| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 833,270 | G→A | 45.7% | A576A (GCG→GCA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 833,270 | 0 | G | A | 45.7% | 20.1 / 81.4 | 70 | A576A (GCG→GCA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (23/15); new base A (18/14); total (41/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGG > minE/833203‑833339 | aCTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCAc > 1:3275974/1‑71 (MQ=255) aTCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTAt > 1:1231917/1‑71 (MQ=255) tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTa < 1:2326530/69‑1 (MQ=255) tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg > 1:2480508/1‑71 (MQ=255) tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg > 1:407809/1‑71 (MQ=255) tCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGAGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATg < 1:472933/71‑1 (MQ=255) gTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGt < 1:4566378/71‑1 (MQ=255) gTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGt < 1:1916905/71‑1 (MQ=255) tCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGt > 1:806225/1‑70 (MQ=255) aTCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAg > 1:2821760/1‑69 (MQ=255) aTCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGa < 1:2944028/71‑1 (MQ=255) tCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTa > 1:2691954/1‑58 (MQ=255) tCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTa > 1:49515/1‑58 (MQ=255) tCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTa > 1:3851377/1‑58 (MQ=255) tCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTa > 1:5152176/1‑58 (MQ=255) tCAATATGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTa > 1:3857956/1‑58 (MQ=255) aataTGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaa < 1:3016967/68‑1 (MQ=255) aataTGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaa < 1:4184778/68‑1 (MQ=255) aataTGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaa > 1:2981445/4‑70 (MQ=255) aaCATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaa < 1:3493599/70‑1 (MQ=255) aaCATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaa < 1:2167987/70‑1 (MQ=255) aTGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAg > 1:4690972/1‑58 (MQ=255) aTGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAg > 1:188112/1‑58 (MQ=255) aTGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAg > 1:1262426/1‑58 (MQ=255) aTGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaaa > 1:4464413/1‑69 (MQ=255) aTGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaaa > 1:4304371/1‑69 (MQ=255) aTGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaaa > 1:3406557/1‑69 (MQ=255) tGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACt < 1:5102118/51‑1 (MQ=255) tGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaaa > 1:4945892/1‑68 (MQ=255) tGCTGATCTTCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTg > 1:3034003/1‑71 (MQ=255) atcttCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGaaaaa > 1:3438767/4‑63 (MQ=255) tttCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCg > 1:1400618/1‑70 (MQ=255) cttCTGCGGTTGTGTCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGccca > 1:949659/2‑69 (MQ=255) tCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCa < 1:5222416/70‑1 (MQ=255) tGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCa > 1:1644431/1‑38 (MQ=255) tGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAAc > 1:4963301/1‑58 (MQ=255) tGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAAc > 1:2936667/1‑58 (MQ=255) gCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGaaa > 1:3734738/1‑54 (MQ=255) ggCTGTTTCGGTCAGAGCGGGGGCGGCTGGGCGCTCTATGTa > 1:2750740/1‑42 (MQ=38) ggCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGt > 1:3400123/1‑45 (MQ=255) ggCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGt > 1:3760021/1‑45 (MQ=255) gtgtCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTgg > 1:5529711/1‑71 (MQ=255) tgtCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTgg < 1:1003885/70‑1 (MQ=255) tgtCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTggt < 1:4121250/71‑2 (MQ=255) tgtCGGGCAAAGCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTgg < 1:1119725/70‑1 (MQ=255) tCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAAc > 1:1166919/1‑48 (MQ=255) tCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCaa < 1:2999885/59‑1 (MQ=255) tCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCaa < 1:1294849/59‑1 (MQ=255) cGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGc > 1:5440272/1‑71 (MQ=255) ggTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAAc > 1:3649610/1‑46 (MQ=255) ggTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAAc > 1:3089178/1‑46 (MQ=255) tCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGcccc < 1:3377181/71‑3 (MQ=255) aaagCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTg < 1:616480/69‑1 (MQ=255) agCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGc < 1:4208992/71‑1 (MQ=255) gCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGcc < 1:2457101/71‑2 (MQ=255) cGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGcct < 1:2909029/71‑3 (MQ=255) tggcggcTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTg < 1:244647/70‑1 (MQ=255) gcggcTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGcg > 1:4792371/1‑70 (MQ=255) gcggcTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGcg < 1:2734165/71‑2 (MQ=255) gcggcTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGcg < 1:590398/71‑2 (MQ=255) gcggcTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGcg < 1:1126363/71‑2 (MQ=255) gcggcTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGc > 1:96120/1‑70 (MQ=255) ggcTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCt > 1:5004806/1‑71 (MQ=255) gcTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCtt < 1:3890155/71‑1 (MQ=255) cTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGCGCtcg < 1:1484523/71‑3 (MQ=255) cTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGCGCtc > 1:385212/1‑69 (MQ=255) ggCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTgg > 1:907848/1‑46 (MQ=255) ggCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGAc < 1:1988872/70‑1 (MQ=255) ggCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGAc < 1:21541/70‑1 (MQ=255) ggCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACt > 1:2654840/1‑71 (MQ=255) gcACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTgg > 1:3041872/1‑70 (MQ=255) | ACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTGTCGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGG > minE/833203‑833339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |