 (MQ=255) tCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGa < 1:3866823/67‑1 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGc > 1:2585835/1‑61 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCgg > 1:2149322/1‑71 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCgg > 1:2155976/1‑71 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCgg > 1:4275793/1‑71 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAgg > 1:592428/1‑58 (MQ=255) acacCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGc < 1:5383525/71‑1 (MQ=255) acCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCaa < 1:3964059/71‑1 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATg > 1:4531944/1‑71 (MQ=255) cAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATg > 1:5291713/1‑71 (MQ=255) aGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg > 1:1044184/1‑71 (MQ=255) aGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg < 1:3169226/71‑1 (MQ=255) aGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg < 1:4082993/71‑1 (MQ=255) gACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgc < 1:2195511/71‑1 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:4579405/56‑1 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:2506091/56‑1 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:2056672/56‑1 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:2641041/56‑1 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg > 1:3504627/1‑71 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg > 1:3295111/1‑71 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg > 1:2937726/1‑71 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg > 1:4369683/1‑71 (MQ=255) aCTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgc > 1:5640309/1‑70 (MQ=255) tACTCTGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCAct < 1:2780895/54‑2 (MQ=19) tACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt > 1:1171134/1‑71 (MQ=255) cGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTgg > 1:4875849/1‑71 (MQ=255) cGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTgg < 1:2331287/71‑1 (MQ=255) gCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGc > 1:4841478/1‑71 (MQ=255) cTGAATTCGCCAAAGTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCg < 1:2510370/71‑1 (MQ=255) cTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGc < 1:771621/58‑1 (MQ=255) cTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGc < 1:3317750/58‑1 (MQ=255) tGAAGTGGCCAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCAct > 1:2389869/1‑48 (MQ=19) tGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg < 1:2759535/62‑1 (MQ=255) tGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCt < 1:471326/66‑1 (MQ=255) tGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGa > 1:4293305/1‑71 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:1202196/47‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCAcc < 1:4716820/47‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgcg > 1:4746651/1‑62 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGa < 1:30606/69‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAt < 1:2656352/70‑1 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATg > 1:4135535/1‑71 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATg < 1:4550437/71‑1 (MQ=255) aaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCg < 1:1224752/47‑1 (MQ=255) aaTCACCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCg < 1:216450/47‑1 (MQ=255) aTCGCCAAACTGTGCGATCTGTGGCTGGCCCCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggt > 1:1041489/1‑70 (MQ=255) aTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggg > 1:1459041/1‑71 (MQ=255) gCCAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCa > 1:5162204/1‑71 (MQ=255) ccAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCat < 1:4956967/71‑2 (MQ=255) cAAATTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCatg > 1:972398/1‑69 (MQ=255) aaaTTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCATGt < 1:3292348/71‑1 (MQ=255) aaaTTGTGCGACCAGTGGCTGGCACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATGGGCCATGt < 1:4691403/71‑1 (MQ=255) aaaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt < 1:647448/71‑1 (MQ=255) attgtgCGACCAGTGGCTGGCACCGAAACAAGGCACTGAt < 1:2578175/38‑1 (MQ=16) cTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg > 1:234138/1‑49 (MQ=255) cTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATgg > 1:726344/1‑49 (MQ=255) cGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTg > 1:1951876/1‑49 (MQ=255) cGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTg > 1:1306486/1‑49 (MQ=255) cGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTg > 1:4667873/1‑49 (MQ=255) cGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTg > 1:711005/1‑49 (MQ=255) cGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGc < 1:950805/51‑1 (MQ=255) cGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGc < 1:3870600/51‑1 (MQ=255) tCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGt > 1:1595232/1‑70 (MQ=255) cTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgc > 1:2328187/1‑41 (MQ=255) cTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGgc > 1:4393556/1‑41 (MQ=255) cTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGa > 1:5161605/1‑49 (MQ=255) tggctggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTg < 1:4393558/67‑1 (MQ=255) tggctggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGa < 1:3415584/68‑1 (MQ=255) ggctggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTg < 1:5047364/66‑1 (MQ=255) gctggcACCGAAACAAGGCACTGATAGCGCCCTGGCGATGGCAATg > 1:3183114/1‑46 (MQ=255) cACCGAAACAGGGCACCGATGCGGCAATGGCGCTgg < 1:5106141/36‑1 (MQ=255) aCCGAAACAGGGCTCCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGa < 1:545220/60‑1 (MQ=255) ccGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt < 1:3193421/47‑1 (MQ=255) ccGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt < 1:689026/47‑1 (MQ=255) ccGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGt < 1:2098150/47‑1 (MQ=255) ccGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTc < 1:2653088/69‑1 (MQ=255) cGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGa > 1:991128/1‑70 (MQ=255) cGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGAc < 1:2245183/71‑1 (MQ=255) gAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAtg > 1:1505257/1‑49 (MQ=255) gAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGAc > 1:2577609/1‑70 (MQ=255) aaCAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACaa > 1:5637108/1‑70 (MQ=255) aCAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGa < 1:4273547/54‑1 (MQ=255) cAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGAc < 1:2510651/66‑1 (MQ=255) cAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAAccc < 1:3708554/71‑1 (MQ=255) aGGGCACCGATGCGTCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGAc < 1:3677352/65‑1 (MQ=255) | GTTGCCGTCACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCC > minE/832377‑832510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |