Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,313 C→T 41.2% Q591* (CAG→TAG) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,3130CT41.2% 30.1 / 52.1 51Q591* (CAG→TAG) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (14/16);  new base T (8/13);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 5.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTC  >  minE/833246‑833378
                                                                   |                                                                 
cGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGc                                                                >  1:5440272/1‑71 (MQ=255)
   tCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGcccc                                                             <  1:3377181/71‑3 (MQ=255)
     aaagCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTg                                                           <  1:616480/69‑1 (MQ=255)
       agCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGc                                                         <  1:4208992/71‑1 (MQ=255)
        gCGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGcc                                                        <  1:2457101/71‑2 (MQ=255)
         cGGTGGCGGCTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGcct                                                       <  1:2909029/71‑3 (MQ=255)
            tggcggcTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTg                                                    <  1:244647/70‑1 (MQ=255)
              gcggcTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGcg                                                  <  1:2734165/71‑2 (MQ=255)
              gcggcTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGcg                                                  <  1:1126363/71‑2 (MQ=255)
              gcggcTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGcg                                                  <  1:590398/71‑2 (MQ=255)
              gcggcTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGcg                                                  >  1:4792371/1‑70 (MQ=255)
              gcggcTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGc                                                   >  1:96120/1‑70 (MQ=255)
                ggcTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCt                                                >  1:5004806/1‑71 (MQ=255)
                 gcTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCtt                                               <  1:3890155/71‑1 (MQ=255)
                  cTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGCGCtcg                                              <  1:1484523/71‑3 (MQ=255)
                  cTGGGCACACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGCGCtc                                               >  1:385212/1‑69 (MQ=255)
                     ggCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGAc                                            <  1:1988872/70‑1 (MQ=255)
                     ggCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGAc                                            <  1:21541/70‑1 (MQ=255)
                     ggCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACt                                           >  1:2654840/1‑71 (MQ=255)
                       cacACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTgg                                         <  1:1095538/69‑1 (MQ=255)
                       cacACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTgg                                         <  1:1788640/69‑1 (MQ=255)
                        gcACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTgg                                         >  1:3041872/1‑70 (MQ=255)
                        acACTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTgg                                         >  1:5470204/2‑70 (MQ=255)
                          aCTATGTCGGCCAGGAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTggaac                                      <  1:1860594/71‑4 (MQ=255)
                          aCTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCtt                                               >  1:1999443/1‑62 (MQ=255)
                          aCTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAg                                      <  1:2991167/71‑1 (MQ=255)
                            tATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCg                                    >  1:1920352/1‑71 (MQ=255)
                                tAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTcc                                 <  1:1339780/70‑1 (MQ=255)
                                        gAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGgc                              <  1:3114799/65‑1 (MQ=255)
                                        gAAAAACTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGCGCtc                                               >  1:3578442/1‑47 (MQ=16)
                                          aaaaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTcaca                      >  1:2860713/1‑71 (MQ=255)
                                           aaaCTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGCGCtc                                               <  1:922362/45‑2 (MQ=12)
                                            aaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg                    <  1:3706132/71‑1 (MQ=255)
                                            aaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg                    <  1:5646108/71‑1 (MQ=255)
                                            aaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg                    <  1:3900721/71‑1 (MQ=255)
                                            aaCTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATg                    <  1:1513747/71‑1 (MQ=255)
                                             aCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTc                                  >  1:5407000/1‑56 (MQ=255)
                                                  gTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAg               >  1:2884735/1‑70 (MQ=255)
                                                   tCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTca                        <  1:2669784/60‑1 (MQ=255)
                                                   tCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCa             >  1:327492/1‑71 (MQ=255)
                                                     cacAAACCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATGAAcaccac            >  1:657669/3‑70 (MQ=255)
                                                     cGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCAct           >  1:2537324/1‑71 (MQ=255)
                                                      acAAACCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCAcc           >  1:5181827/2‑69 (MQ=255)
                                                        aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg                    <  1:1340339/59‑1 (MQ=255)
                                                        aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCActtct        <  1:5119343/71‑1 (MQ=255)
                                                        aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCActtct        <  1:5112745/71‑1 (MQ=255)
                                                        aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCActtct        <  1:1205984/71‑1 (MQ=255)
                                                          aCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGaa                  >  1:1047678/1‑59 (MQ=255)
                                                          aCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTa      >  1:697915/1‑71 (MQ=255)
                                                              gCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTc  >  1:4419780/1‑71 (MQ=255)
                                                              gCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTc  >  1:17889/1‑71 (MQ=255)
                                                                   |                                                                 
CGGTCAGAGCGGGGGCGGCTGGGCGCACTATGTAGGTCAGGAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTC  >  minE/833246‑833378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: