Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,207 T→C 10.2% Y555Y (TAT→TAC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,2070TC10.2% 260.5 / 16.3 98Y555Y (TAT→TACnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (43/45);  new base C (5/5);  total (48/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 102 total aligned reads displayed.

ACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATG  >  minE/833139‑833277
                                                                    |                                                                      
aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg                                                                      >  1:1316998/1‑71 (MQ=255)
 cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGt                                                                     >  1:669525/1‑71 (MQ=255)
   cACGGTCGTTCGATGATTATCGTCGGTGCGCGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                                                                   <  1:1004468/71‑1 (MQ=255)
    aCGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGt                                                                  <  1:3815336/71‑1 (MQ=255)
       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                                 <  1:611356/69‑1 (MQ=255)
       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                                 <  1:349528/69‑1 (MQ=255)
       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                                 <  1:1454271/69‑1 (MQ=255)
       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                                 <  1:5667402/69‑1 (MQ=255)
       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCt                                                                >  1:1401083/1‑70 (MQ=255)
       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                                                               <  1:1420865/71‑1 (MQ=255)
       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                                                               <  1:5611212/71‑1 (MQ=255)
        tCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGa                                                              >  1:5689090/1‑71 (MQ=255)
        tCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGa                                                              >  1:4611107/1‑71 (MQ=255)
          gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg                                                                      >  1:2467066/1‑61 (MQ=255)
          gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                                                             >  1:1571150/1‑70 (MQ=255)
          gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATc                                                            <  1:2996689/71‑1 (MQ=255)
          gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATc                                                            <  1:2989894/71‑1 (MQ=255)
           ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                                                           <  1:1583912/71‑1 (MQ=255)
           ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                                                           <  1:5547149/71‑1 (MQ=255)
             cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                                                          >  1:1259031/1‑70 (MQ=255)
             cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                                                          >  1:5430147/1‑70 (MQ=255)
             cGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaa                                                          >  1:2968102/1‑70 (MQ=255)
              gatgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaa                                                          <  1:5499255/69‑1 (MQ=255)
               atgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAatat                                                       <  1:4954499/71‑1 (MQ=255)
                tgatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                                                          <  1:5172200/67‑1 (MQ=255)
                  atTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGc                                                     <  1:3981034/70‑1 (MQ=255)
                   ttATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCt                                                    >  1:4704277/1‑70 (MQ=255)
                      tcgtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATc                                                            >  1:1731914/1‑59 (MQ=255)
                      tcgtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                                                           >  1:5692257/1‑60 (MQ=255)
                      tcgtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAtt                                                >  1:5324934/1‑71 (MQ=255)
                      tcgtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAt                                                 <  1:4684714/70‑1 (MQ=255)
                        gtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                                                 <  1:3251542/52‑1 (MQ=255)
                              gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                                                                   >  1:4609535/1‑44 (MQ=255)
                              gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAtttt                                              >  1:4236686/1‑65 (MQ=255)
                              gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAtttt                                              >  1:2960422/1‑65 (MQ=255)
                              gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCgg                                        >  1:5051978/1‑71 (MQ=255)
                              gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCg                                         >  1:4316618/1‑70 (MQ=255)
                              gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCg                                         >  1:5672695/1‑70 (MQ=255)
                                 gggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTg                                           <  1:5569653/65‑1 (MQ=255)
                                 gggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTg                                           <  1:1363383/65‑1 (MQ=255)
                                  ggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgt                                    <  1:1035629/71‑1 (MQ=255)
                                   gCTGAACCACTGGTATGACCTCGATATGAACTATCGTGGTc                                                                 <  1:1450204/41‑1 (MQ=255)
                                   gCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgtg                                   >  1:3725412/1‑71 (MQ=255)
                                     ttaaCCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTGCGGTTgtgt                                  <  1:3337644/68‑1 (MQ=255)
                                      gAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCg                                >  1:3202465/1‑71 (MQ=255)
                                       aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACAt                                                       <  1:3254791/47‑1 (MQ=255)
                                       aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACAt                                                       <  1:5208398/47‑1 (MQ=255)
                                       aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACAt                                                       <  1:4789883/47‑1 (MQ=255)
                                       aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                             <  1:4707401/57‑1 (MQ=255)
                                       aaCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                             <  1:4140865/57‑1 (MQ=255)
                                       aaCCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTGCGGTTGTGTCgg                               >  1:2572011/1‑71 (MQ=255)
                                        aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                                                          <  1:2665655/43‑1 (MQ=255)
                                        aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                              <  1:575325/71‑1 (MQ=255)
                                        aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                              <  1:1917723/71‑1 (MQ=255)
                                        aCCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTGCGGTTGTGTCggg                              >  1:3094736/1‑70 (MQ=255)
                                         ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGc                                                     >  1:1368912/1‑47 (MQ=255)
                                         ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGc                                                     >  1:2178360/1‑47 (MQ=255)
                                         ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTg                                           >  1:4676367/1‑57 (MQ=255)
                                         ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTg                                           >  1:2864839/1‑57 (MQ=255)
                                         ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt                              >  1:3930466/1‑70 (MQ=255)
                                         ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTc                             <  1:1400640/71‑1 (MQ=255)
                                         ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGGGCTGTGTCGGt                              >  1:5052111/1‑69 (MQ=255)
                                         ccACTGGTATCACCTCGATATGAACTATCGGGGTCTGATCAACATGCTGATTTTCTg                                           >  1:5203024/1‑57 (MQ=255)
                                         ccACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTGCGGTTGTGTCgggc                             <  1:4169593/71‑3 (MQ=255)
                                         ccACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTGCGGTTGTGTCgggc                             <  1:1205448/71‑3 (MQ=255)
                                          cACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa                            <  1:1693458/71‑1 (MQ=255)
                                           aCTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag                           <  1:3824254/71‑1 (MQ=255)
                                           aCTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTGCGGTTGTGTCGGGCa                            >  1:5614519/1‑70 (MQ=255)
                                               gTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCagag                         <  1:763299/69‑1 (MQ=255)
                                                  tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                             <  1:5452341/46‑1 (MQ=255)
                                                  tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                             <  1:4202694/46‑1 (MQ=255)
                                                  tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGc                        <  1:3514023/67‑1 (MQ=255)
                                                  tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCCGCTGTGTCGGTCAGAGc                        <  1:577276/67‑1 (MQ=255)
                                                  tCACCTCGATATGAACTATCGTGATCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGc                        <  1:3016432/67‑1 (MQ=255)
                                                   cACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGc                                       <  1:5107298/51‑1 (MQ=255)
                                                   cACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGc                                       <  1:1675534/51‑1 (MQ=255)
                                                    aCCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAtttt                                              >  1:2173763/1‑43 (MQ=255)
                                                    aCCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTg                                           >  1:5588664/1‑46 (MQ=255)
                                                    aCCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCgg                      >  1:1604126/1‑67 (MQ=255)
                                                        cGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt              >  1:832257/1‑71 (MQ=255)
                                                         gATATGAACTATCGTGGTCTGATCAACATGCTGAtttt                                              >  1:3695424/1‑38 (MQ=255)
                                                         gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                             >  1:884748/1‑39 (MQ=255)
                                                         gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTc                                             >  1:5221555/1‑39 (MQ=255)
                                                         gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTg             >  1:1200263/1‑71 (MQ=255)
                                                          atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa                            <  1:267822/55‑1 (MQ=255)
                                                          atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCgg                      >  1:3879120/1‑61 (MQ=255)
                                                          atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGAgg                      >  1:3948894/1‑61 (MQ=255)
                                                           tatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTggg           <  1:4653097/71‑1 (MQ=255)
                                                             tGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag                           <  1:2083719/53‑1 (MQ=255)
                                                              gAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCggg                     >  1:47930/1‑58 (MQ=255)
                                                                aCTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCAc      >  1:3275974/1‑71 (MQ=255)
                                                                 cTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGc                        >  1:3281777/1‑52 (MQ=255)
                                                                  tATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag                           <  1:2566668/48‑1 (MQ=255)
                                                                  tATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag                           <  1:2477942/48‑1 (MQ=255)
                                                                  tATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag                           <  1:2191825/48‑1 (MQ=255)
                                                                   aTCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTAt   >  1:1231917/1‑71 (MQ=255)
                                                                    tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTa    <  1:2326530/69‑1 (MQ=255)
                                                                    tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg  >  1:407809/1‑71 (MQ=255)
                                                                    tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg  >  1:2480508/1‑71 (MQ=255)
                                                                    tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGAGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg  <  1:472933/71‑1 (MQ=255)
                                                                    |                                                                      
ACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATG  >  minE/833139‑833277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: