| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 833,249 | T→G | 25.9% | G569G (GGT→GGG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 833,249 | 0 | T | G | 25.9% | 102.0 / 62.6 | 85 | G569G (GGT→GGG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (34/29); new base G (16/6); total (50/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.06e-01 | |||||||||||
ACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGC > minE/833179‑833319 | aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt < 1:1917723/71‑1 (MQ=255)aCCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt < 1:575325/71‑1 (MQ=255) ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGt > 1:3930466/1‑70 (MQ=255) ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTc < 1:1400640/71‑1 (MQ=255) ccACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTG‑GGCTGTGTCGGt > 1:5052111/1‑69 (MQ=255) cACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa < 1:1693458/71‑1 (MQ=255) aCTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag < 1:3824254/71‑1 (MQ=255) aCTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCTGATCTTCTGCGGTTGTGTCGGGCa > 1:5614519/1‑70 (MQ=255) gTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCagag < 1:763299/69‑1 (MQ=255) tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGc < 1:3514023/67‑1 (MQ=255) tCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCCGCTGTGTCGGTCAGAGc < 1:577276/67‑1 (MQ=255) tCACCTCGATATGAACTATCGTGATCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGc < 1:3016432/67‑1 (MQ=255) aCCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCgg > 1:1604126/1‑67 (MQ=255) cGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt > 1:832257/1‑71 (MQ=255) gATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTg > 1:1200263/1‑71 (MQ=255) atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa < 1:267822/55‑1 (MQ=255) atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCgg > 1:3879120/1‑61 (MQ=255) atatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGAgg > 1:3948894/1‑61 (MQ=255) tatGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTggg < 1:4653097/71‑1 (MQ=255) tGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag < 1:2083719/53‑1 (MQ=255) gAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCggg > 1:47930/1‑58 (MQ=255) aCTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCAc > 1:3275974/1‑71 (MQ=255) cTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGc > 1:3281777/1‑52 (MQ=255) tATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag < 1:2477942/48‑1 (MQ=255) tATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag < 1:2191825/48‑1 (MQ=255) tATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag < 1:2566668/48‑1 (MQ=255) aTCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTAt > 1:1231917/1‑71 (MQ=255) tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTa < 1:2326530/69‑1 (MQ=255) tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg > 1:2480508/1‑71 (MQ=255) tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg > 1:407809/1‑71 (MQ=255) tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGAGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg < 1:472933/71‑1 (MQ=255) gTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGt < 1:4566378/71‑1 (MQ=255) gTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGt < 1:1916905/71‑1 (MQ=255) tCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGt > 1:806225/1‑70 (MQ=255) cTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa > 1:3725610/1‑38 (MQ=255) cTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa > 1:4474075/1‑38 (MQ=255) cTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCa > 1:1198842/1‑38 (MQ=255) tGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCag < 1:1527186/38‑1 (MQ=255) aTCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAg > 1:2821760/1‑69 (MQ=255) aTCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGa < 1:2944028/71‑1 (MQ=255) aTCAACATGCTGATTTTCTGCGGCTGTGTCGGTCagag < 1:2509602/38‑1 (MQ=255) tCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTa > 1:5152176/1‑58 (MQ=255) tCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTa > 1:3851377/1‑58 (MQ=255) tCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTa > 1:3857956/1‑58 (MQ=255) tCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTa > 1:49515/1‑58 (MQ=255) tCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTa > 1:2691954/1‑58 (MQ=255) cAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGgc > 1:5581015/1‑51 (MQ=255) aataTGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaa < 1:4184778/68‑1 (MQ=255) aataTGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaa < 1:3016967/68‑1 (MQ=255) aataTGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaa > 1:2981445/4‑70 (MQ=255) aaCATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaa < 1:3493599/70‑1 (MQ=255) aaCATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaa < 1:2167987/70‑1 (MQ=255) aTGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAg > 1:188112/1‑58 (MQ=255) aTGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAg > 1:1262426/1‑58 (MQ=255) aTGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAg > 1:4690972/1‑58 (MQ=255) aTGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaaa > 1:3406557/1‑69 (MQ=255) aTGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaaa > 1:4464413/1‑69 (MQ=255) aTGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaaa > 1:4304371/1‑69 (MQ=255) tGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCggggg > 1:1931444/1‑37 (MQ=255) tGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCggggg > 1:1402031/1‑37 (MQ=255) tGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACt < 1:5102118/51‑1 (MQ=255) tGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaaa > 1:4945892/1‑68 (MQ=255) tGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTg > 1:3034003/1‑71 (MQ=255) atcttCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaaa > 1:3438767/4‑63 (MQ=255) aTTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt < 1:5604210/37‑1 (MQ=255) aTTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt < 1:3063288/37‑1 (MQ=255) ttttCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt < 1:4352605/36‑1 (MQ=255) ttttCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCt < 1:2520814/36‑1 (MQ=255) tttCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTgg > 1:2816984/1‑37 (MQ=255) tttCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCg > 1:1400618/1‑70 (MQ=255) cttCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGccca > 1:949659/2‑69 (MQ=255) tCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCa < 1:5222416/70‑1 (MQ=255) tGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCa > 1:1644431/1‑38 (MQ=255) tGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAAc > 1:2936667/1‑58 (MQ=255) tGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAAc > 1:4963301/1‑58 (MQ=255) gCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaa > 1:3734738/1‑54 (MQ=255) ggCTGTTTCGGTCAGAGCGGGGGCGGCTGGGCGCTCTATGTa > 1:2750740/1‑42 (MQ=38) ggCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGt > 1:3400123/1‑45 (MQ=255) ggCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGt > 1:3760021/1‑45 (MQ=255) gtgtCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTgg > 1:5529711/1‑71 (MQ=255) tgtCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTgg < 1:1003885/70‑1 (MQ=255) tgtCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTggt < 1:4121250/71‑2 (MQ=255) tgtCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTgg < 1:1119725/70‑1 (MQ=255) tCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAAc > 1:1166919/1‑48 (MQ=255) tCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCaa < 1:2999885/59‑1 (MQ=255) tCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCaa < 1:1294849/59‑1 (MQ=255) cGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGc > 1:5440272/1‑71 (MQ=255) ggTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAAc > 1:3649610/1‑46 (MQ=255) ggTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAAc > 1:3089178/1‑46 (MQ=255) tCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGcccc < 1:3377181/71‑3 (MQ=255) | ACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGC > minE/833179‑833319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |