| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 833,933 | C→T | 32.7% | D797D (GAC→GAT) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 833,933 | 0 | C | T | 32.7% | 124.0 / 80.3 | 101 | D797D (GAC→GAT) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (36/32); new base T (20/13); total (56/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 110 total aligned reads displayed.
CCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAG > minE/833864‑834000 | ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:3799207/1‑71 (MQ=255)ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:3333857/1‑71 (MQ=255)ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:3290762/1‑71 (MQ=255)ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:1729023/1‑71 (MQ=255)ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:5451873/1‑71 (MQ=255)ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGCCGACCTGGTACGAAAAAGACGACa > 1:3451766/1‑71 (MQ=255) cGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:590478/1‑70 (MQ=255) gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:3676956/1‑62 (MQ=255) gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:2271004/1‑62 (MQ=255) gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:460371/1‑62 (MQ=255) gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACa > 1:4517095/1‑62 (MQ=255) aGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTc > 1:5319269/1‑71 (MQ=255) gCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCg > 1:2561185/1‑71 (MQ=255) gTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATg > 1:5295264/1‑71 (MQ=255) tctcTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATAc > 1:4163088/1‑60 (MQ=255) tATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCa < 1:2424132/68‑1 (MQ=255) tATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATcc > 1:2999031/1‑71 (MQ=255) ttCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCg < 1:3539171/70‑1 (MQ=255) ctCCGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGaa > 1:1923086/2‑51 (MQ=255) ctCCGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGt > 1:141150/2‑71 (MQ=255) tCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGc > 1:3065344/1‑64 (MQ=255) ccGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGaa > 1:2381677/1‑49 (MQ=255) ccGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt < 1:5072479/70‑1 (MQ=255) cGATATCGTTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa > 1:4247396/1‑71 (MQ=255) cGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTa > 1:2743258/1‑71 (MQ=255) gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTa < 1:1043388/67‑1 (MQ=255) gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTAt > 1:892632/4‑71 (MQ=255) gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTAt > 1:892630/4‑71 (MQ=255) gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTAt > 1:3568848/4‑71 (MQ=255) gatatcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTAt > 1:3897985/4‑71 (MQ=255) aCATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATa > 1:1793678/1‑48 (MQ=255) aCATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTa > 1:426848/1‑69 (MQ=255) atcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGata > 1:4274569/1‑55 (MQ=255) atcgtTCTGCCCACCGCCACCTGGTACGAAAAAGACGATATGAACACCTCGGata > 1:3466423/1‑55 (MQ=255) atcgtTCTGCCCACCGCCACCTGCTACGAAAAAGACGATATGaa > 1:4673438/1‑44 (MQ=12) catTTTGCCGACGGCGACCTGGTACGAAAAAGAAGACATGAATACTTCGGATATGCATCCGTTTATTCAcc > 1:2473935/1‑71 (MQ=255) caatttGCCGACGGCGACCTGGTACGAAAAAGACTACATGAATACTTCGGATATGCATCCGTTTATTCAcc > 1:1915154/4‑71 (MQ=255) atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCg < 1:1370847/48‑1 (MQ=255) atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGa < 1:4351762/50‑1 (MQ=255) atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAccc > 1:1279436/1‑71 (MQ=255) tttGCCGACGGCGACCTGGTACGAAAAAGACGACATGaa < 1:4593526/39‑1 (MQ=255) gCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTg < 1:3934286/70‑1 (MQ=255) ccGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGata < 1:269858/46‑1 (MQ=255) aCGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGata < 1:2219525/43‑1 (MQ=255) cGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcg > 1:182574/1‑71 (MQ=255) tgCGACGTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt < 1:5074128/50‑1 (MQ=255) ggCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt < 1:3505476/51‑1 (MQ=255) gCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcggc < 1:4778080/71‑1 (MQ=255) gCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcggc < 1:2071345/71‑1 (MQ=255) cGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTg < 1:1610348/62‑1 (MQ=255) gACCTGGTACGAAAAAGACGACATGAATACTTCGGata < 1:1009580/38‑1 (MQ=255) gACCTGGTACGAAAAAGACGACATGAATACTTCGGata < 1:3961289/38‑1 (MQ=255) ccTGGTACGAAAAAGACGACATGAATACTTCGGata > 1:1804533/1‑36 (MQ=255) cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGtt < 1:3418381/45‑1 (MQ=255) cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGtt < 1:1536267/45‑1 (MQ=255) cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGtt < 1:1961068/45‑1 (MQ=255) cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGtt < 1:2369790/45‑1 (MQ=255) cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTc > 1:3764463/1‑70 (MQ=255) cTGGTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCg < 1:495935/71‑1 (MQ=255) tGGTACGAAAAAGACGATATGAACACCTCGGATATGc > 1:4270774/1‑37 (MQ=25) tGGTACGAAAAAGACGATATGAACACCTCGGATATGCa > 1:705505/1‑38 (MQ=25) tGGTACGAAAAAGACGATATGAACACCTCGGATATGCa > 1:2451133/1‑38 (MQ=25) tGGTACGAAAAAGACGATATGAACACCTCGGATATGCa > 1:2547601/1‑38 (MQ=25) gTACGAAAAAGACTATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGa < 1:2753154/69‑1 (MQ=255) gTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCttt > 1:2928721/1‑54 (MQ=255) gTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGa < 1:774894/69‑1 (MQ=255) gTACGAAAAAGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATc > 1:5188932/1‑71 (MQ=255) gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGt < 1:34185/41‑1 (MQ=255) gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCTGTCGAt < 1:5336978/70‑1 (MQ=255) gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGAt < 1:2241915/70‑1 (MQ=255) gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGAt < 1:4590972/70‑1 (MQ=255) gTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGAt < 1:3132333/70‑1 (MQ=255) tACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCt < 1:1476946/53‑1 (MQ=255) tACGAAAAAGACGACATAAATACTTCGGATATGCATCCGt < 1:4233956/40‑1 (MQ=255) aCGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCg > 1:93619/1‑71 (MQ=255) cGAAAAAGACGACATGAATACTTCGGATATGCATCCGtt < 1:5180870/39‑1 (MQ=255) gAAAAAGACGATATGAACACCTCGGATATGCATCCGtt < 1:3706483/38‑1 (MQ=37) gAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTa < 1:416101/40‑1 (MQ=255) gAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCAcc < 1:533759/46‑1 (MQ=255) aaaaaGACGACATGAATACTTCGGATATGCATCCGtt > 1:59931/1‑37 (MQ=255) aaaaaGACGACATGAATACTTCGGATATGCATCCGtt > 1:2255891/1‑37 (MQ=255) aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCa < 1:1322700/43‑1 (MQ=255) aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCa < 1:1377918/43‑1 (MQ=255) aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGcc > 1:159557/1‑71 (MQ=255) aaaaGACGATATGAACATCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGcgt < 1:5196116/71‑3 (MQ=255) aaaaGACGATATGAACACCTCGGATATGCATCCGTTTa > 1:3362053/1‑38 (MQ=37) aaaaGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGcgt < 1:1165135/71‑3 (MQ=255) aaaaGACGACATGAATACTTCGGATATGCATCCGttt > 1:4894266/1‑37 (MQ=255) aaaaGACGACATGAATACTTCGGATATGCATCCGttt > 1:772551/1‑37 (MQ=255) aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGcc < 1:2971644/70‑1 (MQ=255) aaaGACGATATGAACACCTCGGATATGCATCCGTTTATTCATCCGCTTTCTGCGGCGGTCGATCCGGCGTg < 1:527492/71‑1 (MQ=255) acgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTgcggc < 1:4214709/52‑1 (MQ=255) cgatATGAACACCTCGGATATGCATCCGTTTATTCATCCGCttt > 1:2625660/1‑42 (MQ=25) cgatATGAACACCTCGGATATGCATCCGTTTATTCATCCGCttt > 1:796807/1‑42 (MQ=25) cgatATGAACACCTCGGATATGCATCCGTTTATTCATCCGCttt > 1:1265174/1‑42 (MQ=25) gacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAg < 1:5605469/70‑1 (MQ=255) gacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAg < 1:673802/70‑1 (MQ=255) gacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAg < 1:2757281/70‑1 (MQ=255) acATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGa < 1:295201/55‑1 (MQ=255) cATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTg > 1:4451928/1‑40 (MQ=255) | CCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAG > minE/833864‑834000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |