Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,198 T→C 14.7% D552D (GAT→GAC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,1980TC14.7% 221.4 / 27.4 96D552D (GAT→GACnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (40/41);  new base C (9/5);  total (50/46)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

GATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGG  >  minE/833133‑833268
                                                                 |                                                                      
caTAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGaa                                                                   >  1:5055733/2‑71 (MQ=255)
 aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGa                                                                    <  1:988636/69‑1 (MQ=255)
 aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGa                                                                    <  1:3246245/69‑1 (MQ=255)
 aTAAAACGCACGGTCGTTCGATGATTATCGTAGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAAc                                                                  >  1:652935/1‑71 (MQ=255)
   aaaaCGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTa                                                                >  1:5373823/1‑71 (MQ=255)
   aaaaCGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTa                                                                >  1:5524510/1‑71 (MQ=255)
   aaaaCGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTa                                                                >  1:2336230/1‑71 (MQ=255)
   aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACt                                                                 >  1:114290/1‑70 (MQ=255)
   aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTa                                                                >  1:3878286/1‑71 (MQ=255)
   aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTa                                                                >  1:4628471/1‑71 (MQ=255)
      aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg                                                             >  1:1316998/1‑71 (MQ=255)
       cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGt                                                            >  1:669525/1‑71 (MQ=255)
         cACGGTCGTTCGATGATTATCGTCGGTGCGCGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                                                          <  1:1004468/71‑1 (MQ=255)
          aCGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGt                                                         <  1:3815336/71‑1 (MQ=255)
             gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                        <  1:5667402/69‑1 (MQ=255)
             gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                        <  1:349528/69‑1 (MQ=255)
             gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                        <  1:611356/69‑1 (MQ=255)
             gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                        <  1:1454271/69‑1 (MQ=255)
             gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCt                                                       >  1:1401083/1‑70 (MQ=255)
             gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                                                      <  1:5611212/71‑1 (MQ=255)
             gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                                                      <  1:1420865/71‑1 (MQ=255)
              tCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGa                                                     >  1:5689090/1‑71 (MQ=255)
              tCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGa                                                     >  1:4611107/1‑71 (MQ=255)
                gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg                                                             >  1:2467066/1‑61 (MQ=255)
                gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                                                    >  1:1571150/1‑70 (MQ=255)
                gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATc                                                   <  1:2996689/71‑1 (MQ=255)
                gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATc                                                   <  1:2989894/71‑1 (MQ=255)
                 ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                                                  <  1:1583912/71‑1 (MQ=255)
                 ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                                                  <  1:5547149/71‑1 (MQ=255)
                   cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                                                 >  1:1259031/1‑70 (MQ=255)
                   cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                                                 >  1:5430147/1‑70 (MQ=255)
                   cGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaa                                                 >  1:2968102/1‑70 (MQ=255)
                    gatgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaa                                                 <  1:5499255/69‑1 (MQ=255)
                     atgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAatat                                              <  1:4954499/71‑1 (MQ=255)
                      tgatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                                                 <  1:5172200/67‑1 (MQ=255)
                        atTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGc                                            <  1:3981034/70‑1 (MQ=255)
                         ttATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCt                                           >  1:4704277/1‑70 (MQ=255)
                            tcgtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATc                                                   >  1:1731914/1‑59 (MQ=255)
                            tcgtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                                                  >  1:5692257/1‑60 (MQ=255)
                            tcgtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAtt                                       >  1:5324934/1‑71 (MQ=255)
                            tcgtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAt                                        <  1:4684714/70‑1 (MQ=255)
                              gtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                        <  1:3251542/52‑1 (MQ=255)
                                    gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                                                          >  1:4609535/1‑44 (MQ=255)
                                    gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAtttt                                     >  1:2960422/1‑65 (MQ=255)
                                    gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAtttt                                     >  1:4236686/1‑65 (MQ=255)
                                    gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCgg                               >  1:5051978/1‑71 (MQ=255)
                                    gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCg                                >  1:5672695/1‑70 (MQ=255)
                                    gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCg                                >  1:4316618/1‑70 (MQ=255)
                                       gggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTg                                  <  1:1363383/65‑1 (MQ=255)
                                       gggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTg                                  <  1:5569653/65‑1 (MQ=255)
                                        ggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgt                           <  1:1035629/71‑1 (MQ=255)
                                         gCTGAACCACTGGTATGACCTCGATATGAACTATCGTGGTc                                                        <  1:1450204/41‑1 (MQ=255)
                                         gCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgtg                          >  1:3725412/1‑71 (MQ=255)
                                           ttaaCCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTGCGGTTgtgt                         <  1:3337644/68‑1 (MQ=255)
                                            gAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCg                       >  1:3202465/1‑71 (MQ=255)
                                             aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACAt                                              <  1:5208398/47‑1 (MQ=255)
                                             aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACAt                                              <  1:3254791/47‑1 (MQ=255)
                                             aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACAt                                              <  1:4789883/47‑1 (MQ=255)
                                             aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                    <  1:4140865/57‑1 (MQ=255)
                                             aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                    <  1:4707401/57‑1 (MQ=255)
                                             aaCCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTGCGGTTGTGTCgg                      >  1:2572011/1‑71 (MQ=255)
                                              aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                                                 <  1:2665655/43‑1 (MQ=255)
                                              aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                     <  1:575325/71‑1 (MQ=255)
                                              aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                     <  1:1917723/71‑1 (MQ=255)
                                              aCCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTGCGGTTGTGTCggg                     >  1:3094736/1‑70 (MQ=255)
                                               ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGc                                            >  1:2178360/1‑47 (MQ=255)
                                               ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGc                                            >  1:886426/1‑47 (MQ=255)
                                               ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGc                                            >  1:1368912/1‑47 (MQ=255)
                                               ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTg                                  >  1:4676367/1‑57 (MQ=255)
                                               ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTg                                  >  1:2864839/1‑57 (MQ=255)
                                               ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                     >  1:3930466/1‑70 (MQ=255)
                                               ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTc                    <  1:1400640/71‑1 (MQ=255)
                                               ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGGGCTGTGTCGGt                     >  1:5052111/1‑69 (MQ=255)
                                               ccACTGGTATCACCTCGATATGAACTATCGGGGTCTGATCAACATGCTGATTTTCTg                                  >  1:5203024/1‑57 (MQ=255)
                                               ccACTGGTATCACCTCGAAATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTg                                  >  1:3727123/1‑57 (MQ=255)
                                               ccACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTGCGGTTGTGTCgggc                    <  1:1205448/71‑3 (MQ=255)
                                               ccACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTGCGGTTGTGTCgggc                    <  1:4169593/71‑3 (MQ=255)
                                                cACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa                   <  1:1693458/71‑1 (MQ=255)
                                                 aCTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag                  <  1:3824254/71‑1 (MQ=255)
                                                 aCTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTGCGGTTGTGTCGGGCa                   >  1:5614519/1‑70 (MQ=255)
                                                     gTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCagag                <  1:763299/69‑1 (MQ=255)
                                                        tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                    <  1:5452341/46‑1 (MQ=255)
                                                        tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                    <  1:4202694/46‑1 (MQ=255)
                                                        tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGc               <  1:3514023/67‑1 (MQ=255)
                                                        tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCCGCTGTGTCGGTCAGAGc               <  1:577276/67‑1 (MQ=255)
                                                        tCACCTCGATATGAACTATCGTGATCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGc               <  1:3016432/67‑1 (MQ=255)
                                                         cACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGc                              <  1:1675534/51‑1 (MQ=255)
                                                         cACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGc                              <  1:5107298/51‑1 (MQ=255)
                                                          aCCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAtttt                                     >  1:2173763/1‑43 (MQ=255)
                                                          aCCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTg                                  >  1:5588664/1‑46 (MQ=255)
                                                          aCCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCgg             >  1:1604126/1‑67 (MQ=255)
                                                              cGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt     >  1:832257/1‑71 (MQ=255)
                                                               gATATGAACTATCGTGGTCTGATCAACATGCTGAtttt                                     >  1:3695424/1‑38 (MQ=255)
                                                               gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                    >  1:5221555/1‑39 (MQ=255)
                                                               gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                    >  1:884748/1‑39 (MQ=255)
                                                               gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTg    >  1:1200263/1‑71 (MQ=255)
                                                                atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa                   <  1:267822/55‑1 (MQ=255)
                                                                atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCgg             >  1:3879120/1‑61 (MQ=255)
                                                                atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGAgg             >  1:3948894/1‑61 (MQ=255)
                                                                 tatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTggg  <  1:4653097/71‑1 (MQ=255)
                                                                 |                                                                      
GATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGG  >  minE/833133‑833268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: