| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,272 | C→T | 24.1% | I910I (ATC→ATT) ‡ | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,272 | 0 | C | T | 24.1% | 149.5 / 43.5 | 83 | I910I (ATC→ATT) ‡ | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (21/41); new base T (10/10); total (32/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTA > minE/834204‑834340 | gTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCgg < 1:2863443/71‑1 (MQ=255)gTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCgg < 1:512139/71‑1 (MQ=255) tCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGGGAAAATCGGt < 1:918393/71‑1 (MQ=255) tCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGt < 1:4618116/71‑1 (MQ=255) tCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGt < 1:2213556/71‑1 (MQ=255) gTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTaa > 1:2423121/1‑71 (MQ=255) agagCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATg > 1:1718139/1‑71 (MQ=255) agagCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATg > 1:2292926/1‑71 (MQ=255) gagCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATgg < 1:2770130/71‑1 (MQ=255) gagCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATgg < 1:2331647/71‑1 (MQ=255) agCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGc < 1:2732854/71‑1 (MQ=255) cgATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGt > 1:2191912/1‑70 (MQ=255) aTTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCgg < 1:3378964/58‑1 (MQ=255) ttATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTaa > 1:5053776/1‑60 (MQ=255) ttATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGt > 1:2175296/1‑67 (MQ=255) ttATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAg > 1:4055910/1‑71 (MQ=255) aTCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAgg < 1:3738083/70‑1 (MQ=255) cGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAgg < 1:4685043/67‑1 (MQ=255) ggCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTg < 1:2770132/71‑1 (MQ=255) aCTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGG‑AAAGGGATTGCCTgg < 1:419840/71‑1 (MQ=255) ttACAAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAggg < 1:3928344/61‑1 (MQ=255) aCGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGaa < 1:291860/70‑1 (MQ=255) tgAACGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAAt < 1:271263/45‑1 (MQ=255) aaCGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTgg < 1:267508/65‑1 (MQ=255) aaCGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTGGAATAcc > 1:4518783/1‑71 (MQ=255) cGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGt < 1:1646611/39‑1 (MQ=255) cGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGt < 1:3049915/39‑1 (MQ=255) cGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAggg < 1:584154/54‑1 (MQ=255) gCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAGGGa < 1:2753986/54‑1 (MQ=255) gCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAGGGa < 1:2556674/54‑1 (MQ=255) gCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTgg < 1:3178416/62‑1 (MQ=255) cTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTaa > 1:4420579/1‑39 (MQ=255) cTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTaa > 1:3443090/1‑39 (MQ=255) cTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTaa > 1:2996780/1‑39 (MQ=255) cTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGCTAATGGCGGTAAAGGGATTGCCTGGAACACCCaga < 1:761213/71‑1 (MQ=255) tttACCTCTATCGGCCCGCGGATGGAGAAAATCGGTa > 1:2821064/1‑37 (MQ=255) ttACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGc < 1:2732616/55‑1 (MQ=255) ttACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGc < 1:1780629/55‑1 (MQ=255) tACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTa > 1:2957626/1‑44 (MQ=255) tACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGc < 1:3115838/70‑1 (MQ=255) tACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTGGAATACCCaggat < 1:4847850/70‑4 (MQ=255) aCCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCg < 1:5329022/70‑1 (MQ=255) ccTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCga < 1:2660199/70‑1 (MQ=255) ccTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCga < 1:3160474/70‑1 (MQ=255) ctctATCGGCCCGCTGATGGAGAAAATCGGTAATgg < 1:2364127/36‑1 (MQ=255) ctctATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCgaga < 1:4396547/71‑1 (MQ=255) ctATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGc < 1:210801/65‑1 (MQ=255) tATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATg > 1:1766222/1‑70 (MQ=255) aTCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGa > 1:894272/1‑71 (MQ=255) aTCGGCCCGCTGATGGAGAAAATAGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGa > 1:4372801/1‑71 (MQ=255) tCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTgg < 1:4481599/50‑1 (MQ=255) tCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGAt > 1:3797728/1‑71 (MQ=255) cGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATgg < 1:802142/68‑1 (MQ=255) cGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTgg < 1:2845189/44‑1 (MQ=255) cGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTgg < 1:4663557/44‑1 (MQ=255) cGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTgg < 1:2551366/44‑1 (MQ=255) cGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCga < 1:3581008/58‑1 (MQ=255) cGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCga < 1:5435845/58‑1 (MQ=255) gCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTgg < 1:1579095/43‑1 (MQ=255) cTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTgt < 1:4322964/42‑2 (MQ=255) gATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTGGaa > 1:5430540/1‑42 (MQ=16) gATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTGGaa > 1:2891107/1‑42 (MQ=16) gATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTGGaa > 1:5658164/1‑42 (MQ=16) gATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTGGaa > 1:935884/1‑42 (MQ=16) gATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTGGa > 1:1536115/1‑41 (MQ=16) gATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTGGa > 1:198260/1‑41 (MQ=16) gATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTGGAATAcc > 1:515111/1‑46 (MQ=255) gATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTGGAATAcc > 1:2030043/1‑46 (MQ=255) gATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTGGAATAcc > 1:2966700/1‑46 (MQ=255) tGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACAccc < 1:4887448/45‑1 (MQ=255) tGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACAccc < 1:1775653/45‑1 (MQ=255) tGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACAccc < 1:2677410/45‑1 (MQ=255) agaAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCt > 1:998717/1‑70 (MQ=255) agaAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCt > 1:5344887/1‑70 (MQ=255) gaAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCa < 1:4824569/71‑1 (MQ=255) aaaTCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAAc < 1:205472/71‑1 (MQ=255) aaaTCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAAc < 1:792991/71‑1 (MQ=255) aaaTCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAAc > 1:1411784/1‑71 (MQ=255) aaTCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCgaga > 1:2346196/1‑47 (MQ=255) aaTCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCgaga > 1:3745241/1‑47 (MQ=255) aaTCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCga < 1:3094082/45‑1 (MQ=255) aTCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTa < 1:5024182/71‑1 (MQ=255) aTCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCAAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTa > 1:665690/1‑71 (MQ=255) | GTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTA > minE/834204‑834340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |