| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,396,396 | C→G | 11.9% | V264V (GTG→GTC) | corA ← | magnesium/nickel/cobalt transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,396,396 | 0 | C | G | 11.9% | 179.5 / 13.9 | 67 | V264V (GTG→GTC) | corA | magnesium/nickel/cobalt transporter |
| Reads supporting (aligned to +/- strand): ref base C (37/22); new base G (3/5); total (40/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
TTCAGTTCTGGCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGC > minE/2396328‑2396465 | ttCAGTTCTGGCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCAc > 1:2626403/1‑71 (MQ=255)ttCAGTTCTGGCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCAc > 1:5075900/1‑71 (MQ=255)ttCAGTTCTGGCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCAc > 1:4681309/1‑71 (MQ=255)ttCAGTTCTGGCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCAc > 1:1489538/1‑71 (MQ=255) gCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATacca < 1:4314796/69‑1 (MQ=255) aaCTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCgag > 1:3349645/1‑69 (MQ=255) aaCTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCgag > 1:4509401/1‑69 (MQ=255) aaCTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCgag > 1:4151875/1‑69 (MQ=255) tCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGAt < 1:4545789/57‑1 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATag > 1:1389904/1‑54 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac > 1:4255323/1‑55 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac > 1:2692619/1‑55 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac > 1:1920749/1‑55 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac > 1:175046/1‑55 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac > 1:163453/1‑55 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac > 1:4434747/1‑55 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac > 1:477543/1‑55 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt > 1:3837953/1‑70 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt > 1:2085929/1‑70 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt > 1:3351686/1‑70 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt > 1:3221235/1‑70 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt > 1:2932107/1‑70 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt > 1:5139216/1‑70 (MQ=255) aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACCGATac > 1:4440803/1‑55 (MQ=255) ttCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat < 1:401522/71‑1 (MQ=255) ttCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat < 1:1142886/71‑1 (MQ=255) tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACTGAGAAGATTTTgatgat > 1:3294223/1‑70 (MQ=255) tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTgatgat > 1:98226/1‑70 (MQ=255) tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGatgatg > 1:2458664/1‑71 (MQ=255) gCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCt > 1:2531082/1‑71 (MQ=255) gCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCt > 1:3331190/1‑71 (MQ=255) gAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTc < 1:2526692/71‑1 (MQ=255) gAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGAAGATGCGGTTCTGCTc < 1:4773092/71‑1 (MQ=255) aaGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat < 1:1574528/55‑1 (MQ=255) aGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGa < 1:2167132/71‑1 (MQ=255) aGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGCTCTGCTCGa < 1:3130823/71‑1 (MQ=255) gCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGAt < 1:3354515/71‑1 (MQ=255) gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa < 1:1813713/71‑1 (MQ=255) gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa < 1:2447158/71‑1 (MQ=255) gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa < 1:5057904/71‑1 (MQ=255) gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa < 1:5072763/71‑1 (MQ=255) gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa < 1:549962/71‑1 (MQ=255) tCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATaaaa > 1:258502/1‑71 (MQ=255) ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaagc > 1:4217374/1‑68 (MQ=255) ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATGAAGcc > 1:1692191/1‑71 (MQ=255) ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATGAAGcc > 1:4184570/1‑71 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt < 1:1228375/61‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt < 1:1047516/61‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt < 1:3384193/61‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt < 1:4141456/61‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt < 1:3557998/61‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt < 1:1777772/61‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAtt < 1:1087850/71‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAtt < 1:2477986/71‑1 (MQ=255) cAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:812618/1‑69 (MQ=255) cAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:3800745/1‑69 (MQ=255) aGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATaa > 1:251984/1‑61 (MQ=255) gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:1695809/1‑66 (MQ=255) gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:2162886/1‑66 (MQ=255) gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:1675692/1‑66 (MQ=255) ataccacggataccacggAGAAGATTTTGATGCTGCGGTTCTGCTCGATGTTGATAAAAccc > 1:2891766/1‑62 (MQ=255) aTACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATa > 1:4469958/1‑56 (MQ=255) aTACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAAccc > 1:4330856/1‑62 (MQ=255) tACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCt > 1:1174252/1‑71 (MQ=255) aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc < 1:3525462/42‑1 (MQ=255) aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc < 1:1383812/42‑1 (MQ=255) aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc < 1:699964/42‑1 (MQ=255) aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc < 1:1085906/42‑1 (MQ=255) ccACGGATACCACCGAGAAGATTTTTATGATGCTGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc < 1:2257654/71‑1 (MQ=255) ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCTATGTTGATAAAACCCATTGCCGCCTGc < 1:4529187/71‑1 (MQ=255) ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc < 1:4311684/71‑1 (MQ=255) ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc < 1:824486/71‑1 (MQ=255) cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg < 1:2826970/46‑1 (MQ=255) cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg < 1:1131899/46‑1 (MQ=255) cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg < 1:1138150/46‑1 (MQ=255) | TTCAGTTCTGGCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGC > minE/2396328‑2396465 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |