| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,064,986 | C→T | 7.4% | N409N (AAC→AAT) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,064,986 | 0 | C | T | 7.4% | 288.0 / 9.4 | 94 | N409N (AAC→AAT) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base C (53/33); new base T (4/3); total (58/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
GTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCT > minE/2064918‑2065056 | gTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGTCAAc < 1:2013513/69‑1 (MQ=255)gTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAAc < 1:4406459/69‑1 (MQ=255)gTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAAc < 1:1138291/69‑1 (MQ=255)gTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAAc < 1:3933078/69‑1 (MQ=255) aTTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCg < 1:3020128/70‑1 (MQ=255) aTTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCg < 1:1839200/70‑1 (MQ=255) tttCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGt > 1:1417124/1‑71 (MQ=255) ttCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGt > 1:2054521/1‑70 (MQ=255) ttCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATCCAGGAAGGCAACATCGGt > 1:4196186/1‑70 (MQ=255) tCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCg > 1:1066766/1‑63 (MQ=255) tCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCg > 1:4376074/1‑63 (MQ=255) tCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCg > 1:3201409/1‑63 (MQ=255) tCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCg > 1:5035159/1‑63 (MQ=255) tCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCg > 1:976847/1‑63 (MQ=255) tCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCg > 1:1156612/1‑63 (MQ=255) gtACACCAACCGCGGCCTGCAATTCCTCGACCTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGtgg < 1:1573557/70‑3 (MQ=19) cacCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGAt < 1:2039917/70‑1 (MQ=255) cacCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:1647461/71‑1 (MQ=255) acCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGAt < 1:4790281/69‑1 (MQ=255) acCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaa < 1:4754065/71‑1 (MQ=255) aCCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:3518716/66‑1 (MQ=255) aCCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:1577864/66‑1 (MQ=255) ccGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:3399149/65‑1 (MQ=255) ccGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:2718564/65‑1 (MQ=255) ccGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:2650053/65‑1 (MQ=255) gTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGaaa > 1:1726146/1‑53 (MQ=255) gTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGaaa > 1:2044388/1‑53 (MQ=255) gTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGaaa > 1:2652513/1‑53 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAAt < 1:2571706/71‑1 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAAt < 1:1285275/71‑1 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAAt < 1:2268039/71‑1 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAAt < 1:2272023/71‑1 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAAt < 1:3085226/71‑1 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAAt < 1:1216528/71‑1 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAc > 1:1420719/1‑71 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAc > 1:3951664/1‑71 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAc > 1:1575573/1‑71 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAc > 1:4756893/1‑71 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAc > 1:1623571/1‑71 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAc > 1:1426723/1‑71 (MQ=255) ttGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCtga > 1:1399795/1‑43 (MQ=255) ttGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCtga > 1:627335/1‑43 (MQ=255) ttGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCtga > 1:1012276/1‑43 (MQ=255) tCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGt > 1:2204377/1‑71 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGaaa > 1:1433436/1‑40 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGaaa > 1:3760790/1‑40 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGaaa > 1:4069536/1‑40 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGaaa > 1:3265514/1‑40 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGaaa > 1:1786136/1‑40 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGaaa > 1:2682547/1‑40 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGaaa > 1:2631084/1‑40 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGaaa > 1:4511602/1‑40 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGaaa > 1:2003139/1‑40 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGt > 1:4955241/1‑70 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGt > 1:425082/1‑70 (MQ=255) cTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:924103/55‑1 (MQ=255) cTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:4709308/55‑1 (MQ=255) ttGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTa > 1:5009297/1‑70 (MQ=255) tGACCTGATTCAGGAAGGCAAAATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTAc > 1:1820771/1‑70 (MQ=255) aCCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAg < 1:2093895/71‑1 (MQ=255) ccTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAAt < 1:1237831/53‑1 (MQ=255) ccTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAg < 1:2491365/70‑1 (MQ=255) ccTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATACCGTCGCGGCTATaaat < 1:1108804/71‑3 (MQ=12) cTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGtt < 1:618086/71‑1 (MQ=255) tGATCCAGGAAGGCAATATCGGCCTGATGAAGGCTGTGGACAAGTTCGAATACCGTCGCGGCTATaaat < 1:2569484/69‑3 (MQ=9) gATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGt > 1:2818843/1‑58 (MQ=255) gATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACaa > 1:491083/1‑66 (MQ=255) gATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACaa > 1:2829745/1‑66 (MQ=255) ttCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGt < 1:1495372/59‑1 (MQ=255) ttCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGt < 1:4595197/59‑1 (MQ=255) ttCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTcc < 1:4621225/71‑1 (MQ=255) ttCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTcc < 1:1625803/71‑1 (MQ=255) ttCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTcc < 1:948051/71‑1 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:5027191/1‑40 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:60001/1‑40 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:2681223/1‑40 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:393144/1‑40 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:1587890/1‑40 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:1851902/1‑40 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:4147094/1‑40 (MQ=255) ccAGGAAGGCAATATCGGCCTGCTCAAGGCCGTGGATAAATTCGAATACCGTCGCGGTTATAAATtctcga > 1:2428536/2‑69 (MQ=255) ccAGGAAGGCAATATCGGCCTGCTCAAGGCCGTGGATAAATTCGAATACCGTCGCGGTTATAAATtctcga > 1:3170143/2‑69 (MQ=255) ccAGGAAGGCAATATCGGCCTGCTCAAGGCCGTGGATAAATTCGAATACCGTCGCGGTTATAAATtctcga > 1:3173542/2‑69 (MQ=255) acAGGAAGGCAATATCGGCCTGCTCAAGGCCGTGGATAAATTCGAATACCGTCGCGGt > 1:1878456/2‑58 (MQ=255) gAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACa < 1:371870/58‑1 (MQ=255) gAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACa < 1:2127057/58‑1 (MQ=255) gAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACa < 1:2026326/58‑1 (MQ=255) aaGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACa < 1:3690460/57‑1 (MQ=255) aaGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTAc > 1:2511348/1‑71 (MQ=255) aGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCtg > 1:1743491/1‑68 (MQ=255) aGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTa > 1:1291949/1‑69 (MQ=255) aGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTa > 1:2254085/1‑69 (MQ=255) aGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTa > 1:5043968/1‑69 (MQ=255) aGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTATCCACCTa > 1:1578098/1‑69 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAcc > 1:991692/1‑71 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAcc > 1:4898665/1‑71 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAcc < 1:3107784/71‑1 (MQ=255) cATCGTTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCt < 1:483579/71‑1 (MQ=255) cATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCt < 1:4866391/71‑1 (MQ=255) cATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCt < 1:483580/71‑1 (MQ=255) | GTTATTTCTATCGCTAAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCT > minE/2064918‑2065056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |