| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 833,909 | G→C | 29.0% | T789T (ACG→ACC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 833,909 | 0 | G | C | 29.0% | 127.0 / 61.1 | 86 | T789T (ACG→ACC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (36/25); new base C (17/8); total (53/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCG > minE/833841‑833978 | gATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGc < 1:2478558/71‑1 (MQ=255)gATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGc < 1:5279210/71‑1 (MQ=255) aTCTGGAGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGc > 1:728416/1‑70 (MQ=255) gtggtTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGActt < 1:3872112/70‑3 (MQ=255) aCGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACg < 1:2530617/70‑1 (MQ=255) aCGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACg < 1:1473821/70‑1 (MQ=255) aCGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACg > 1:2414707/1‑70 (MQ=255) aCGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACg < 1:4562829/70‑1 (MQ=255) cGCTCGACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGaa < 1:774887/71‑1 (MQ=255) gCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaaa < 1:5376024/71‑1 (MQ=255) gCTCGACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGaaa < 1:3798898/71‑1 (MQ=255) tcgACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGaaaa > 1:1865983/3‑70 (MQ=255) cgACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGaa > 1:2054885/2‑67 (MQ=255) gACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaaaaa < 1:2869982/69‑1 (MQ=255) gACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaaaaa < 1:227333/69‑1 (MQ=255) gACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGaaaaa < 1:2516655/69‑1 (MQ=255) gACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAga > 1:3497426/1‑71 (MQ=255) gACTTCCGCATGTCCAGTACCTGCCTGTTCTCCGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAga > 1:3490634/1‑71 (MQ=255) ttCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAgacga > 1:2829301/1‑71 (MQ=255) ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGAc > 1:4541119/1‑51 (MQ=255) ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:1729023/1‑71 (MQ=255) ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:3290762/1‑71 (MQ=255) ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:3333857/1‑71 (MQ=255) ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:252478/1‑71 (MQ=255) ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:5451873/1‑71 (MQ=255) ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:3799207/1‑71 (MQ=255) ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGCCGACCTGGTACGAAAAAGACGACa > 1:3451766/1‑71 (MQ=255) cGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaaa > 1:4675448/1‑61 (MQ=255) cGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:590478/1‑70 (MQ=255) gTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaag > 1:5404182/1‑59 (MQ=255) gTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaaaa > 1:1978027/1‑61 (MQ=255) tCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGAc > 1:2661012/1‑44 (MQ=255) gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGc < 1:4672097/39‑1 (MQ=255) gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGc < 1:990790/39‑1 (MQ=255) gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGc < 1:3008983/39‑1 (MQ=255) gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:4517095/1‑62 (MQ=255) gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:460371/1‑62 (MQ=255) gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:2271004/1‑62 (MQ=255) gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:3676956/1‑62 (MQ=255) aGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTc > 1:5319269/1‑71 (MQ=255) gCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCg > 1:2561185/1‑71 (MQ=255) tGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaaaaa < 1:5502763/48‑1 (MQ=255) gTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATg > 1:5295264/1‑71 (MQ=255) tctcTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGaaa < 1:4890858/44‑1 (MQ=255) tctcTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATAc > 1:4163088/1‑60 (MQ=255) tctcTATTCCGACATCATTTTGCCGACCGCGACCTg > 1:2024091/1‑36 (MQ=255) tATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCa < 1:2424132/68‑1 (MQ=255) tATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATcc > 1:2999031/1‑71 (MQ=255) ttCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCg < 1:3539171/70‑1 (MQ=255) ctCCGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAg < 1:541957/40‑1 (MQ=12) ctCCGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGaa > 1:1923086/2‑51 (MQ=255) ctCCGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGt > 1:141150/2‑71 (MQ=255) tCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGc > 1:983598/1‑64 (MQ=255) tCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGc > 1:3065344/1‑64 (MQ=255) ccGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAgac > 1:3400195/1‑41 (MQ=16) ccGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGaa > 1:1487850/1‑49 (MQ=255) ccGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGaa > 1:2381677/1‑49 (MQ=255) ccGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt < 1:5072479/70‑1 (MQ=255) cGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAgac < 1:416510/40‑1 (MQ=16) cGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAgac < 1:384137/40‑1 (MQ=16) cGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATg < 1:4124788/46‑1 (MQ=255) cGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa > 1:4247396/1‑71 (MQ=255) cGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa > 1:2054840/1‑71 (MQ=255) cGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTa > 1:2743258/1‑71 (MQ=255) gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa < 1:1043388/67‑1 (MQ=255) gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTAt > 1:3568848/4‑71 (MQ=255) gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTAt > 1:3897985/4‑71 (MQ=255) gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTAt > 1:892632/4‑71 (MQ=255) gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTAt > 1:892630/4‑71 (MQ=255) aCATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATa > 1:1793678/1‑48 (MQ=255) aCATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTa > 1:426848/1‑69 (MQ=255) catcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTc < 1:5664120/71‑1 (MQ=255) atcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGata > 1:3466423/1‑55 (MQ=255) atcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGata > 1:4274569/1‑55 (MQ=255) atcgtTCTGCCCACCGCCACCTGCTACGAAAAAGACGATATGaa > 1:4673438/1‑44 (MQ=12) tcatTTTGCCGACGGCGACCTGGTACGAAAAAgac < 1:1829124/35‑1 (MQ=255) catTTTGCCGACGGCGACCTGGTACGAAAAAGAAGACATGAATACTTCGGATATGCATCCGTTTATTCAcc > 1:2473935/1‑71 (MQ=255) caatttGCCGACGGCGACCTGGTACGAAAAAGACTACATGAATACTTCGGATATGCATCCGTTTATTCAcc > 1:1915154/4‑71 (MQ=255) atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCg < 1:1370847/48‑1 (MQ=255) atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGa < 1:4351762/50‑1 (MQ=255) atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAccc > 1:1279436/1‑71 (MQ=255) tttGCCGACGGCGACCTGGTACGAAAAAGACGACATGaa < 1:4593526/39‑1 (MQ=255) gCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTg < 1:3934286/70‑1 (MQ=255) ccGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGata < 1:269858/46‑1 (MQ=255) aCGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGata < 1:2219525/43‑1 (MQ=255) cGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcg > 1:182574/1‑71 (MQ=255) ggCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt < 1:3505476/51‑1 (MQ=255) | GATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCG > minE/833841‑833978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |