Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 833,876 C→T 13.4% S778S (AGC→AGT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE833,8760CT13.4% 165.9 / 14.7 67S778S (AGC→AGTnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (37/21);  new base T (4/5);  total (41/26)
Fisher's exact test for biased strand distribution p-value = 2.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

AATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCG  >  minE/833820‑833945
                                                        |                                                                     
aaTGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTc                                                         >  1:1998311/1‑71 (MQ=255)
 aTGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTcc                                                        <  1:5286047/71‑1 (MQ=255)
   ggTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGc                                                                       >  1:750484/1‑54 (MQ=255)
    gTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGAc                                                     <  1:4024818/71‑1 (MQ=255)
      attgAAGGCAAGCTCGACCTGCTGGTGACGCTCGACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGatat                                                   <  1:2025996/68‑1 (MQ=255)
       tGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCg                                                       <  1:2182199/66‑1 (MQ=255)
        ggAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGc                                                                       <  1:311481/49‑1 (MQ=255)
        ggAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGc                                                                       <  1:4466279/49‑1 (MQ=255)
        ggAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGAcatca                                                 >  1:5251164/1‑71 (MQ=255)
         gAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGa                                                      >  1:5042738/1‑65 (MQ=255)
         gAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGAcatcat                                                >  1:5199391/1‑71 (MQ=255)
         gAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGAcatca                                                 <  1:1612153/70‑1 (MQ=255)
         gAAGGCAAGCTCGACCTGCTGGTGACGCTCGACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGAtatcg                                                 <  1:4205290/70‑2 (MQ=255)
          aaGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCAtt                                               >  1:2428842/1‑71 (MQ=255)
             gCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTg                                            <  1:5113514/71‑1 (MQ=255)
              cAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCAc                                                                     >  1:796553/1‑45 (MQ=255)
                aGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACGTGTCTCTATTCCGACATCAttt                                              >  1:4358453/1‑66 (MQ=255)
                aGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGcc                                          >  1:4173647/1‑70 (MQ=255)
                 gCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGAcatca                                                 >  1:110470/1‑62 (MQ=255)
                 gCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGa                                        >  1:5493622/1‑71 (MQ=255)
                 gCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGa                                        <  1:4925303/71‑1 (MQ=255)
                    ggATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTACGAcatca                                                 >  1:5185177/1‑59 (MQ=255)
                     gATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGc                                    <  1:5279210/71‑1 (MQ=255)
                     gATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGc                                    <  1:2478558/71‑1 (MQ=255)
                      aTCTGGAGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGc                                    >  1:728416/1‑70 (MQ=255)
                        cTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGa                                                      >  1:785298/1‑50 (MQ=255)
                        cTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGa                                                      >  1:1669180/1‑50 (MQ=255)
                           gtggtTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGActt                               <  1:3872112/70‑3 (MQ=255)
                              gtTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCAtt                                               <  1:983705/51‑1 (MQ=255)
                              gtTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCAtt                                               <  1:3865070/51‑1 (MQ=255)
                               gtACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGcc                                          <  1:3076230/54‑1 (MQ=255)
                                 aCGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACg                         <  1:4562829/70‑1 (MQ=255)
                                 aCGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACg                         <  1:1473821/70‑1 (MQ=255)
                                 aCGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACg                         >  1:2414707/1‑70 (MQ=255)
                                 aCGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACg                         <  1:2530617/70‑1 (MQ=255)
                                  cGCTCGACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGaa                       <  1:774887/71‑1 (MQ=255)
                                   gCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaaa                      <  1:5376024/71‑1 (MQ=255)
                                   gCTCGACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGaaa                      <  1:3798898/71‑1 (MQ=255)
                                     tcgACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGaaaa                     >  1:1865983/3‑70 (MQ=255)
                                      cgACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGaa                       >  1:2054885/2‑67 (MQ=255)
                                       gACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaaaaa                    <  1:227333/69‑1 (MQ=255)
                                       gACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaaaaa                    <  1:2869982/69‑1 (MQ=255)
                                       gACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGaaaaa                    <  1:2516655/69‑1 (MQ=255)
                                       gACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAga                  >  1:3490634/1‑71 (MQ=255)
                                       gACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAga                  >  1:3497426/1‑71 (MQ=255)
                                        aCTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGcc                                          >  1:3902865/1‑46 (MQ=255)
                                          ttCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAgacga               >  1:2829301/1‑71 (MQ=255)
                                            ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGAc                                 >  1:4541119/1‑51 (MQ=255)
                                            ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa             >  1:5451873/1‑71 (MQ=255)
                                            ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa             >  1:3333857/1‑71 (MQ=255)
                                            ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa             >  1:1729023/1‑71 (MQ=255)
                                            ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa             >  1:252478/1‑71 (MQ=255)
                                            ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa             >  1:3799207/1‑71 (MQ=255)
                                            ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa             >  1:3290762/1‑71 (MQ=255)
                                            ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGCCGACCTGGTACGAAAAAGACGACa             >  1:3451766/1‑71 (MQ=255)
                                            ccGTCTCTCGAGCACCTGTCTCTATTCCGAcatca                                                 >  1:4583525/1‑35 (MQ=255)
                                             cGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaaa                      >  1:4675448/1‑61 (MQ=255)
                                             cGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa             >  1:590478/1‑70 (MQ=255)
                                              gTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaag                      >  1:5404182/1‑59 (MQ=255)
                                              gTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaaaa                     >  1:1978027/1‑61 (MQ=255)
                                                   tCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGAc                                 >  1:2661012/1‑44 (MQ=255)
                                                     gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGc                                    <  1:4672097/39‑1 (MQ=255)
                                                     gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGc                                    <  1:3008983/39‑1 (MQ=255)
                                                     gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGc                                    <  1:990790/39‑1 (MQ=255)
                                                     gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa             >  1:2271004/1‑62 (MQ=255)
                                                     gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa             >  1:460371/1‑62 (MQ=255)
                                                     gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa             >  1:4517095/1‑62 (MQ=255)
                                                     gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa             >  1:3676956/1‑62 (MQ=255)
                                                      aGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTc   >  1:5319269/1‑71 (MQ=255)
                                                       gCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCg  >  1:2561185/1‑71 (MQ=255)
                                                        |                                                                     
AATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCG  >  minE/833820‑833945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: