| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,712 | T→G | 27.9% | S390S (TCT→TCG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,712 | 0 | T | G | 27.9% | 93.7 / 52.2 | 68 | S390S (TCT→TCG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (31/18); new base G (3/16); total (34/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.70e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
AGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTGGCG > minE/832642‑832776 | aGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCt < 1:486419/71‑1 (MQ=255) aaaaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTAtt > 1:497840/1‑71 (MQ=255) aaaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTAt > 1:5679711/1‑69 (MQ=255) aaaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTAt > 1:5661294/1‑69 (MQ=255) aaaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTg > 1:3755832/1‑71 (MQ=255) aaaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTg > 1:4676397/1‑71 (MQ=255) aaCAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTgg > 1:309927/1‑71 (MQ=255) aaCAATCCGCAGTGGAAAACCGTAGCAGTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGTTCGAtcg > 1:1829555/1‑68 (MQ=255) aaCAATCCGCAGTGGAAAACCGTAGCAGTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGTTCGAtc > 1:1287352/1‑68 (MQ=255) ggAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCt < 1:3559558/71‑1 (MQ=255) agtGGAAAACCGTAGCAGTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGTTCGATTGGTTTCCGCTgg < 1:4568169/69‑1 (MQ=255) agtGGAAAACCGTAGCAGTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGTTCGATTGGTTTCCGCTgg < 1:5462661/69‑1 (MQ=255) agtGGAAAACCGTAGCAGTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGTTCGATTGGTTTCCGCTgg < 1:2090708/69‑1 (MQ=255) aaTGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTgg < 1:1443698/71‑1 (MQ=255) aTGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCt < 1:2674713/55‑1 (MQ=255) tGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTAtt < 1:3854500/57‑1 (MQ=255) gAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTgg > 1:4543940/1‑57 (MQ=255) gAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGc > 1:3160069/1‑70 (MQ=255) aaCTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTggg < 1:4571973/65‑1 (MQ=255) aCTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGc > 1:2670162/1‑60 (MQ=255) gCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCaa < 1:3167222/71‑1 (MQ=255) gCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCa > 1:3800650/1‑70 (MQ=255) gCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCa > 1:2257604/1‑70 (MQ=255) tAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGc > 1:5345206/1‑49 (MQ=255) tAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGc > 1:4182093/1‑49 (MQ=255) tAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGc > 1:1573237/1‑49 (MQ=255) tAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATgg > 1:4799910/1‑70 (MQ=255) tACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCg < 1:1178768/53‑1 (MQ=255) tACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATc < 1:4637853/71‑1 (MQ=255) aCCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCg < 1:2057519/52‑1 (MQ=255) cAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCtt < 1:1573428/70‑1 (MQ=255) cAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTg < 1:4891240/71‑1 (MQ=255) cAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTg < 1:1672421/71‑1 (MQ=255) gtgAATTGGTAGTGCCGAACGGTTCGATTGGTTTCCGCTGGGGAGAAAAAGGCAAATGGAATCt < 1:3070023/62‑1 (MQ=255) gCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATg > 1:2187086/1‑58 (MQ=255) gCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATg > 1:148097/1‑58 (MQ=255) gCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATg > 1:4714306/1‑58 (MQ=255) gAATTGGTAGTGCCGAACGGTTCGATTGGTTTCCGCTGGGGAGaaaaa > 1:5324752/1‑47 (MQ=16) aaaTGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTggg > 1:1987587/1‑39 (MQ=255) aaaTGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTggg > 1:4203655/1‑39 (MQ=255) aTGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGaa > 1:76384/1‑44 (MQ=255) aTGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGa < 1:1441733/71‑1 (MQ=255) tGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGAc > 1:5576061/1‑71 (MQ=255) ggTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGaa > 1:2464821/1‑42 (MQ=255) tGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCtt > 1:2226379/1‑55 (MQ=255) tGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCtt > 1:1557746/1‑55 (MQ=255) gcgcCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGagca < 1:1120922/59‑1 (MQ=255) tgcCGAACGGTTCGATTGGTTTCCGCTGGGGAGAAAAAGGCAAATGGa < 1:2862507/47‑1 (MQ=21) cgcCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGaa < 1:215322/37‑1 (MQ=255) cgcCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCaaa < 1:173592/71‑1 (MQ=255) cgcCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCaa < 1:1460577/70‑1 (MQ=255) gcCGAACGGTTCGATTGGTTTCCGCTGGGGAGAAAAAGGCa < 1:3965806/41‑1 (MQ=16) ccGAACGGTTCTATTGGTTTCCGCTGGGGAGAAAAAGGCAAATGGAATCtg > 1:2078621/1‑50 (MQ=37) ccGAACGGTTCGATTGGTTTCCGCTGGGGAGAAAAAGGCa < 1:2794787/40‑1 (MQ=16) ccGAACGGTTCGATTGGTTTCCGCTGGGGAGAAAAAGGCAAATGGa < 1:1606618/46‑1 (MQ=21) ccGAACGGTTCGATTGGTTTCCGCTGGGGAGAAAAAGGCAAATGGa < 1:879464/46‑1 (MQ=21) ccGAACGGTTCGATTGGTTTCCGCTGGGGAGAAAAAGGCAAATGGa < 1:3684995/46‑1 (MQ=21) cGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACt > 1:1844029/1‑71 (MQ=255) aaCGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTgg > 1:4364605/1‑71 (MQ=255) aaCGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTgg > 1:4600882/1‑71 (MQ=255) aaCGGTTCGATTGGTTTCCGCTGGGGAGAAAAAGGCAAATg < 1:2084685/41‑1 (MQ=16) aaCGGTTCGATTGGTTTCCGCTGGGGAGAAAAAGGCAAATg < 1:5512708/41‑1 (MQ=16) aaCGGTTCGATTGGTTTCCGCTGGGGAGAAAAAGGCAAATGGAAt < 1:1231966/45‑1 (MQ=21) aaCGGTTCGATTGGTTTCCGCTGGGGAGAAAAAGGCAAATGGAAt < 1:4563994/45‑1 (MQ=21) aaCGGTTCGATTGGTTTCCGCTGGGGAGAAAAAGGCAAATGGAAt < 1:3798485/45‑1 (MQ=21) cGGTTCTATTGGCTTCCGCTGGGGCGAGAACGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTGGCg < 1:61479/71‑1 (MQ=255) cGGTTCTATTGGCTTACGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTGGCg < 1:20356/71‑1 (MQ=255) ttCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCa > 1:2032609/1‑59 (MQ=255) ttCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAAc > 1:5263683/1‑63 (MQ=255) ttCGATTGGTTTCCGCTGGGGAGAAAAAGGCAAATGGaa < 1:622770/39‑1 (MQ=16) | AGGAAAACAATCCGGAATGGAAAACTGTCGCCTTTAATACCAATGGCGAAATGGTTGCGCCGAACGGTTCTATTGGCTTCCGCTGGGGCGAGAAGGGCAAATGGAATCTTGAGCAGCGCGACGGCAAAACTGGCG > minE/832642‑832776 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |