| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 833,381 | T→C | 14.3% | Y613Y (TAT→TAC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 833,381 | 0 | T | C | 14.3% | 146.6 / 15.0 | 63 | Y613Y (TAT→TAC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (31/23); new base C (4/5); total (35/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
CGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACA > minE/833323‑833448 | cGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagc < 1:1808947/71‑1 (MQ=255) gTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagcc < 1:5274879/71‑1 (MQ=255) ctttGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagcc < 1:3783453/70‑1 (MQ=255) tttGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCa < 1:1780901/62‑1 (MQ=255) ttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagccag > 1:926560/1‑71 (MQ=255) ttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagccag > 1:3296858/1‑71 (MQ=255) tGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagcc < 1:1474575/68‑1 (MQ=255) tGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagcc < 1:2288136/68‑1 (MQ=255) ccTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGc < 1:1856097/71‑1 (MQ=255) ccTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGc > 1:4594606/1‑71 (MQ=255) ttGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGt > 1:4556130/1‑71 (MQ=255) tGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGt > 1:3218542/1‑70 (MQ=255) gACTGGCAGCGTCCGGCTCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagcca > 1:2850527/1‑62 (MQ=255) gACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCa > 1:436715/1‑53 (MQ=255) gACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagccaa > 1:3442579/1‑62 (MQ=255) gACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTCCAGCCAATGGCGCTa > 1:1517193/1‑71 (MQ=255) cTGGCAGCGTCCGGCGCGTCCCATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTAt > 1:706975/1‑70 (MQ=255) gCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaaa < 1:4814578/71‑1 (MQ=255) gCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATATCTATAACCa > 1:2405054/1‑48 (MQ=255) cAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAc > 1:416713/1‑71 (MQ=255) cAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAc > 1:674545/1‑71 (MQ=255) aGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAcc < 1:3192181/71‑1 (MQ=255) tCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaa < 1:1168531/64‑1 (MQ=255) tCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaa < 1:3531251/64‑1 (MQ=255) caccgcgCCAAATGAACAGCACCTCGTTTTTCTACAATCATTCCAGCCAATGGCGCTATGaaa < 1:4525448/60‑1 (MQ=255) cGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTa > 1:4357240/1‑58 (MQ=255) ggCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcgg > 1:114987/1‑71 (MQ=255) gcgcCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagccaa > 1:4170556/1‑45 (MQ=255) gcgTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTAt < 1:2253670/55‑1 (MQ=255) gcgTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcggcg > 1:1445627/1‑70 (MQ=255) cgTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGa < 1:3839467/71‑1 (MQ=255) tCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAag < 1:2050216/71‑1 (MQ=255) cacaTGAACAGCACTTCTTATTTCTATAACCACTccag < 1:5281818/38‑1 (MQ=255) acaTGAACAGCACTTCTTATTTCTATAACCACTccagcca > 1:5628058/1‑40 (MQ=255) acaTGAACAGCACTTCTTATTTCTATAACCACTccagcca > 1:547798/1‑40 (MQ=255) acaTGAACAGCACTTCTTATTTCTATAACCACTccagcca > 1:1177539/1‑40 (MQ=255) acaTGAACAGCACTTCTTATTTCTATAACCACTccagcca > 1:5499084/1‑40 (MQ=255) acaTGAACAGCACTTCTTATTTCTATAACCACTccagcca > 1:1170920/1‑40 (MQ=255) acaTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAc > 1:3051946/1‑55 (MQ=255) acaTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGaa > 1:5126154/1‑68 (MQ=255) acaTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGaa > 1:2999371/1‑68 (MQ=255) caTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcg < 1:3812713/61‑1 (MQ=255) caTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGt < 1:3012132/71‑1 (MQ=255) aaTGAACAGCACCTCGTTTTTCTACAATCATTccagcca > 1:2136937/2‑39 (MQ=12) aTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGtt < 1:1978814/71‑1 (MQ=255) aTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCCTTATGaa < 1:657668/51‑1 (MQ=255) aCAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTg < 1:1617511/71‑1 (MQ=255) aGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTc < 1:2841665/71‑1 (MQ=255) gCACTTCTTATTTCTATAACCACTACAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGtt > 1:4711858/1‑69 (MQ=255) aCTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCg > 1:3057029/1‑46 (MQ=255) cttcttATTTCTATAACCACTCCAGCCAGTGGCGt < 1:3248070/35‑1 (MQ=255) ttcttATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGa > 1:1530185/1‑71 (MQ=255) tcttATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAag < 1:113318/55‑1 (MQ=255) tcttATTTCTATAACCACTCCAGCCAGTGGCGATATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGAt > 1:2557337/1‑71 (MQ=255) tttCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCgg < 1:2378010/71‑1 (MQ=255) ttCTATAACCACTCCAGCAAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTAGCTGTCACCg > 1:2553774/1‑63 (MQ=255) tCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcggc > 1:3386911/1‑43 (MQ=255) tCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAaga > 1:2577421/1‑49 (MQ=255) tCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGc > 1:3410386/1‑54 (MQ=255) tCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCAcc > 1:105985/1‑61 (MQ=255) tCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGa < 1:917333/70‑1 (MQ=255) cTATAACCACTCCAGCCAGTGGCGTTATGAAACCGt < 1:1583002/36‑1 (MQ=255) cTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATgg < 1:3805493/65‑1 (MQ=255) tataACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATgg < 1:2855517/64‑1 (MQ=255) tataACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATgg < 1:2848725/64‑1 (MQ=255) taACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCTGACa < 1:375061/68‑1 (MQ=255) | CGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACA > minE/833323‑833448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |