| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,254,767 | C→G | 13.5% | T757T (ACG→ACC) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,254,767 | 0 | C | G | 13.5% | 194.0 / 19.8 | 74 | T757T (ACG→ACC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base C (23/41); new base G (6/4); total (29/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAATCTGTGC > minE/2254699‑2254835 | cGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGt < 1:3602964/71‑1 (MQ=255) cACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCg < 1:3674579/71‑1 (MQ=255) cACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCg < 1:3680419/71‑1 (MQ=255) cACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCg < 1:2490041/71‑1 (MQ=255) cACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCg < 1:1036823/71‑1 (MQ=255) cACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCg < 1:866263/71‑1 (MQ=255) ccGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgat < 1:3583096/71‑1 (MQ=255) gtgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatga > 1:1140902/1‑71 (MQ=255) gtgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatga > 1:2141417/1‑71 (MQ=255) gtgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatga < 1:2328737/71‑1 (MQ=255) gtgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatga > 1:4459948/1‑71 (MQ=255) gtgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatga > 1:2753853/1‑71 (MQ=255) tgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatgat > 1:4466266/1‑71 (MQ=255) tgggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCgatgat > 1:3934677/1‑71 (MQ=255) ggtggAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGatgatg < 1:250970/70‑1 (MQ=255) gagaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGc > 1:1973328/1‑70 (MQ=255) agaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCa < 1:3638102/71‑1 (MQ=255) gaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCAt < 1:3370327/71‑1 (MQ=255) gaTGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCAt < 1:1619030/71‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:740292/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:4158060/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:2658214/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:4338288/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:2083262/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:5078207/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGttt < 1:593299/52‑1 (MQ=255) aaGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCgg > 1:3747987/1‑71 (MQ=255) aGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGc < 1:2367583/71‑1 (MQ=255) aGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGc < 1:3297121/71‑1 (MQ=255) ggAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCg > 1:3662170/1‑69 (MQ=255) ggAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGc > 1:2802122/1‑70 (MQ=255) ggAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGc > 1:4010258/1‑70 (MQ=255) ggAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGc > 1:3499390/1‑70 (MQ=255) tACGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCgg < 1:4690708/60‑1 (MQ=255) tACGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCgg < 1:3641668/60‑1 (MQ=255) tACGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCgg < 1:3306006/60‑1 (MQ=255) aCGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCgg < 1:1160143/59‑1 (MQ=255) cGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCGGCt > 1:4047195/1‑60 (MQ=255) cGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCGGCt > 1:294702/1‑60 (MQ=255) cGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCGGCt > 1:2760828/1‑60 (MQ=255) cGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCGGCt > 1:1737997/1‑60 (MQ=255) cGTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCGGCt > 1:1581500/1‑60 (MQ=255) agTTCAGGCCTTCCCGGAAGTTCGCGGTGATCGGGGTCTCGATGATCGAGCCGTCCGGCt > 1:461485/2‑60 (MQ=255) ttCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGAcc < 1:1159017/71‑1 (MQ=255) ttCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGAc > 1:1283729/1‑70 (MQ=255) ttCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGAc > 1:4213329/1‑70 (MQ=255) cAGAGCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCAc > 1:1040516/1‑71 (MQ=255) cAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCAc > 1:3658936/1‑71 (MQ=255) aGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCAc < 1:3303827/70‑1 (MQ=255) aGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCAc < 1:4016088/70‑1 (MQ=255) aGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACg < 1:3205649/71‑1 (MQ=255) aGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATACGGCTTCGCCATCAGACCAc < 1:3090693/70‑1 (MQ=255) cTTCACGGAAGTTCGCGGTGATTGGCGTTTCGAAGATGGAGCCATCCgg < 1:2967508/49‑1 (MQ=255) ttCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCgg < 1:4973791/48‑1 (MQ=255) tCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCa < 1:886499/67‑1 (MQ=255) cACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCa < 1:4818177/66‑1 (MQ=255) cACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATAc > 1:3823471/1‑69 (MQ=255) cACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATAc > 1:1726566/1‑69 (MQ=255) cGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGc < 1:2769271/71‑1 (MQ=255) cGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGc < 1:1202514/71‑1 (MQ=255) cGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGc < 1:7006/71‑1 (MQ=255) cGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGc < 1:4048458/71‑1 (MQ=255) cGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGc < 1:4027435/71‑1 (MQ=255) ggAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGc > 1:1557053/1‑70 (MQ=255) aGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCgg < 1:2906056/40‑1 (MQ=255) aGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGc > 1:3509438/1‑71 (MQ=255) cgGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGa < 1:1402842/71‑1 (MQ=255) gTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAAt < 1:4762503/71‑1 (MQ=255) gTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAAt > 1:3248259/1‑71 (MQ=255) gTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAAt > 1:4995036/1‑71 (MQ=255) gTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAAt < 1:5072192/71‑1 (MQ=255) gTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAAt < 1:4459727/71‑1 (MQ=255) tGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAATc > 1:1870126/1‑71 (MQ=255) gCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAATCTGTGc < 1:5027828/70‑1 (MQ=255) | CGAGCACCGTGGGTGGAGATGAAGTACTGGAGTACGTTCAGACCTTCACGGAAGTTCGCGGTGATTGGCGTTTCGATGATGGAGCCATCCGGCTTCGCCATCAGACCACGCATACCAGCAAGCTGACGAATCTGTGC > minE/2254699‑2254835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |