| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,404 | G→A | 19.3% | E954E (GAG→GAA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,404 | 0 | G | A | 19.3% | 130.8 / 23.4 | 62 | E954E (GAG→GAA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (33/17); new base A (7/5); total (40/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
AACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCG > minE/834336‑834471 | aaCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAgaga < 1:4914177/70‑1 (MQ=255) aCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatg < 1:961108/71‑1 (MQ=255) aCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatg < 1:2228065/71‑1 (MQ=255) acacCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATc > 1:5476158/1‑71 (MQ=255) cacCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATcc < 1:5451545/71‑1 (MQ=255) acCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCt > 1:3485436/1‑71 (MQ=255) cAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATc > 1:4108804/1‑67 (MQ=255) cAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTg > 1:983200/1‑70 (MQ=255) cAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGa > 1:2804152/1‑71 (MQ=255) aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTg < 1:3280953/67‑1 (MQ=255) aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTg < 1:3049245/67‑1 (MQ=255) aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac > 1:3238812/1‑71 (MQ=255) aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac > 1:3474654/1‑71 (MQ=255) aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac > 1:117271/1‑71 (MQ=255) aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac < 1:799572/71‑1 (MQ=255) aGCGGAAGGTCCGGCGAAAGGACAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac > 1:2200624/1‑71 (MQ=255) cGGAAGGTCCGGGGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGac > 1:4328888/1‑67 (MQ=255) cGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATTATCCTGACACTg < 1:5524038/71‑1 (MQ=255) cGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGac > 1:4090681/1‑67 (MQ=255) gAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGc < 1:3128083/71‑1 (MQ=255) aaGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGaca > 1:4045272/1‑65 (MQ=255) aaGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGac > 1:5615062/1‑64 (MQ=255) ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc > 1:279286/1‑71 (MQ=255) ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc > 1:2593234/1‑71 (MQ=255) ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc > 1:2905575/1‑71 (MQ=255) ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc > 1:1892218/1‑71 (MQ=255) ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc > 1:5262326/1‑71 (MQ=255) cGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa > 1:2987085/1‑70 (MQ=255) aaGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatgat > 1:2462898/1‑46 (MQ=255) aaGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatgat > 1:2982068/1‑46 (MQ=255) aaGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatgat > 1:5111066/1‑46 (MQ=255) aGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACg > 1:1376679/1‑71 (MQ=255) ccAGCCGATGCTGAACACCGCAATTGATGC‑‑CAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAg < 1:482382/71‑1 (MQ=255) aGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAg < 1:2406312/71‑1 (MQ=255) ccGATGATTGACACCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGaaa > 1:246601/1‑58 (MQ=255) tGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTTAGGTAGcc > 1:3078595/1‑71 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa < 1:3195754/51‑1 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa < 1:4031152/51‑1 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa < 1:1163626/51‑1 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa < 1:2659106/51‑1 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa < 1:2746630/51‑1 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACg > 1:5672233/1‑59 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCg > 1:524524/1‑71 (MQ=255) cTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCAGAAACCAACGGTCAGGTAGCCGt > 1:2728700/1‑71 (MQ=255) ttgacacCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGt > 1:3927219/4‑70 (MQ=255) gacacCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGtca > 1:2513408/2‑68 (MQ=255) aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAg > 1:2180490/1‑64 (MQ=255) aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTg > 1:1513117/1‑69 (MQ=255) aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGa > 1:1088208/1‑70 (MQ=255) aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGa > 1:1921058/1‑70 (MQ=255) acacCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAc > 1:3256172/1‑42 (MQ=255) acCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGc < 1:5314949/71‑1 (MQ=255) acCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCACCGGTCAGGTAGCCGTGAAAGc < 1:5325265/71‑1 (MQ=255) acCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGc < 1:2065873/71‑1 (MQ=255) ccGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGcg < 1:2907865/71‑2 (MQ=255) ccGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGcg < 1:4829589/71‑2 (MQ=255) ccGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGcc < 1:2940260/71‑1 (MQ=255) cGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGcg < 1:2075062/70‑2 (MQ=255) ccaTTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGCGTgg > 1:1319085/3‑71 (MQ=255) ccaTTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGCGTgg > 1:4249939/3‑71 (MQ=255) caTTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCaa > 1:2852855/2‑45 (MQ=12) caTTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCaa > 1:3723102/2‑45 (MQ=12) caTTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGCGTggc < 1:2837042/70‑2 (MQ=255) ttGATGCGGCAGAGATGATCCTGACACTGGCACCGGaaa < 1:2889438/39‑1 (MQ=255) tGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTg > 1:5306018/1‑66 (MQ=255) gATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTg > 1:5443950/1‑65 (MQ=255) gATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGc > 1:3230775/1‑71 (MQ=255) gATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGc > 1:4143126/1‑71 (MQ=255) agagATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGc > 1:1048035/1‑70 (MQ=255) agagATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCg < 1:5142443/71‑1 (MQ=255) | AACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCG > minE/834336‑834471 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |