| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 123,550 | A→G | 12.6% | intergenic (+177/+25) | cueO → / ← gcd | multicopper oxidase (laccase)/glucose dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 123,550 | 0 | A | G | 12.6% | 269.4 / 25.8 | 103 | intergenic (+177/+25) | cueO/gcd | multicopper oxidase (laccase)/glucose dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (48/42); new base G (9/4); total (57/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
ATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGCCACAATATAG > minE/123480‑123614 | aTCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCa > 1:2988647/1‑71 (MQ=255) tGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGt > 1:1171462/1‑71 (MQ=255) tGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGt > 1:4209872/1‑71 (MQ=255) tGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGt > 1:1287221/1‑71 (MQ=255) tGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGt > 1:535671/1‑71 (MQ=255) ttGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGc > 1:1495503/1‑70 (MQ=255) ttGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGc > 1:279495/1‑70 (MQ=255) ttGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGc > 1:2512564/1‑70 (MQ=255) aTTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAg < 1:2960430/71‑1 (MQ=255) ttttGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAg < 1:3380507/70‑1 (MQ=255) ttttGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGc < 1:4778690/71‑1 (MQ=255) ttttGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGc < 1:4246953/71‑1 (MQ=255) ggATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCt < 1:1582692/56‑1 (MQ=255) ggATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCt < 1:4003144/56‑1 (MQ=255) ggATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCt < 1:3419434/56‑1 (MQ=255) aaGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTcaca > 1:4399778/1‑71 (MQ=255) aaGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTcaca > 1:5044388/1‑71 (MQ=255) aaGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTcaca > 1:3201378/1‑71 (MQ=255) aaGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTcaca > 1:2159653/1‑71 (MQ=255) aaGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTcaca > 1:1871547/1‑71 (MQ=255) aaGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGAAAGTCTTACTTcaca > 1:4471958/1‑71 (MQ=255) ggCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCAcat > 1:3863334/1‑70 (MQ=255) ggCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCAcat > 1:2462121/1‑70 (MQ=255) gCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCAcatca < 1:2066781/71‑1 (MQ=255) ttCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTca < 1:1671422/63‑1 (MQ=255) ttCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTca < 1:3379220/63‑1 (MQ=255) ttCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTca < 1:2995317/63‑1 (MQ=255) ttCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTca < 1:4823033/63‑1 (MQ=255) ttCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTca < 1:2615997/63‑1 (MQ=255) aCGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGc < 1:4112905/71‑1 (MQ=255) tCCGGCATGAACAACTCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCAcatca < 1:492172/57‑1 (MQ=255) tCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCAcatca < 1:2445230/57‑1 (MQ=255) tCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCAcatca < 1:2618008/57‑1 (MQ=255) tCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCAcatca < 1:1245504/57‑1 (MQ=255) tCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCAcatca < 1:3083657/57‑1 (MQ=255) tCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCAcatca < 1:124561/57‑1 (MQ=255) ccGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGc < 1:1362091/62‑1 (MQ=255) ccGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGc < 1:180119/62‑1 (MQ=255) ccGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGc < 1:2661195/62‑1 (MQ=255) ccGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGc < 1:1959027/62‑1 (MQ=255) cGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACatcatc > 1:2228695/1‑57 (MQ=255) cGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACatcatc > 1:2059409/1‑57 (MQ=255) cGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACatcatc > 1:1080901/1‑57 (MQ=255) cGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACatcatc > 1:4274750/1‑57 (MQ=255) cGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGc < 1:3257209/61‑1 (MQ=255) ggCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACtt < 1:1834637/47‑1 (MQ=255) ggCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACtt < 1:2885687/47‑1 (MQ=255) ggCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAg > 1:3908905/1‑62 (MQ=255) ggCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAg > 1:2126313/1‑62 (MQ=255) ggCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAg > 1:4033520/1‑62 (MQ=255) ggCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAg > 1:3121950/1‑62 (MQ=255) ggCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAg > 1:3308964/1‑62 (MQ=255) ggCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAg > 1:4152990/1‑62 (MQ=255) ggCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAg > 1:3444632/1‑62 (MQ=255) ggCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAg > 1:3515051/1‑62 (MQ=255) ataaacaacGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGCca > 1:2222117/4‑70 (MQ=255) aTGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGCca > 1:5037588/1‑70 (MQ=255) aTGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGCca > 1:1705526/1‑70 (MQ=255) tGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCt < 1:2383851/38‑1 (MQ=255) tGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCt < 1:1799818/38‑1 (MQ=255) tGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCt < 1:4722535/38‑1 (MQ=255) tGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGCcaca < 1:4251523/71‑1 (MQ=255) tGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGCcaca < 1:2796833/71‑1 (MQ=255) tGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGCcac > 1:4176877/1‑70 (MQ=255) tGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGCcac > 1:4145266/1‑70 (MQ=255) tgacaacGTGCGCGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAgc < 1:5194924/56‑1 (MQ=38) tgacaacGTGCGCGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAgc < 1:805606/56‑1 (MQ=38) tgacaacGTGCGCGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAgc < 1:2698849/56‑1 (MQ=38) tgacaacGTGCGCGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAgc < 1:2108003/56‑1 (MQ=38) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTc > 1:1728994/1‑35 (MQ=255) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTc > 1:4790261/1‑35 (MQ=255) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTc > 1:2636692/1‑35 (MQ=255) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTc > 1:1633322/1‑35 (MQ=255) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTc > 1:2218350/1‑35 (MQ=255) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTc > 1:4666991/1‑35 (MQ=255) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTc > 1:4969919/1‑35 (MQ=255) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTc > 1:5021884/1‑35 (MQ=255) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTc > 1:5031380/1‑35 (MQ=255) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTc > 1:752411/1‑35 (MQ=255) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTc > 1:418076/1‑35 (MQ=255) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTc > 1:3663052/1‑35 (MQ=255) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGCCACaa < 1:4267466/70‑1 (MQ=255) aacaacGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGCCACaa < 1:1919328/70‑1 (MQ=255) acaacGTGCGCGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAgcgc > 1:4748103/1‑58 (MQ=255) acaacGTGCGCGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAgcgc > 1:1194893/1‑58 (MQ=255) acaacGTGCGCGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAgcgc > 1:750273/1‑58 (MQ=255) acaacGTGCGCGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAgcgc > 1:2619464/1‑58 (MQ=255) acaacGTGCGCGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAgcgc > 1:4718154/1‑58 (MQ=255) acaacGTGCGCGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAgcgc > 1:1794515/1‑58 (MQ=255) acaacGTGCGCGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAgcgc > 1:3461024/1‑58 (MQ=255) acaacGTGCGCGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAgcgc > 1:1514934/1‑58 (MQ=255) acaacGTGCGCGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAgcgc > 1:4183298/1‑58 (MQ=255) acaacGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGCCACAat < 1:605641/70‑1 (MQ=255) acGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCa > 1:2188052/1‑56 (MQ=255) acGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCa > 1:1240637/1‑56 (MQ=255) acGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGc < 1:2961292/61‑1 (MQ=255) acGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGc < 1:4381169/61‑1 (MQ=255) acGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGc < 1:3486199/61‑1 (MQ=255) acGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGc < 1:566306/61‑1 (MQ=255) acGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGCCACAATATAg > 1:3744951/1‑71 (MQ=255) | ATCCATTGATTTTGCACAATTTTGTAGGCTGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGCGCACGTTGTCAGCAATCTGAGCAAGTCTTACTTCACATCATCCGGCAGCGCATAAGCCACAATATAG > minE/123480‑123614 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |