| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 834,611 | C→T | 16.5% | Y1023Y (TAC→TAT) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 834,611 | 0 | C | T | 16.5% | 208.2 / 29.2 | 91 | Y1023Y (TAC→TAT) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (46/30); new base T (8/7); total (54/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
CAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATC > minE/834543‑834681 | cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACaa > 1:913106/1‑71 (MQ=255)cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACa < 1:19916/70‑1 (MQ=255)cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACa < 1:3276936/70‑1 (MQ=255) cacaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGcc > 1:5485810/1‑71 (MQ=255) gcaGCCGCGTAAAATTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGcgg < 1:1426495/70‑3 (MQ=255) acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg > 1:2810182/1‑71 (MQ=255) acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg > 1:3431265/1‑71 (MQ=255) acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg > 1:775879/1‑71 (MQ=255) aGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGt < 1:4438733/71‑1 (MQ=255) aGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGt < 1:3199465/71‑1 (MQ=255) gcAAAATTATCTCCAGCCCGCCCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTacac > 1:1316838/1‑70 (MQ=255) gcAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTacac > 1:4136311/1‑70 (MQ=255) taaaaTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACAcc > 1:2529333/2‑70 (MQ=255) cAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACa > 1:1576026/1‑56 (MQ=255) aaaaTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCggg > 1:2979045/1‑62 (MQ=255) aaaTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAAc > 1:692219/1‑71 (MQ=255) aaTTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACg > 1:5147618/1‑71 (MQ=255) ttATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGtt < 1:4566101/71‑1 (MQ=255) tctcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCAc > 1:422689/1‑71 (MQ=255) tctcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCAc > 1:3109015/1‑71 (MQ=255) cAGCCCGACCTGGTCTGGTCTGGAAGATGAACATGTTTCTTACAACGCCGGTTACACCAACGTTCAc < 1:3874263/67‑1 (MQ=255) cAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAga < 1:3325402/71‑2 (MQ=255) cAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGc > 1:3771642/1‑71 (MQ=255) cAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGc > 1:2279472/1‑71 (MQ=255) cAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAgt > 1:3245122/1‑70 (MQ=255) aGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAgtt < 1:3154329/71‑3 (MQ=255) cGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGa > 1:5564691/1‑64 (MQ=255) aCCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc > 1:3053275/1‑70 (MQ=255) ccTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa < 1:2859978/71‑1 (MQ=255) ctggtccggtCTGGAAAGCTATCACGTCTCCTATAACGCGGGATACACCAACGtt < 1:1773061/55‑1 (MQ=255) ctggtccggtCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGtt < 1:4670662/55‑1 (MQ=255) tggtctggtcTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATg > 1:4345503/1‑71 (MQ=255) gtctggtctgGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTg < 1:4221275/61‑1 (MQ=255) gtctggtctgGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGc > 1:1666562/1‑71 (MQ=255) gtccggtctgGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTgg < 1:4443603/70‑1 (MQ=255) ctggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg > 1:1296049/1‑70 (MQ=255) ccggtctggAAAGAGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGc > 1:2991480/3‑69 (MQ=255) tggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa < 1:997608/64‑1 (MQ=255) tggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa < 1:1082990/64‑1 (MQ=255) tggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa < 1:1249383/64‑1 (MQ=255) tggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa < 1:1256267/64‑1 (MQ=255) ggtctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCAc < 1:4005560/71‑1 (MQ=255) gtctggAAGATGAACACGTTTCTTACAACGCCGGTTaca > 1:4015644/1‑39 (MQ=255) gtctggAAGATGAACACGTTTCTTACAACGCCGGTTaca > 1:3717928/1‑39 (MQ=255) gtctggAAGATGAACACGTTTCTTACAACGCCGGTTa > 1:1806213/1‑37 (MQ=255) gtctggAAGATGAACACGTTTCTTACAACGCCGGTTa > 1:4715456/1‑37 (MQ=255) gtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGt < 1:191628/68‑1 (MQ=255) gtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTAc < 1:3967120/70‑1 (MQ=255) gtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTAc < 1:4367756/70‑1 (MQ=255) gtctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACg > 1:4241321/1‑71 (MQ=255) tctggATGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTg < 1:3257601/55‑1 (MQ=255) ctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGct > 1:2064287/1‑71 (MQ=255) tggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGc < 1:4808438/69‑1 (MQ=255) gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc > 1:5173350/1‑71 (MQ=255) gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc < 1:1764458/71‑1 (MQ=255) gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc < 1:1157887/71‑1 (MQ=255) aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg < 1:1013436/71‑1 (MQ=255) gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc < 1:5523829/52‑1 (MQ=255) tGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctcac > 1:223304/1‑64 (MQ=255) gAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc < 1:4209999/49‑1 (MQ=255) gAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcg > 1:1469327/1‑71 (MQ=255) aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt < 1:2843237/71‑1 (MQ=255) acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa < 1:2550197/49‑1 (MQ=255) acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa < 1:2962516/49‑1 (MQ=255) acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt > 1:4333227/1‑70 (MQ=255) acGTTTCTTACAACGCCGGTTACACCAACGTTCACGAg > 1:4555694/1‑38 (MQ=255) acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAg > 1:5009384/1‑71 (MQ=255) acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAg < 1:266260/71‑1 (MQ=255) acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAg > 1:1235246/1‑71 (MQ=255) cGTTTCTTACAACGCCGGTTACACCAACGTTCACGAg > 1:178022/1‑37 (MQ=255) cGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAg > 1:1435995/1‑70 (MQ=255) cGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc < 1:2199704/71‑1 (MQ=255) tttCTTACAACGCCGGTTACACCAACTTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc < 1:135404/69‑1 (MQ=255) tttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATgg < 1:3958168/47‑1 (MQ=255) tttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa > 1:538620/1‑71 (MQ=255) ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATc > 1:1707135/1‑40 (MQ=255) ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc > 1:814090/1‑68 (MQ=255) ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc > 1:4392780/1‑68 (MQ=255) ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc > 1:2621404/1‑68 (MQ=255) ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc > 1:2614700/1‑68 (MQ=255) tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa > 1:3060372/1‑42 (MQ=255) tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa > 1:174100/1‑42 (MQ=255) tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGc > 1:5035308/1‑52 (MQ=255) tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt > 1:1481969/1‑71 (MQ=255) cTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgt > 1:2537173/1‑59 (MQ=255) cTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgt > 1:3420004/1‑59 (MQ=255) ttACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg > 1:1369880/1‑56 (MQ=255) ttACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg > 1:2963754/1‑56 (MQ=255) ttACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg > 1:1440838/1‑56 (MQ=255) ttACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg > 1:863518/1‑56 (MQ=255) tACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa < 1:1398375/39‑1 (MQ=255) cAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGc > 1:1838750/1‑41 (MQ=255) cAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGc > 1:2665952/1‑41 (MQ=255) cAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATc < 1:3411846/71‑1 (MQ=255) | CAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATC > minE/834543‑834681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |