| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,892,019:1 | +GC | 100% | intergenic (‑437/+54) | gudP ← / ← ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,892,019 | 1 | . | G | 100.0% | 210.5 / NA | 60 | intergenic (‑437/+54) | gudP/ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (19/41); total (19/41) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | minE | 1,892,019 | 2 | . | C | 100.0% | 208.6 / NA | 60 | intergenic (‑437/+54) | gudP/ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (19/41); total (19/41) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGT > minE/1891953‑1892072 || ggCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct > 1:3243778/1‑67 (MQ=255)ggCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct > 1:2319323/1‑67 (MQ=255)ggCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct > 1:2284766/1‑67 (MQ=255)ggCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagc > 1:1021943/1‑67 (MQ=255) gCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct > 1:3479105/1‑66 (MQ=255) gCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct < 1:4616267/69‑4 (MQ=255) ccTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa < 1:4203993/71‑1 (MQ=255) aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc < 1:596780/70‑1 (MQ=255) aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc < 1:3405244/70‑1 (MQ=255) aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc < 1:2517624/70‑1 (MQ=255) aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc < 1:2674983/70‑1 (MQ=255) aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg > 1:3434423/1‑71 (MQ=255) aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg > 1:2256463/1‑71 (MQ=255) aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg > 1:3115388/1‑71 (MQ=255) aaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCgg < 1:518472/71‑1 (MQ=255) attcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg < 1:4189847/71‑1 (MQ=255) cgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa < 1:1824193/70‑1 (MQ=255) cgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa < 1:1920085/70‑1 (MQ=255) cgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa < 1:2282658/70‑1 (MQ=255) cgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa < 1:1399967/70‑1 (MQ=255) ttcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt > 1:4846794/1‑68 (MQ=255) ttcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt > 1:942911/1‑68 (MQ=255) cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa < 1:2575501/70‑1 (MQ=255) aaaaCGGCATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa > 1:670825/1‑57 (MQ=255) aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCgg > 1:2688104/1‑49 (MQ=255) aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcg > 1:2599723/1‑66 (MQ=255) aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct > 1:4972076/1‑71 (MQ=255) aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc > 1:2932908/1‑70 (MQ=255) aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtctc < 1:1704781/71‑1 (MQ=255) aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtctc < 1:4618371/71‑1 (MQ=255) aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtctc < 1:2011673/71‑1 (MQ=255) aaCGACATTTACCGCTCGCTGAACATATCAGCTACGCACAGAACCg > 1:4619551/1‑46 (MQ=255) aaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCa > 1:52402/1‑71 (MQ=255) cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATc > 1:568446/1‑71 (MQ=255) cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATc < 1:4239018/71‑1 (MQ=255) cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATc > 1:3480084/1‑71 (MQ=255) cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATc < 1:3356810/71‑1 (MQ=255) cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATc < 1:2538763/71‑1 (MQ=255) aCATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCg < 1:1804166/71‑1 (MQ=255) aCATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCg < 1:2513307/71‑1 (MQ=255) tttCCCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt < 1:1816485/55‑1 (MQ=255) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAAc < 1:2441151/37‑1 (MQ=25) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAAc < 1:2076340/37‑1 (MQ=25) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAAc < 1:874808/37‑1 (MQ=25) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAAc < 1:4680449/37‑1 (MQ=25) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt < 1:4529556/55‑1 (MQ=255) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt < 1:2849025/55‑1 (MQ=255) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt < 1:304020/55‑1 (MQ=255) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt < 1:881375/55‑1 (MQ=255) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt < 1:2683892/55‑1 (MQ=255) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt < 1:3688164/55‑1 (MQ=255) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt < 1:2114002/55‑1 (MQ=255) ttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCa > 1:5158971/1‑63 (MQ=255) ttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt > 1:2060506/1‑71 (MQ=39) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa > 1:3274483/1‑49 (MQ=255) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct > 1:3471539/1‑60 (MQ=255) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct > 1:2918064/1‑60 (MQ=255) ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGt < 1:3101642/71‑1 (MQ=255) tcgctGAACATATCAGCTAAGCACAGACCCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGt < 1:2782315/70‑1 (MQ=255) tcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGt < 1:3936276/70‑1 (MQ=255) cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATa < 1:840843/39‑1 (MQ=37) cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATa < 1:1059162/39‑1 (MQ=37) cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATa < 1:1468492/39‑1 (MQ=37) cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATa < 1:4824030/39‑1 (MQ=37) cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATa < 1:4068402/39‑1 (MQ=37) gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt < 1:2766192/45‑1 (MQ=255) || GGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGT > minE/1891953‑1892072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |