| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,266,165 | G→A | 16.4% | I93I (ATC→ATT) | tufB ← | protein chain elongation factor EF‑Tu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,266,165 | 0 | G | A | 16.4% | 174.9 / 21.9 | 67 | I93I (ATC→ATT) | tufB | protein chain elongation factor EF‑Tu |
| Reads supporting (aligned to +/- strand): ref base G (23/33); new base A (5/6); total (28/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGG > minE/2266109‑2266222 | gggCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTaa < 1:2551182/70‑1 (MQ=35)gggCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTaa < 1:1389953/70‑1 (MQ=35)gggCCGTCAGTCGCAGCAACTAACAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTaa < 1:1137745/70‑1 (MQ=255) gCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACa > 1:1250902/1‑70 (MQ=35) cGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAg > 1:1671253/1‑71 (MQ=34) aGGATCGCGCCGTCCATCTGCGCAGCACCGGTAATCATGttct > 1:1863934/1‑41 (MQ=14) aGGATCGCGCCGTCCATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGt > 1:1879085/1‑70 (MQ=11) aGGATCGCGCCGTCCATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTc < 1:297206/71‑1 (MQ=11) aGGATCGCGCCGTCCATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTc < 1:268631/71‑1 (MQ=11) aGGATCGCGCCGTCCATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTc < 1:4985370/71‑1 (MQ=11) aGGATCGCGCCGTCCATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTc < 1:712045/71‑1 (MQ=11) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAg < 1:4657540/49‑1 (MQ=38) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAg < 1:2950939/49‑1 (MQ=38) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAg < 1:3536029/49‑1 (MQ=38) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAg < 1:178601/49‑1 (MQ=38) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCggg < 1:801683/64‑1 (MQ=35) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCggg < 1:2677146/64‑1 (MQ=35) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCggg < 1:3995979/64‑1 (MQ=35) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCggg < 1:2438177/64‑1 (MQ=35) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc > 1:2926817/1‑49 (MQ=38) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc > 1:2817966/1‑49 (MQ=38) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc > 1:3045157/1‑49 (MQ=38) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc > 1:3052658/1‑49 (MQ=38) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc > 1:3848834/1‑49 (MQ=38) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc > 1:535194/1‑49 (MQ=38) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc > 1:1080182/1‑49 (MQ=38) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc > 1:4300656/1‑49 (MQ=38) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGcc > 1:3194415/1‑57 (MQ=35) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc > 1:1514593/1‑70 (MQ=35) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACg > 1:3369030/1‑71 (MQ=34) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACg > 1:4584516/1‑71 (MQ=34) tCGCGCCGTCCATCTGCGCAGCACAGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACg > 1:2615751/1‑71 (MQ=21) ccGTCCATCTGCGCAGCACCGGTGATCATGTTTTCAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGc > 1:2021762/1‑71 (MQ=21) cggccATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:1234672/68‑1 (MQ=21) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:1024165/71‑1 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:3536838/71‑1 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:5083395/71‑1 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg < 1:2576480/71‑1 (MQ=34) gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTTCCCCGGGCAGTCTACGTGTGCGt < 1:116911/71‑1 (MQ=21) gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt < 1:1005779/71‑1 (MQ=34) gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt < 1:758690/71‑1 (MQ=34) tCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTa < 1:4593377/71‑1 (MQ=34) ccATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTCAACGTGCGCGTa > 1:2374542/1‑70 (MQ=2) ccATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTCAACGTGCGCGTa > 1:2323996/1‑70 (MQ=2) ccATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTCAACGTGCGCGTAg > 1:4104371/1‑71 (MQ=2) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCg > 1:3382242/1‑43 (MQ=38) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCg > 1:5064879/1‑43 (MQ=38) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCg > 1:570476/1‑43 (MQ=38) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAg < 1:2274659/70‑1 (MQ=35) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAg < 1:1112507/70‑1 (MQ=35) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt > 1:4327106/1‑71 (MQ=34) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt > 1:2603834/1‑71 (MQ=34) cATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTCAACGTGAGCGTAGt < 1:4400135/71‑1 (MQ=2) cATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTCAACGTGAGCGTAGt < 1:1730082/71‑1 (MQ=2) tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:88499/69‑1 (MQ=35) tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:1075632/69‑1 (MQ=35) tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:770768/69‑1 (MQ=35) tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:1465794/69‑1 (MQ=35) tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:4568808/69‑1 (MQ=35) tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:2374937/69‑1 (MQ=35) tCTGCGCAGCACCGGTGATCATGTGTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:738858/69‑1 (MQ=25) cTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt < 1:3748934/68‑1 (MQ=35) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTGTACGTGTGCGTAGTGACg > 1:2382090/1‑71 (MQ=21) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACg > 1:2796724/1‑71 (MQ=34) cgcAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACgg < 1:1102974/70‑1 (MQ=31) cgcAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACgg < 1:1077606/70‑1 (MQ=31) cgcAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACgg < 1:3849002/70‑1 (MQ=31) | GGGCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGG > minE/2266109‑2266222 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |