| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,254,740 | A→G | 10.8% | G766G (GGT→GGC) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,254,740 | 0 | A | G | 10.8% | 256.4 / 18.0 | 95 | G766G (GGT→GGC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base A (43/41); new base G (6/4); total (50/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.81e-01 | |||||||||||
TTCAGTGCGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACG > minE/2254672‑2254808 | ttCAGTGCGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAcc > 1:115373/1‑71 (MQ=255) cAGCGCCGTATCCGCCAGACCTTTACGAGCGCCGTGGGTCGAGATGAAGTACTGCAGAACGTTCAGTCCtt > 1:750925/1‑71 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGa > 1:5107191/1‑62 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:3612339/1‑63 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:4386182/1‑63 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:4585495/1‑63 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:2289451/1‑63 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:4220552/1‑63 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:1644566/1‑63 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:5072010/1‑63 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:1410621/1‑63 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:1301247/1‑63 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:649130/1‑63 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:753149/1‑63 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:812465/1‑63 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:1070557/1‑63 (MQ=255) cGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAc > 1:2804398/1‑63 (MQ=255) tATCCGCCAGACCTTTACGAGCACCGTGGGTGGAAATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAg > 1:4768096/1‑71 (MQ=255) tATCCGCCAGACCTTTACGAGCACCGTCGGTGGAAATGAAGTACTGGAGTACGTTCAGACCTTCACGGaa > 1:3912804/1‑70 (MQ=255) tATCCGCCAGACCTTTACGAGCACCGAGGGTGGAAATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAg > 1:4922957/1‑71 (MQ=255) cGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTGCGTTCAGACCTTCACGGAAGtt > 1:4246128/1‑69 (MQ=255) cGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGaa > 1:1829973/1‑66 (MQ=255) cGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGtt > 1:3861999/1‑69 (MQ=255) cGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGtt > 1:18555/1‑69 (MQ=255) cGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTc < 1:712658/70‑1 (MQ=255) cGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTc < 1:1048281/70‑1 (MQ=255) cGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTc < 1:1835123/70‑1 (MQ=255) cGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTc < 1:17428/70‑1 (MQ=255) cGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTc < 1:1205087/70‑1 (MQ=255) gCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTc < 1:3495751/69‑1 (MQ=255) gACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCa > 1:4848455/1‑56 (MQ=255) gACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGt > 1:3964406/1‑70 (MQ=255) gACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGt > 1:1590413/1‑70 (MQ=255) cTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGt < 1:482234/60‑1 (MQ=255) cTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGt < 1:4914160/60‑1 (MQ=255) cTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGt < 1:1133119/60‑1 (MQ=255) cTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGt < 1:3699838/60‑1 (MQ=255) tACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGAca > 1:3035947/1‑45 (MQ=255) cGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGt < 1:3602964/71‑1 (MQ=255) cACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCg < 1:3680419/71‑1 (MQ=255) cACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCg < 1:3674579/71‑1 (MQ=255) cACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCg < 1:1036823/71‑1 (MQ=255) cACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCg < 1:866263/71‑1 (MQ=255) cACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCg < 1:2490041/71‑1 (MQ=255) ccGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgat < 1:3583096/71‑1 (MQ=255) gtgggtggAGATGAAGTACTGGAGTACGTTCAGACCtt > 1:4664979/1‑38 (MQ=255) gtgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatga > 1:1140902/1‑71 (MQ=255) gtgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatga > 1:2753853/1‑71 (MQ=255) gtgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatga > 1:4459948/1‑71 (MQ=255) gtgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatga < 1:2328737/71‑1 (MQ=255) gtgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatga > 1:2141417/1‑71 (MQ=255) tgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatgat > 1:4466266/1‑71 (MQ=255) tgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatgat > 1:3934677/1‑71 (MQ=255) ggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGatgatg < 1:250970/70‑1 (MQ=255) tggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGt < 1:5082925/42‑1 (MQ=255) gagaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGc > 1:1973328/1‑70 (MQ=255) agaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCa < 1:3638102/71‑1 (MQ=255) gaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTcg < 1:504150/41‑1 (MQ=255) gaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTcg < 1:3058502/41‑1 (MQ=255) gaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTcg < 1:1291735/41‑1 (MQ=255) gaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTcg < 1:4113205/41‑1 (MQ=255) gaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCAt < 1:1619030/71‑1 (MQ=255) gaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCAt < 1:3370327/71‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:740292/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:2083262/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:4158060/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:593299/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:2658214/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:4338288/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:5078207/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCgg > 1:3747987/1‑71 (MQ=255) aGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGc < 1:3297121/71‑1 (MQ=255) aGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGc < 1:2367583/71‑1 (MQ=255) ggAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCg > 1:3662170/1‑69 (MQ=255) ggAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGc > 1:3499390/1‑70 (MQ=255) ggAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGc > 1:2802122/1‑70 (MQ=255) ggAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGc > 1:4010258/1‑70 (MQ=255) tACGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCgg < 1:3306006/60‑1 (MQ=255) tACGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCgg < 1:4690708/60‑1 (MQ=255) tACGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCgg < 1:3641668/60‑1 (MQ=255) aCGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCgg < 1:1160143/59‑1 (MQ=255) cGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCGGCt > 1:4047195/1‑60 (MQ=255) cGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCGGCt > 1:1581500/1‑60 (MQ=255) cGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCGGCt > 1:1737997/1‑60 (MQ=255) cGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCGGCt > 1:294702/1‑60 (MQ=255) cGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCGGCt > 1:2760828/1‑60 (MQ=255) agTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCGGCt > 1:461485/2‑60 (MQ=255) ttCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGAcc < 1:1159017/71‑1 (MQ=255) ttCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGAc > 1:4213329/1‑70 (MQ=255) ttCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGAc > 1:1283729/1‑70 (MQ=255) cAGAGCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCAc > 1:1040516/1‑71 (MQ=255) cAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCAc > 1:3658936/1‑71 (MQ=255) aGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCAc < 1:3303827/70‑1 (MQ=255) aGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCAc < 1:4016088/70‑1 (MQ=255) aGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACg < 1:3205649/71‑1 (MQ=255) aGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATACGGCTTCGCCATCAGACCAc < 1:3090693/70‑1 (MQ=255) | TTCAGTGCGGTATCCGCCAGACCTTTACGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACG > minE/2254672‑2254808 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |