| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,446 | C→A | 23.3% | A968A (GCC→GCA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,446 | 0 | C | A | 23.3% | 141.4 / 26.1 | 64 | A968A (GCC→GCA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (31/18); new base A (10/5); total (41/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAG > minE/834376‑834513 | tGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTTAGGTAGcc > 1:3078595/1‑71 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCg > 1:524524/1‑71 (MQ=255) cTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCAGAAACCAACGGTCAGGTAGCCGt > 1:2728700/1‑71 (MQ=255) ttgacacCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGt > 1:3927219/4‑70 (MQ=255) gacacCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGtca > 1:2513408/2‑68 (MQ=255) aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTg > 1:1513117/1‑69 (MQ=255) aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGa > 1:1921058/1‑70 (MQ=255) aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGa > 1:1088208/1‑70 (MQ=255) acCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGc < 1:5314949/71‑1 (MQ=255) acCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCACCGGTCAGGTAGCCGTGAAAGc < 1:5325265/71‑1 (MQ=255) acCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGc < 1:2065873/71‑1 (MQ=255) ccGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGcg < 1:4829589/71‑2 (MQ=255) ccGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGcg < 1:2907865/71‑2 (MQ=255) ccGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGcc < 1:2940260/71‑1 (MQ=255) cGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGcg < 1:2075062/70‑2 (MQ=255) ccaTTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGCGTgg > 1:4249939/3‑71 (MQ=255) ccaTTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGCGTgg > 1:1319085/3‑71 (MQ=255) caTTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGCGTggc < 1:2837042/70‑2 (MQ=255) tGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTg > 1:5306018/1‑66 (MQ=255) gATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTg > 1:5443950/1‑65 (MQ=255) gATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGc > 1:3230775/1‑71 (MQ=255) gATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGc > 1:4143126/1‑71 (MQ=255) agagATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGc > 1:1048035/1‑70 (MQ=255) agagATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCg < 1:5142443/71‑1 (MQ=255) atgatCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGaa < 1:5637538/69‑1 (MQ=255) gatTCTGGCACTGGCCCCGGAAACCAACGGTCATGTTGCAGTCAAAGCGTggca > 1:4797438/1‑52 (MQ=255) gatTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGCGTggca > 1:1999719/1‑52 (MQ=255) gatTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGCGTgg > 1:3563902/1‑52 (MQ=255) gatCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGaa > 1:5447706/1‑67 (MQ=255) cTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCt < 1:166028/40‑1 (MQ=255) cTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCt < 1:1479846/40‑1 (MQ=255) gtcACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGa > 1:1986110/3‑50 (MQ=255) ggCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGa > 1:2867190/1‑50 (MQ=255) aCCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGt < 1:2993730/64‑1 (MQ=255) cGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTggg > 1:920425/1‑36 (MQ=255) cGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTggg > 1:1487802/1‑36 (MQ=255) cGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTggg > 1:1033794/1‑36 (MQ=255) ggAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACg > 1:1766373/1‑70 (MQ=255) gAAACCAACGGTCATGTTGCAGTCAAAGCGTGGCAGGCGCTGGGCGAGATCACCGGGCGCGAACATAc > 1:3798077/1‑68 (MQ=255) gAAACCAACGGTCATGTTGCAGTCAAAGCGTGGCAGGCGCTGGGCGAGATCACCGGGCGCGAACATACCCa > 1:1453460/1‑71 (MQ=255) gAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGc > 1:976663/1‑70 (MQ=255) gAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCa > 1:139084/1‑71 (MQ=255) gAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCa < 1:1737948/71‑1 (MQ=255) aaaCCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGt < 1:4408041/59‑1 (MQ=255) aaaCCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGt > 1:3198639/1‑59 (MQ=255) aaaCCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTg > 1:518618/1‑60 (MQ=255) aCCAACGGTCATGTTGCAGTCAAAGCGTGGCAGGCGCTGGGCGAGATCACCGGGCGCGAACATACCCATCt > 1:836391/1‑71 (MQ=255) aCCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGa < 1:3742897/59‑1 (MQ=255) aCCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGa < 1:3147622/59‑1 (MQ=255) ccAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCa < 1:4148986/61‑1 (MQ=255) ccAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTg < 1:4765093/71‑1 (MQ=255) cAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGt > 1:2733766/1‑55 (MQ=255) cAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTg < 1:4758660/70‑1 (MQ=255) aCGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAAtt > 1:3574503/1‑43 (MQ=255) aCGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGgcg < 1:1617288/71‑1 (MQ=255) cGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGgcg > 1:1951297/1‑70 (MQ=255) cGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGgcg > 1:1671944/1‑70 (MQ=255) ggTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCa < 1:4297539/56‑1 (MQ=255) gTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTg < 1:1907621/71‑1 (MQ=255) cAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCg > 1:2598438/1‑47 (MQ=255) gTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGCCCATACGCATCTGGCGCTGAATa > 1:2461451/1‑70 (MQ=255) aGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGa > 1:5233561/1‑45 (MQ=255) aGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTg > 1:331218/1‑64 (MQ=255) aGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATa > 1:5537256/1‑68 (MQ=255) gCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAaag > 1:3476574/1‑70 (MQ=255) | TGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAG > minE/834376‑834513 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |