Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,256,930 A→G 40.0% G36G (GGT→GGC rpoC ← RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,256,9300AG40.0% 14.1 / 30.2 30G36G (GGT→GGCrpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (9/9);  new base G (10/2);  total (19/11)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

CGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGCAATTTTGATCGCATCAAACTCTTCGGTTT  >  minE/2256863‑2257000
                                                                   |                                                                      
cgcAGAACAGGCCGTCACGCTCGGGCTTGAACGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCg                                                                     <  1:2182914/71‑1 (MQ=255)
 gcAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGa                                                                    <  1:2831007/71‑1 (MQ=255)
                aCGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGa                                                     >  1:5030987/1‑71 (MQ=255)
                aCGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGa                                                     >  1:3101332/1‑71 (MQ=255)
                 cGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGAt                                                    <  1:4515245/71‑1 (MQ=255)
                 cGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGAt                                                    <  1:3424670/71‑1 (MQ=255)
                      tGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGa                                                                >  1:2177154/1‑54 (MQ=255)
                      tGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGa                                                                >  1:4367366/1‑54 (MQ=255)
                      tGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGa                                                                >  1:3485529/1‑54 (MQ=255)
                          ttGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTgg                                           >  1:2651020/1‑71 (MQ=255)
                           tGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGc                                          >  1:3553161/1‑71 (MQ=255)
                              aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGCCTTCGCCGAACGACCac                                                            >  1:479513/1‑49 (MQ=255)
                              aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCac                                                            >  1:3545429/1‑49 (MQ=255)
                              aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCac                                                            >  1:2659087/1‑49 (MQ=255)
                              aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCac                                                            >  1:1149082/1‑49 (MQ=255)
                              aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCac                                                            >  1:3932613/1‑49 (MQ=255)
                              aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCac                                                            >  1:4076191/1‑49 (MQ=255)
                              aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCac                                                            >  1:2425425/1‑49 (MQ=255)
                              aCGTACGGTAGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCa                                                             >  1:1579324/1‑49 (MQ=255)
                                gTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGCGAAGc                                     <  1:5200264/71‑1 (MQ=255)
                                     gTAGTTGATGGTTTCCGTCTTTTTAACTTCACCGAAAGACCATGAACGGAt                                                    <  1:548856/51‑1 (MQ=255)
                                      tAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGc                               <  1:688668/71‑1 (MQ=255)
                                      tAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGc                               <  1:3557894/71‑1 (MQ=255)
                                      tAGTTGATGGGTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGc                               <  1:498367/71‑1 (MQ=255)
                                       aGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAGCGACCACGAACGGATTTTTTCAGGCGACGCCaggcc                               >  1:913239/1‑67 (MQ=255)
                                       aGTTGATGGTTTCCGGCTTCTTGACTTCGCCGAACGACCACGAACGGATTTTTTCAGGCGACGCCaggcc                               >  1:882898/1‑67 (MQ=255)
                                         ttGATGGTTTCCGGCTTTTTAACTTCGCCGAAAGACCagg                                                           <  1:4095707/40‑3 (MQ=38)
                                              ggTTTCCGGCTTTTTAACTTCACAGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGCAATTTTg                        <  1:1946962/70‑1 (MQ=255)
                                                  tCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTc                                              >  1:4703722/1‑44 (MQ=255)
                                                  tCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTc                                              >  1:87794/1‑44 (MQ=255)
                                                                   aCCGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGCAATTTTGATCGCATCAAACTCTTCGGttt  <  1:2813603/71‑1 (MQ=255)
                                                                   |                                                                      
CGCAGAAAAGGCCGTCACGTTCTGGTTTGAACGTACGGTAGTTGATGGTTTCCGGCTTTTTAACTTCACCGAAAGACCATGAACGGATCATGTCTGGCGAAGCCAGAGCAATTTTGATCGCATCAAACTCTTCGGTTT  >  minE/2256863‑2257000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: