| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 833,132 | T→C | 13.7% | A530A (GCT→GCC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 833,132 | 0 | T | C | 13.7% | 176.8 / 17.9 | 73 | A530A (GCT→GCC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (33/30); new base C (8/2); total (41/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
GGCCGAGCAGATTACCGGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGA > minE/833063‑833197 | ggCCGAGCAGATTACCGGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCt > 1:496668/1‑70 (MQ=255) gAGCAGATTACCGGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATaa < 1:998684/71‑1 (MQ=255) gAGCAGATTACCGGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATaa < 1:812631/71‑1 (MQ=255) aGCAGATTACCGGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATaaa < 1:392896/71‑1 (MQ=255) cAGATTACCGGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAAc > 1:4503973/1‑71 (MQ=255) ccGGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACgg > 1:2061527/1‑70 (MQ=255) ccGGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGt > 1:989685/1‑71 (MQ=255) ggCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCg > 1:1446035/1‑71 (MQ=255) tttCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCg < 1:5458287/71‑1 (MQ=255) tcGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgatga > 1:4160535/1‑71 (MQ=255) tcGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgatga > 1:2032117/1‑71 (MQ=255) cGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgatgat > 1:4495796/1‑71 (MQ=255) gCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCgatgat > 1:3294832/1‑70 (MQ=255) gCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGAtt < 1:1660017/71‑1 (MQ=255) gCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGAtt < 1:4631708/71‑1 (MQ=255) aGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgtc > 1:4424897/1‑71 (MQ=255) attattCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgtcg > 1:3876212/1‑70 (MQ=255) attattCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCgg > 1:5486270/1‑71 (MQ=255) tattCGCATCGCCCGTGAATTTGCCGATAACGCTGa > 1:129051/1‑36 (MQ=255) tattCGCATCGCCCGTGAATTTGCCGATAACGCTGa > 1:3794782/1‑36 (MQ=255) tattCGCATCGCCCGTGAATTTGCCGATAACGCTGa > 1:2120276/1‑36 (MQ=255) tattCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGt > 1:3776606/1‑49 (MQ=255) attCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGc > 1:2310739/1‑71 (MQ=255) ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGc < 1:4779345/70‑1 (MQ=255) ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCg > 1:448964/1‑71 (MQ=255) ttCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCg > 1:4875783/1‑71 (MQ=255) ttCGCATCGCACGTGAATTTGCCGATAACGCTGATaa < 1:388856/37‑1 (MQ=255) ccATCGCCCGTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCagg > 1:3470908/2‑70 (MQ=255) tCGCCCGTGAATTTGCCGATACCGCCCATAAAACgt < 1:5321883/36‑2 (MQ=21) tCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt > 1:5553360/1‑70 (MQ=255) gCCCGTGAATTTGCCGATAGCGCTGATAAAACGCACGGTCGTTCGATGATTAtcgtc > 1:4249936/1‑57 (MQ=255) gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgtc > 1:1539867/1‑57 (MQ=255) gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa < 1:4305386/70‑1 (MQ=255) cccGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAAc < 1:1926014/71‑1 (MQ=255) gTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCgatg > 1:4957753/1‑44 (MQ=16) gTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCAc < 1:3346124/71‑1 (MQ=255) tGAATTTGCCGATAACGCTGATAAAACGCACGGTCg < 1:3733505/36‑1 (MQ=255) tGAATTTGCCGATAACGCTGATAAAACGCACGGTCg < 1:776077/36‑1 (MQ=255) tGAATTTGCCGATAACGCTGATAAAACGCACGGTCg < 1:2804959/36‑1 (MQ=255) tGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCg < 1:4147449/40‑1 (MQ=255) tGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCg < 1:5602389/40‑1 (MQ=255) tGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCg < 1:4947925/40‑1 (MQ=255) tGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCg < 1:1478583/40‑1 (MQ=255) tGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCg < 1:5063282/40‑1 (MQ=255) tGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCg < 1:4130145/40‑1 (MQ=255) tGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCg < 1:4379060/40‑1 (MQ=255) tGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCg < 1:2529688/40‑1 (MQ=255) gAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTg < 1:2100598/63‑1 (MQ=255) gAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTg < 1:725909/63‑1 (MQ=255) gAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTg > 1:3616516/1‑71 (MQ=255) aaTTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTAtcctc > 1:1112654/1‑50 (MQ=255) aaTTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTAtcctc > 1:2958871/1‑50 (MQ=255) aaTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTg < 1:3599317/62‑1 (MQ=255) aaTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg > 1:3454848/1‑71 (MQ=255) aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTAtcgtc > 1:963441/1‑49 (MQ=255) aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGt < 1:5615322/71‑1 (MQ=255) ttGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCgg > 1:2504301/1‑49 (MQ=255) tggcGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTAt > 1:5308718/4‑70 (MQ=255) tGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTAtc > 1:1594714/1‑43 (MQ=16) tGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCaggtg > 1:964578/1‑54 (MQ=255) tGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCa > 1:3023558/1‑63 (MQ=255) tGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTAt > 1:2204781/1‑70 (MQ=255) aTAACGCTGATAAAACGCACGGTCGTTCGATGATTAt > 1:5328840/1‑37 (MQ=255) aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc > 1:2843909/1‑71 (MQ=255) aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc > 1:511904/1‑71 (MQ=255) aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc > 1:5594759/1‑71 (MQ=255) aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc > 1:3836548/1‑71 (MQ=255) aaCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc < 1:3811269/69‑1 (MQ=255) aaCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc < 1:4694658/69‑1 (MQ=255) aaCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc < 1:3817943/69‑1 (MQ=255) aaCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGa > 1:3457660/1‑71 (MQ=255) aCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTAGGTGGGGGGCTGAACCACTGGTATCACCTc < 1:5588011/68‑1 (MQ=255) cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCg > 1:3274504/1‑68 (MQ=255) gCTGATAAAACGCACGGTCGTTCGATGATTAtcgt < 1:2252131/35‑1 (MQ=255) | GGCCGAGCAGATTACCGGCGTTTCTCGCAGCCAGATTATTCGCATCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGA > minE/833063‑833197 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |