| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,683 | A→G | 7.8% | N381D (AAT→GAT) ‡ | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,683 | 0 | A | G | 7.8% | 178.5 / 4.8 | 64 | N381D (AAT→GAT) ‡ | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (30/29); new base G (2/3); total (32/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01 | |||||||||||
TGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTG > minE/832616‑832752 | tgctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCaa > 1:4906603/1‑69 (MQ=255)tgctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATg > 1:390499/1‑71 (MQ=255) ctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGc > 1:475166/1‑71 (MQ=255) ctgATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGc > 1:5028076/1‑71 (MQ=255) ggTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATgg > 1:1609186/1‑71 (MQ=255) gTTGATGCGCTGGGCCAGTAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAAt < 1:2994825/68‑1 (MQ=255) gTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATg > 1:4598818/1‑61 (MQ=255) gTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGaaa < 1:3994189/67‑1 (MQ=255) gTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGaaa < 1:4205509/67‑1 (MQ=255) gTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAAt < 1:3972065/68‑1 (MQ=255) gTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAAt < 1:2212994/68‑1 (MQ=255) gTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATgg < 1:4221993/70‑1 (MQ=255) gTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATg < 1:2192330/69‑1 (MQ=255) gATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCg > 1:3111252/1‑61 (MQ=255) aTGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTgcg > 1:1871332/1‑71 (MQ=255) gcTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCg > 1:2032484/1‑70 (MQ=255) ggCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACgg > 1:2288422/1‑71 (MQ=255) aGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCg < 1:1852836/62‑1 (MQ=255) aGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCt < 1:486419/71‑1 (MQ=255) aGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACAAATGGCGAAATGGTTGCGCCGAACGGTTc > 1:1381824/1‑70 (MQ=255) ggAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGt > 1:797062/1‑54 (MQ=255) ggAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTgc < 1:612997/57‑1 (MQ=255) gAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGt > 1:287084/1‑53 (MQ=255) aaaaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTgcgc > 1:781686/1‑57 (MQ=255) aaaaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTgcgc > 1:2697215/1‑57 (MQ=255) aaaaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTAtt > 1:497840/1‑71 (MQ=255) aaaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTAt > 1:5661294/1‑69 (MQ=255) aaaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTAt > 1:5679711/1‑69 (MQ=255) aaaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTg > 1:3755832/1‑71 (MQ=255) aaaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTg > 1:4676397/1‑71 (MQ=255) aaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTgg > 1:309927/1‑71 (MQ=255) aaCAATCCGCAGTGGAAAACCGTAGCAGTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGTTCGAtcg > 1:1829555/1‑68 (MQ=255) aaCAATCCGCAGTGGAAAACCGTAGCAGTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGTTCGAtc > 1:1287352/1‑68 (MQ=255) tCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGt < 1:690011/45‑1 (MQ=255) ggAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACgg < 1:2235080/54‑1 (MQ=255) ggAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACgg < 1:222466/54‑1 (MQ=255) ggAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACgg < 1:1353414/54‑1 (MQ=255) ggAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCt < 1:3559558/71‑1 (MQ=255) agtGGAAAACCGTAGCAGTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGTTCGATTGGTTTCCGCTgg < 1:5462661/69‑1 (MQ=255) agtGGAAAACCGTAGCAGTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGTTCGATTGGTTTCCGCTgg < 1:2090708/69‑1 (MQ=255) agtGGAAAACCGTAGCAGTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGTTCGATTGGTTTCCGCTgg < 1:4568169/69‑1 (MQ=255) aaTGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTgg < 1:1443698/71‑1 (MQ=255) aTGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCt < 1:2674713/55‑1 (MQ=255) tGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAAc < 1:1467036/48‑1 (MQ=255) tGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTAtt < 1:3854500/57‑1 (MQ=255) ggAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTc < 1:5639540/52‑1 (MQ=255) gAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTgg > 1:4543940/1‑57 (MQ=255) gAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGc > 1:3160069/1‑70 (MQ=255) aaaCTGTCGCCTTTAATACCAATGGCGAAATGGtt > 1:2026243/1‑35 (MQ=255) aaCTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTggg < 1:4571973/65‑1 (MQ=255) aCTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGc > 1:2670162/1‑60 (MQ=255) gCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCaa < 1:3167222/71‑1 (MQ=255) gCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCa > 1:2257604/1‑70 (MQ=255) gCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCa > 1:3800650/1‑70 (MQ=255) ccTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTc < 1:888479/40‑1 (MQ=255) tAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGc > 1:1573237/1‑49 (MQ=255) tAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGc > 1:4182093/1‑49 (MQ=255) tAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGc > 1:5345206/1‑49 (MQ=255) tAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATgg > 1:4799910/1‑70 (MQ=255) tACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCg < 1:1178768/53‑1 (MQ=255) tACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATc < 1:4637853/71‑1 (MQ=255) aCCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCg < 1:2057519/52‑1 (MQ=255) cAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCtt < 1:1573428/70‑1 (MQ=255) cAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTg < 1:1672421/71‑1 (MQ=255) cAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTg < 1:4891240/71‑1 (MQ=255) | TGCTGATCTGGTTGATGCGCTGGGCCAGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTG > minE/832616‑832752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |