| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 108,284 | T→C | 10.7% | S176S (TCT→TCC) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 108,284 | 0 | T | C | 10.7% | 151.6 / 8.8 | 56 | S176S (TCT→TCC) | aceE | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base T (26/24); new base C (3/3); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTATGGGTCTGGGTCCGA > minE/108216‑108354 | cTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTc > 1:3975400/1‑71 (MQ=255) tGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCAccc > 1:2777565/1‑70 (MQ=255) aagAGCAGCTGCTGAACTTCCGCCGCGAAGTCGACGGCAAGGGCCTGTCCTCGTACCCGCACCCGAAACt > 1:1829558/3‑70 (MQ=255) aGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACt < 1:4074345/70‑1 (MQ=255) ggAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTa < 1:2315943/54‑1 (MQ=255) gcagcTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGc > 1:912929/1‑71 (MQ=255) ggATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAAt > 1:4479318/1‑71 (MQ=255) gATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAAtt < 1:5081467/71‑1 (MQ=255) gATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAAtt < 1:2364702/71‑1 (MQ=255) gATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAAt < 1:1249502/70‑1 (MQ=255) gATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAAt < 1:1688861/70‑1 (MQ=255) gATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAAt < 1:2058886/70‑1 (MQ=255) gATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAAt < 1:3102709/70‑1 (MQ=255) aTAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTc > 1:4948167/1‑71 (MQ=255) tAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCt > 1:1417434/1‑71 (MQ=255) tAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCt > 1:1650229/1‑71 (MQ=255) tAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCt > 1:3270176/1‑71 (MQ=255) gaaCTTCCGCCGCGAAGTCGACGGCAAGGGCCTGTCCTCGTACCCGCACCCGAAACTGATGCCGGACTTCt > 1:1308823/2‑71 (MQ=255) gaaCTTCCGCCGCGAAGTCGACGGCAAGGGCCTGTCCTCGTACCCGCACCCGAAACTGATGCCGGACTTCt > 1:1142796/2‑71 (MQ=255) tCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGt < 1:2377000/68‑1 (MQ=255) tCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGt < 1:2446882/68‑1 (MQ=255) aGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTc > 1:3796696/1‑68 (MQ=255) gAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAAt < 1:5028764/55‑1 (MQ=255) gAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAAt < 1:96439/55‑1 (MQ=255) gTTCTCGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGtt < 1:803461/62‑1 (MQ=255) gTTCACGGCAATGGCCTCTCTTCCTATCCGCCCCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGAcc > 1:3341770/1‑69 (MQ=255) gTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGc > 1:226187/1‑46 (MQ=255) gTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGtt < 1:4356506/62‑1 (MQ=255) gTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGtt < 1:1681075/62‑1 (MQ=255) gTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGtt < 1:4049966/62‑1 (MQ=255) gTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGtt < 1:3827363/62‑1 (MQ=255) tCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTccc > 1:544506/1‑63 (MQ=255) tCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTccc > 1:2118353/1‑63 (MQ=255) cACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCg > 1:4633758/1‑67 (MQ=255) cACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGt > 1:4345289/1‑68 (MQ=255) cACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGt > 1:944058/1‑68 (MQ=255) cACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATc > 1:3405465/1‑71 (MQ=255) cACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATc > 1:1188865/1‑71 (MQ=255) cACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATc > 1:1731088/1‑71 (MQ=255) cACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATc > 1:1973453/1‑71 (MQ=255) cACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATc > 1:2543948/1‑71 (MQ=255) aCGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATc < 1:4580337/70‑1 (MQ=255) aCGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATc < 1:4015569/70‑1 (MQ=255) ggCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTAt > 1:901876/1‑71 (MQ=255) ggCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTAt > 1:2389253/1‑71 (MQ=255) aaTGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTAtggg < 1:4713268/71‑1 (MQ=255) tGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGt < 1:2142976/50‑1 (MQ=255) gCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTcc > 1:4759201/1‑51 (MQ=255) gCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTAtgggtctg > 1:3543206/1‑71 (MQ=255) gCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTAtgggtctg > 1:2655520/1‑71 (MQ=255) gCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTAtgggtct < 1:2425248/70‑1 (MQ=255) gCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTAtgggtct < 1:4802160/70‑1 (MQ=255) ccTGTCCTCGTACCCGCACCCGAAACTGATGCCGGACTTCTGGCAGTTCCCGACCGTGTCGAtgggcctgg < 1:5077737/71‑1 (MQ=255) ccTGTCCTCGTACCCGCACCCGAAACTGATGCCGGACTTCTGGCAGTTCCCGACCGTGTCGAtgggcctgg < 1:5996/71‑1 (MQ=255) ccTGTCCTCGTACCCGCACCCGAAACTGATGCCGGACTTCTGGCAGTTCCCGACCGTGTCGAtgggcctgg < 1:928690/71‑1 (MQ=255) ccTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGAcc < 1:1216028/55‑1 (MQ=255) tctctTACTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTAtgggtctgggt > 1:3214/1‑71 (MQ=255) ctctTCCTATCCGCACCCGAAACTGATGCCGGAAt < 1:3545755/35‑1 (MQ=255) ttCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTAtgggtctgggt > 1:274237/1‑67 (MQ=255) ttCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTATGGGTCTGGGTcc < 1:684846/69‑1 (MQ=255) ttCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTATGGGTCTGGGTcc < 1:2824243/69‑1 (MQ=255) ttCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTATGGGTCTGGGTCCGa > 1:542396/1‑71 (MQ=255) | CTGGAAGGTCGTCTGACTCAGGAGCAGCTGGATAACTTCCGTCAGGAAGTTCACGGCAATGGCCTCTCTTCCTATCCGCACCCGAAACTGATGCCGGAATTCTGGCAGTTCCCGACCGTATCTATGGGTCTGGGTCCGA > minE/108216‑108354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |