| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,253,075 | G→C | 31.3% | S1321S (TCC→TCG) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,253,075 | 0 | G | C | 31.3% | 88.0 / 57.7 | 67 | S1321S (TCC→TCG) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base G (29/17); new base C (13/8); total (42/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
TCGTCGCGTTTGCCCGCAACGGCTGCTTCGGTCAGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATACCCAGCAGATCGCGGGAGTAAGTTG > minE/2253010‑2253139 | tcgtcgCGTTTGCCCGCAACGGCTGCTTCGGTCAGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGAt > 1:446644/1‑71 (MQ=255) gtcgCGTTTGCCCGCAACGGCTGCTTCGGTCAGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGa < 1:4677691/71‑1 (MQ=255) gtcgCGTTTGCCCGCAACGGCTGCTTCGGTCAGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGa < 1:3792987/71‑1 (MQ=255) tggccGCAACGGCTGCTTCGGTCAGCACGCGAGTGGTGTCCTGGAACGATGCCGCGGAGATGAAGGACTc < 1:4065730/67‑1 (MQ=255) gCCCGCAACGGCTGCTTCGGTCAGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCg < 1:1987193/70‑1 (MQ=255) ccGCAACGGCTGCTTCGGTCAGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGtt > 1:1041079/1‑71 (MQ=255) ccGCAACGGCTGCTTCGGTCAGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGtt > 1:4323849/1‑71 (MQ=255) tCGGTCAGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCttt > 1:3491352/1‑71 (MQ=255) cGGTCAGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAggt > 1:3801863/1‑48 (MQ=255) cGGTCAGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCtt < 1:1634887/69‑1 (MQ=255) ggTCAGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTgg < 1:1251468/71‑1 (MQ=255) gTCAGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGt > 1:2425062/1‑71 (MQ=255) aGCACGGGGGTGGTTTCCTGGAACGATGCGGCCGAGATGaac < 1:2737307/42‑2 (MQ=255) aGCACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGaac < 1:2295718/42‑2 (MQ=255) aGCACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGaac < 1:2681639/42‑2 (MQ=255) aGCACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGaac < 1:4302292/42‑2 (MQ=255) aGCACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGaac < 1:4396902/42‑2 (MQ=255) aGCACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGaac < 1:1256645/42‑2 (MQ=255) aGCACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGaac < 1:1253965/42‑2 (MQ=255) aGCACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGaac < 1:1224086/42‑2 (MQ=255) aGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGa > 1:3678840/1‑70 (MQ=255) aGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGa > 1:2187203/1‑70 (MQ=255) aGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGa > 1:4706076/1‑70 (MQ=255) aGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAACGACTCGGTTGCCa > 1:2363949/1‑55 (MQ=255) gCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATa < 1:638836/71‑1 (MQ=255) gCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATa < 1:1362392/71‑1 (MQ=255) gCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATa < 1:3874895/71‑1 (MQ=255) gCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATa < 1:845628/71‑1 (MQ=255) gCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATa < 1:3052781/71‑1 (MQ=255) cACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGAACGa > 1:838693/1‑42 (MQ=255) cACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGAACGa > 1:3768434/1‑42 (MQ=255) cACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGAACGa > 1:860139/1‑42 (MQ=255) cACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGAACGa > 1:3801056/1‑42 (MQ=255) cACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGAACGa > 1:58809/1‑42 (MQ=255) cACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGAACGa > 1:4950113/1‑42 (MQ=255) cACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGAACGa > 1:1059048/1‑42 (MQ=255) cACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGAACGa > 1:357429/1‑42 (MQ=255) cACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGAACGa > 1:2832008/1‑42 (MQ=255) cACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGAACGa > 1:1504887/1‑42 (MQ=255) cACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGAACGa > 1:1985820/1‑42 (MQ=255) cACGCGGGTGGTTTCCTGGAACGATGCGGCCGAGATGAACGa > 1:2244842/1‑42 (MQ=255) cACGCGGGTGGTTTCCTGGAACGACGCGGCCGAGATGAACGa > 1:4894285/1‑42 (MQ=16) cACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATa < 1:2065545/70‑1 (MQ=255) cACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATAc > 1:4366853/1‑71 (MQ=255) ctcCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATACCCAGCAGATcgc > 1:1149570/1‑71 (MQ=255) ctcCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATACCCAGCAGATcgc > 1:127303/1‑71 (MQ=255) cTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCtt < 1:3973221/47‑1 (MQ=255) cTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCt > 1:2139130/1‑46 (MQ=255) cTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGt > 1:877257/1‑51 (MQ=255) cTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGt > 1:4074258/1‑51 (MQ=255) cTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGt > 1:4051475/1‑51 (MQ=255) cTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATACCCAGCAGATCGCgg < 1:1870147/70‑1 (MQ=255) cTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATACCCAGCAGATCGCgg > 1:3199377/1‑70 (MQ=255) cTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATACCCAGCAGATCGCgg < 1:4253263/70‑1 (MQ=255) gAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGAt > 1:3636370/1‑51 (MQ=255) gAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGAt > 1:2321255/1‑51 (MQ=255) gAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGAt > 1:1778829/1‑51 (MQ=255) gAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGAt > 1:3847572/1‑51 (MQ=255) gAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATACCCAGCAGATCGCGGGAg > 1:1242992/1‑70 (MQ=255) aCGCTGCCGCGGAGATGAAGGACTCGGTTGCCAgaga > 1:2061427/1‑37 (MQ=255) aCGATGCCGCGGAGATGAAGGACTCGGTTGCCaga > 1:1988304/1‑35 (MQ=255) aCGATGCCGCGGAGATGAAGGACTCGGTTGCCAgaga > 1:5085138/1‑37 (MQ=255) aCGATGCCGCGGAGATGAAGGACTCGGTTGCCAgaga > 1:705834/1‑37 (MQ=255) aCGATGCCGCGGAGATGAAGGACTCGGTTGCCAgaga > 1:5100485/1‑37 (MQ=255) aCGATGCCGCGGAGATGAAGGACTCGGTTGCCAgaga > 1:4865944/1‑37 (MQ=255) tGCCGCGGAGATGAAGGACTCGGTTGCCAGAGATGCTTTGGTGATACCCAGCAGATCGCGGGAGTAAGTTg < 1:2661028/71‑1 (MQ=255) tGCCGCGGAGATGAAGGACTCCGTTGCCAGAGACGCTTTGGTGATACCCAGCAGATCGCGGGAGTAAGTTg < 1:408744/71‑1 (MQ=255) gcgGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGt < 1:1682570/38‑1 (MQ=255) cgGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGt < 1:5145576/37‑1 (MQ=255) | TCGTCGCGTTTGCCCGCAACGGCTGCTTCGGTCAGCACGCGAGTGGTCTCCTGGAACGATGCCGCGGAGATGAAGGACTCGGTTGCCAGAGACGCTTTGGTGATACCCAGCAGATCGCGGGAGTAAGTTG > minE/2253010‑2253139 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |