| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,396,417 | T→C | 9.5% | K257K (AAA→AAG) | corA ← | magnesium/nickel/cobalt transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,396,417 | 0 | T | C | 9.5% | 238.7 / 12.8 | 84 | K257K (AAA→AAG) | corA | magnesium/nickel/cobalt transporter |
| Reads supporting (aligned to +/- strand): ref base T (30/46); new base C (3/5); total (33/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
AGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTGG > minE/2396350‑2396486 | aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt > 1:5139216/1‑70 (MQ=255)aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt > 1:3837953/1‑70 (MQ=255)aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt > 1:3351686/1‑70 (MQ=255)aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt > 1:3221235/1‑70 (MQ=255)aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt > 1:2932107/1‑70 (MQ=255)aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt > 1:2085929/1‑70 (MQ=255) ttCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat < 1:401522/71‑1 (MQ=255) ttCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat < 1:1142886/71‑1 (MQ=255) tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACTGAGAAGATTTTgatgat > 1:3294223/1‑70 (MQ=255) tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTgatgat > 1:98226/1‑70 (MQ=255) tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGatgatg > 1:2458664/1‑71 (MQ=255) gCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCt > 1:2531082/1‑71 (MQ=255) gCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCt > 1:3331190/1‑71 (MQ=255) gAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTc < 1:2526692/71‑1 (MQ=255) gAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGAAGATGCGGTTCTGCTc < 1:4773092/71‑1 (MQ=255) aaGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat < 1:1574528/55‑1 (MQ=255) aGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGa < 1:2167132/71‑1 (MQ=255) aGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGCTCTGCTCGa < 1:3130823/71‑1 (MQ=255) gCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGAt < 1:3354515/71‑1 (MQ=255) gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa < 1:549962/71‑1 (MQ=255) gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa < 1:2447158/71‑1 (MQ=255) gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa < 1:1813713/71‑1 (MQ=255) gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa < 1:5057904/71‑1 (MQ=255) gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa < 1:5072763/71‑1 (MQ=255) tCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATaaaa > 1:258502/1‑71 (MQ=255) ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaagc > 1:4217374/1‑68 (MQ=255) ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATGAAGcc > 1:1692191/1‑71 (MQ=255) ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATGAAGcc > 1:4184570/1‑71 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt < 1:3384193/61‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt < 1:3557998/61‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt < 1:4141456/61‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt < 1:1228375/61‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt < 1:1047516/61‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt < 1:1777772/61‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAtt < 1:2477986/71‑1 (MQ=255) gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAtt < 1:1087850/71‑1 (MQ=255) cAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:3800745/1‑69 (MQ=255) cAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:812618/1‑69 (MQ=255) aGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATaa > 1:251984/1‑61 (MQ=255) gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:2162886/1‑66 (MQ=255) gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:1675692/1‑66 (MQ=255) gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:1695809/1‑66 (MQ=255) ataccacggataccacggAGAAGATTTTGATGCTGCGGTTCTGCTCGATGTTGATAAAAccc > 1:2891766/1‑62 (MQ=255) aTACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATa > 1:4469958/1‑56 (MQ=255) aTACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAAccc > 1:4330856/1‑62 (MQ=255) tACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCt > 1:1174252/1‑71 (MQ=255) aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc < 1:3525462/42‑1 (MQ=255) aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc < 1:699964/42‑1 (MQ=255) aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc < 1:1383812/42‑1 (MQ=255) aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc < 1:1085906/42‑1 (MQ=255) ccACGGATACCACCGAGAAGATTTTTATGATGCTGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc < 1:2257654/71‑1 (MQ=255) ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCTATGTTGATAAAACCCATTGCCGCCTGc < 1:4529187/71‑1 (MQ=255) ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc < 1:4311684/71‑1 (MQ=255) ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc < 1:824486/71‑1 (MQ=255) cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg < 1:1138150/46‑1 (MQ=255) cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg < 1:2826970/46‑1 (MQ=255) cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg < 1:1131899/46‑1 (MQ=255) aCGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTc < 1:2991302/41‑1 (MQ=255) aCGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCAt > 1:4366902/1‑71 (MQ=255) ggATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCAt > 1:2249070/1‑69 (MQ=255) ggATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCAt > 1:3429463/1‑69 (MQ=255) aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATaaaa > 1:773147/1‑50 (MQ=255) aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:3814045/1‑55 (MQ=255) aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:2268030/1‑55 (MQ=255) aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:1645515/1‑55 (MQ=255) aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:1741983/1‑55 (MQ=255) aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:4984013/1‑55 (MQ=255) aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt > 1:3312330/1‑55 (MQ=255) aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAgg < 1:2766635/71‑1 (MQ=255) aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAgg < 1:642244/71‑1 (MQ=255) aTACCACCGAGAAGATTTAGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAgg < 1:698915/71‑1 (MQ=255) tACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGa < 1:4877332/71‑1 (MQ=255) tACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGa < 1:4751950/71‑1 (MQ=255) ccaccGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAg < 1:3926750/71‑1 (MQ=255) ccaccGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAg < 1:1530667/71‑1 (MQ=255) ccaccGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAT‑AAACCCATTGCCGCCTGCATCAGGAAg < 1:3034435/70‑1 (MQ=255) gagaAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGa < 1:1948/64‑1 (MQ=255) gaagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAg > 1:3563058/1‑64 (MQ=255) gaagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAg > 1:5023836/1‑64 (MQ=255) gaagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCa < 1:822/68‑1 (MQ=255) gaagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCa < 1:1360881/68‑1 (MQ=255) aagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCtt < 1:577200/71‑1 (MQ=255) aagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCtt < 1:1391931/71‑1 (MQ=255) aagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCtt < 1:742254/71‑1 (MQ=255) aagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCtt < 1:3319705/71‑1 (MQ=255) aagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCtt < 1:1644396/71‑1 (MQ=255) agaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCtt < 1:4397177/70‑1 (MQ=255) gaTTTTGATGATGCGGTTCTGCTCGATGTTGATaaaa > 1:2043189/1‑37 (MQ=255) gaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGAATCAGGAAGTTCACCTTCt > 1:4182288/1‑71 (MQ=255) aTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTg < 1:3919714/71‑1 (MQ=255) ttttGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTgg < 1:1596639/71‑1 (MQ=255) ttttGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTgg < 1:1821522/71‑1 (MQ=255) ttttGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTgg < 1:2935005/71‑1 (MQ=255) ttttGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTgg < 1:169153/71‑1 (MQ=255) ttttGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTg < 1:2144787/70‑1 (MQ=255) ttttGATGATGCGGTGCTGCGCGATGGTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTgg < 1:3284914/71‑1 (MQ=255) tttGATGATGCGGTTCTGCTCGATGTTGATAAAAccc > 1:654501/1‑37 (MQ=255) | AGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTGG > minE/2396350‑2396486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |