| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,068,677 | A→G | 5.3% | N371N (AAT→AAC) | aer ← | fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,068,677 | 0 | A | G | 5.3% | 221.2 / 2.7 | 75 | N371N (AAT→AAC) | aer | fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component |
| Reads supporting (aligned to +/- strand): ref base A (45/26); new base G (2/2); total (47/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
ACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAAT > minE/2068608‑2068739 | aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCa < 1:60279/70‑1 (MQ=255) gCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTc < 1:5172089/71‑1 (MQ=255) gCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTc < 1:1810667/71‑1 (MQ=255) ccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAg < 1:2222441/71‑1 (MQ=255) ccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAg < 1:2172191/71‑1 (MQ=255) ccaccaCTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa < 1:5181832/69‑1 (MQ=255) tGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa < 1:835635/62‑1 (MQ=255) tGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa < 1:143719/62‑1 (MQ=255) tGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa < 1:2484975/62‑1 (MQ=255) gCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata > 1:3676150/1‑63 (MQ=255) gCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata > 1:4451688/1‑63 (MQ=255) gCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata > 1:2576390/1‑63 (MQ=255) cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata > 1:1703148/1‑62 (MQ=255) cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTg < 1:2549873/70‑1 (MQ=255) tttGCCCTGTTCGCCGGCACGCGCCGCTTCCACGGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCaa > 1:273898/1‑71 (MQ=255) tttGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCa > 1:403341/1‑70 (MQ=255) ttGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCaa > 1:1781715/1‑70 (MQ=255) tGTTCGCCGGCGCGCGCCGCTTCCACCGCCGCGTTCAgggg > 1:5038773/1‑40 (MQ=37) tGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGt > 1:3682060/1‑70 (MQ=255) ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTaa > 1:289922/1‑71 (MQ=255) ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTaa > 1:3922155/1‑71 (MQ=255) ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTaa > 1:2614643/1‑71 (MQ=255) tCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat > 1:191333/1‑46 (MQ=255) tCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat > 1:719737/1‑46 (MQ=255) tCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat > 1:3076514/1‑46 (MQ=255) tCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat > 1:299026/1‑46 (MQ=255) tCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat > 1:2596079/1‑46 (MQ=255) tCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat > 1:5187847/1‑46 (MQ=255) tCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat > 1:512322/1‑46 (MQ=255) cGCCCGCGCGCGCCGCTTCCACGGCGGCGTTCAGGGCCAGGATATTGGTCTGGAAGGCGatgcc > 1:1993573/1‑61 (MQ=255) gCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAatat < 1:2639550/62‑1 (MQ=255) gCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTCGTCTGAAACGCAATATCGTTAATCa < 1:4862538/71‑1 (MQ=255) ccGGCGCGCGCCGCTTCCACCGCCGCGTTCAGCGCCAGGATATTGGTCTGGAAGGc < 1:3308771/56‑1 (MQ=25) ccGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAg < 1:3550580/71‑1 (MQ=255) gCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCg > 1:3789691/1‑70 (MQ=255) gCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCg > 1:5128810/1‑70 (MQ=255) cACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGaa < 1:1715739/71‑1 (MQ=255) cACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGaa < 1:1834508/71‑1 (MQ=255) cACGCGCCGCTTCCAACGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGaa < 1:4237223/71‑1 (MQ=255) gcgcCGCTTCGACCGCCGCGTTCAGCGCCAGGATATTGGTCTGGAAGGCGATGCCGTCGATCACCGagat < 1:4356027/70‑4 (MQ=255) gcgcCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTa < 1:2718333/71‑1 (MQ=255) gcgcCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTa < 1:5057359/71‑1 (MQ=255) cgccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTaa < 1:4932045/71‑1 (MQ=255) gccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTaa < 1:697639/70‑1 (MQ=255) gccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTaa > 1:4989854/1‑70 (MQ=255) gccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAAt > 1:885271/1‑71 (MQ=255) gccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAAt > 1:3446729/1‑71 (MQ=255) gccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAAt > 1:2278701/1‑71 (MQ=255) gccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAAt > 1:2466648/1‑71 (MQ=255) ccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATg < 1:1426218/71‑1 (MQ=255) ccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATg < 1:601432/71‑1 (MQ=255) ccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATg < 1:4930800/71‑1 (MQ=255) ccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATg < 1:2987709/71‑1 (MQ=255) ccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATg < 1:1844499/71‑1 (MQ=255) ccgcTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATg < 1:5015128/71‑1 (MQ=255) ccACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGcc < 1:79837/70‑1 (MQ=255) aCCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAAt > 1:3719988/1‑50 (MQ=255) aCCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATc > 1:1051065/1‑51 (MQ=255) aCCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATc > 1:15331/1‑51 (MQ=255) aCCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATc > 1:2244833/1‑51 (MQ=255) aCCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATc > 1:5083953/1‑51 (MQ=255) aCCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATc > 1:4885081/1‑51 (MQ=255) aCCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAAt > 1:2473425/1‑71 (MQ=255) aCCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAAt > 1:5133052/1‑71 (MQ=255) aCCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAAt > 1:713830/1‑71 (MQ=255) aCCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAAt > 1:4538841/1‑71 (MQ=255) gccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCg > 1:4408153/1‑42 (MQ=255) gccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAg > 1:2503798/1‑50 (MQ=255) gccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAg > 1:2867355/1‑50 (MQ=255) gccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAg > 1:5197193/1‑50 (MQ=255) gccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAg > 1:1553461/1‑50 (MQ=255) gccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAg > 1:4664456/1‑50 (MQ=255) gccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAg > 1:797935/1‑50 (MQ=255) gccgcATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAg > 1:1131523/1‑50 (MQ=255) cATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCaa > 1:1696594/1‑63 (MQ=255) cATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCaa > 1:3384665/1‑63 (MQ=255) | ACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCGAAGTAATGGTGCCAAT > minE/2068608‑2068739 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |