| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 106,012 | C→G | 15.7% | A94P (GCC→CCC) ‡ | aroP ← | aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 106,012 | 0 | C | G | 15.7% | 137.9 / 15.5 | 57 | A94P (GCC→CCC) ‡ | aroP | aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base C (28/20); new base G (2/7); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
TTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGAC > minE/105944‑106081 | tttACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGa < 1:2831510/71‑1 (MQ=255) ggCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaa < 1:183838/71‑1 (MQ=255) gCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCg > 1:2009484/1‑66 (MQ=255) cAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTATCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaaaa > 1:244246/1‑71 (MQ=255) cAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaaaa > 1:3502966/1‑71 (MQ=255) cAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaaaa > 1:3464949/1‑71 (MQ=255) cAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaaaa > 1:3340757/1‑71 (MQ=255) cAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaaaa > 1:2890682/1‑71 (MQ=255) ggTCAGCTCTGCCATTCCCACCAGCACGAACATTACCCAGTAGTTCCAGCCAGAGAGGAAGCCCGCaaacg < 1:1526286/70‑3 (MQ=255) gTCAGCTCTGCCATTCCCACCAGCACGAACATTACCCAGTAGTTCCAGCCAGAGAGGAAGCCCGCaaacgg > 1:3006078/1‑68 (MQ=255) gTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGc < 1:3549408/65‑1 (MQ=255) cagctcagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGc > 1:1838204/1‑54 (MQ=255) cagctcagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTg < 1:1434863/70‑1 (MQ=255) agctcagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGcc > 1:2651705/1‑71 (MQ=255) gctcagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaaaa > 1:489770/1‑65 (MQ=255) gctcagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaaaa > 1:1616064/1‑65 (MQ=255) tcagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGa < 1:1497002/52‑1 (MQ=255) agcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAg < 1:2289323/70‑1 (MQ=255) aTGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTAttt < 1:1976010/71‑1 (MQ=255) gCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTtat > 1:1507965/1‑70 (MQ=255) aaCTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAg > 1:725031/1‑71 (MQ=255) cTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGc > 1:4870818/1‑70 (MQ=255) cTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGc > 1:4028542/1‑70 (MQ=255) tAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAAc < 1:1885376/41‑1 (MQ=255) tAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAAc < 1:64571/41‑1 (MQ=255) tAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCaa < 1:3970643/47‑1 (MQ=255) tAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCa > 1:4298674/1‑70 (MQ=255) caCGAACATTACCCAGTAGTTCCAGCCAGAGAGGAAGCCCGCa < 1:1986627/42‑1 (MQ=16) caCGAACATTACCCAGTAGTTCCAGCCAGAGAGGAAGCCCGCa < 1:2035627/42‑1 (MQ=16) aaCGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGt < 1:1876714/71‑1 (MQ=255) aCGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTg > 1:2065810/1‑71 (MQ=255) aCGAACATTACCCAGTAGTTCCAGCCAGAGAGGAAGCCCGCAAACGGTCCCCAGTATTTATAGGCaa < 1:4883786/67‑1 (MQ=255) cGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGccc < 1:1543649/50‑1 (MQ=255) aCATTACCCAGTAGTTCCAGCCAGAGAGGAAGCCCGCAAACGGTCCCCAGTATTTATAGGCAAAGTgggca < 1:1351023/71‑4 (MQ=255) aCAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCa < 1:1130490/62‑1 (MQ=255) aCAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCa < 1:5635643/62‑1 (MQ=255) cAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTaa < 1:4489955/71‑1 (MQ=255) attACCCAGTAGTTCCAGCCAGAGAGGAAGCCCGCAAACGGTCCCCAGTATTtat < 1:336187/53‑1 (MQ=255) gTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAg < 1:2718070/71‑1 (MQ=255) aCCCAGTAGTTCCAGCCAGAGAGGAAGCCCGCAAACGGTCCCCAGTATTta > 1:3805356/1‑51 (MQ=255) cccAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAg > 1:4906245/1‑59 (MQ=255) cccAGTAGTTCCAGCCAGAGAGGAAGCCCGCAAACGGTCCCCAGTATTTATAGGCa < 1:4990146/56‑1 (MQ=255) cAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGcc > 1:2739150/1‑71 (MQ=255) agtagtTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAg > 1:3061920/1‑56 (MQ=255) ttCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCa < 1:1213210/48‑1 (MQ=255) ttCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGAc < 1:3509339/71‑1 (MQ=255) ttCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGAc < 1:361639/71‑1 (MQ=255) tCCAGCCAGAGGCGAAACCGGCTAAACTGCCCCAGTATTTATAAGCAAAGTGGCTaaa > 1:2531373/1‑58 (MQ=255) ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTaaa > 1:5387778/1‑57 (MQ=255) ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTaaa > 1:1183856/1‑57 (MQ=255) ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg > 1:4309736/1‑71 (MQ=255) ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg > 1:1694569/1‑71 (MQ=255) ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg > 1:994333/1‑71 (MQ=255) cagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGttct > 1:1899357/1‑71 (MQ=255) agagGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAg > 1:963747/1‑43 (MQ=255) gagGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTa < 1:3860395/48‑1 (MQ=255) gCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGac > 1:4109909/1‑71 (MQ=255) gCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGac > 1:2519690/1‑71 (MQ=255) | TTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGAC > minE/105944‑106081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |