| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,256,930 | A→G | 40.0% | G36G (GGT→GGC) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,256,930 | 0 | A | G | 40.0% | 14.1 / 30.2 | 30 | G36G (GGT→GGC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base A (9/9); new base G (10/2); total (19/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01 | |||||||||||
CGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGCAATTTTGATCGCATCAAACTCTTCGGTTT > minE/2256863‑2257000 | cgcAGAACAGGCCGTCACGCTCGGGCTTGAACGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCg < 1:2182914/71‑1 (MQ=255) gcAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGa < 1:2831007/71‑1 (MQ=255) aCGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGa > 1:5030987/1‑71 (MQ=255) aCGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGa > 1:3101332/1‑71 (MQ=255) cGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGAt < 1:4515245/71‑1 (MQ=255) cGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGAt < 1:3424670/71‑1 (MQ=255) tGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGa > 1:2177154/1‑54 (MQ=255) tGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGa > 1:4367366/1‑54 (MQ=255) tGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGa > 1:3485529/1‑54 (MQ=255) ttGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTgg > 1:2651020/1‑71 (MQ=255) tGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGc > 1:3553161/1‑71 (MQ=255) aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGCCTTCGCCGAACGACCac > 1:479513/1‑49 (MQ=255) aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCac > 1:3545429/1‑49 (MQ=255) aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCac > 1:2659087/1‑49 (MQ=255) aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCac > 1:1149082/1‑49 (MQ=255) aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCac > 1:3932613/1‑49 (MQ=255) aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCac > 1:4076191/1‑49 (MQ=255) aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCac > 1:2425425/1‑49 (MQ=255) aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCa > 1:1579324/1‑49 (MQ=255) gTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGCGAAGc < 1:5200264/71‑1 (MQ=255) gTAGTTGATGGTTTCCGTCTTTTTAACTTCACCGAAAGACCATGAACGGAt < 1:548856/51‑1 (MQ=255) tAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGc < 1:688668/71‑1 (MQ=255) tAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGc < 1:3557894/71‑1 (MQ=255) tAGTTGATGGGTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGc < 1:498367/71‑1 (MQ=255) aGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAGCGACCACGAACGGATTTTTTCAGGCGACGCCaggcc > 1:913239/1‑67 (MQ=255) aGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCACGAACGGATTTTTTCAGGCGACGCCaggcc > 1:882898/1‑67 (MQ=255) ttGATGGTTTCCGGCTTTTTAACTTCGCCGAAAGACCagg < 1:4095707/40‑3 (MQ=38) ggTTTCCGGCTTTTTAACTTCACAGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGCAATTTTg < 1:1946962/70‑1 (MQ=255) tCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTc > 1:4703722/1‑44 (MQ=255) tCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTc > 1:87794/1‑44 (MQ=255) aCCGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGCAATTTTGATCGCATCAAACTCTTCGGttt < 1:2813603/71‑1 (MQ=255) | CGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGCAATTTTGATCGCATCAAACTCTTCGGTTT > minE/2256863‑2257000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |