| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,494,739 | G→A | 5.6% | P74P (CCG→CCA) | ibpB → | heat shock chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,494,739 | 0 | G | A | 5.6% | 283.2 / 4.5 | 89 | P74P (CCG→CCA) | ibpB | heat shock chaperone |
| Reads supporting (aligned to +/- strand): ref base G (27/57); new base A (2/3); total (29/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
GCAGGTTTCCGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAGCCTGAGCTTT > minE/2494671‑2494808 | gCAGGTTTCCGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCg > 1:1915762/1‑69 (MQ=255) cAGGTTTCCGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGag < 1:5170292/71‑1 (MQ=255) ggTTTCCGTCAGGAAGATTTAGAGGTTCAACTGGAAGTTACGCGCCTGAGCGTAAAAGGCACGCCGGagca < 1:3115060/71‑1 (MQ=255) ggTTTCCGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGagca < 1:2946870/71‑1 (MQ=255) tCCGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGa > 1:1490871/1‑64 (MQ=255) tCCGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGcc > 1:221048/1‑70 (MQ=255) tCCGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGcc > 1:4657710/1‑70 (MQ=255) cGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGTCTGAGCGTAAAAGGCACGCCg > 1:2388369/1‑60 (MQ=255) cGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCg > 1:3008449/1‑60 (MQ=255) cGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCg > 1:3443982/1‑60 (MQ=255) cGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCg > 1:356392/1‑60 (MQ=255) cGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCaa < 1:3253664/70‑1 (MQ=255) cGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCaa < 1:2005413/70‑1 (MQ=255) aGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAaaga < 1:520547/70‑1 (MQ=255) ggAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAaagaag < 1:2371914/71‑1 (MQ=255) aGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAagag < 1:2523902/70‑1 (MQ=255) gATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaa < 1:3777467/71‑1 (MQ=255) aTTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaaa < 1:933364/71‑1 (MQ=255) tttAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaaaa < 1:4155124/71‑1 (MQ=255) tttAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaaa < 1:341550/70‑1 (MQ=255) ttAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaaaa < 1:5200199/70‑1 (MQ=255) tAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaaaaaa > 1:1215449/1‑71 (MQ=255) tAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaaaaaa > 1:1486147/1‑71 (MQ=255) tAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaaaaaa > 1:928413/1‑71 (MQ=255) tAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaaaaaa > 1:1719016/1‑71 (MQ=255) tAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaaaaa < 1:3725606/70‑1 (MQ=255) agagATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaaaaaa < 1:5185937/70‑1 (MQ=255) aTTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAaag < 1:3503307/55‑1 (MQ=255) aTTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAaag < 1:3748126/55‑1 (MQ=255) ttCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGagc < 1:2331417/45‑1 (MQ=255) ttCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGagc < 1:3223198/45‑1 (MQ=255) ttCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGagc < 1:3745951/45‑1 (MQ=255) ttCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGagc < 1:3592519/45‑1 (MQ=255) ttCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGagc < 1:2018146/45‑1 (MQ=255) ttCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGagc < 1:3575128/45‑1 (MQ=255) ttCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGagc < 1:62480/45‑1 (MQ=255) tCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCAGAGCAGCCAAAAGAAGAGAAAAAATGGCTg > 1:3238596/1‑70 (MQ=255) tCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCAGAGCAGCCAAAAGAAGAGAAAAAATGGCTg > 1:743853/1‑70 (MQ=255) ccaCTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCaa < 1:3067773/47‑1 (MQ=255) aCTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATc < 1:3376735/71‑1 (MQ=255) cTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAaag < 1:2428532/49‑1 (MQ=255) cTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAaag < 1:1306302/49‑1 (MQ=255) cTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAaag < 1:3942048/49‑1 (MQ=255) ggAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAg > 1:3386047/1‑71 (MQ=255) ggAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAg > 1:1067716/1‑71 (MQ=255) ggAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAg > 1:4882035/1‑71 (MQ=255) ggAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAg > 1:780461/1‑71 (MQ=255) ggAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAg > 1:1284279/1‑71 (MQ=255) tACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCt < 1:249987/69‑1 (MQ=255) tACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCt < 1:243873/69‑1 (MQ=255) cgcgCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATg < 1:3110563/71‑1 (MQ=255) cgcgCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATg < 1:187063/71‑1 (MQ=255) gcgcCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGa < 1:4674491/71‑1 (MQ=255) gcgcCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGa < 1:621070/71‑1 (MQ=255) cgcCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCaa > 1:280896/1‑60 (MQ=255) cTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaaaaa < 1:2570243/44‑1 (MQ=255) aGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaa > 1:4460866/1‑38 (MQ=255) aGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaa > 1:279688/1‑38 (MQ=255) aGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaa > 1:2296435/1‑38 (MQ=255) aGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGaa > 1:1388745/1‑38 (MQ=255) aGCGTAAAAGGCACGCCAGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAgt < 1:941936/70‑2 (MQ=255) aGCGTAAAAGGCACGCCAGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAgt < 1:3722277/70‑2 (MQ=255) aGCGTAAAAGGCACGCCAGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAgt < 1:1117710/70‑2 (MQ=255) gCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCa > 1:4141386/1‑71 (MQ=255) tAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCAt < 1:347436/47‑1 (MQ=255) tAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCAt < 1:2837461/47‑1 (MQ=255) tAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCAt < 1:4580292/47‑1 (MQ=255) aaaaGGCACGCCGGAGCAGCCAAAAGAAGAGaaaa < 1:3210130/35‑1 (MQ=255) aaaaGGCACGCCGGAGCAGCCAAAAGAAGAGaaaa < 1:4105561/35‑1 (MQ=255) aaaaGGCACGCCGGAGCAGCCAAAAGAAGAGaaaa < 1:66572/35‑1 (MQ=255) aaaaGGCACGCCGGAGCAGCCAAAAGAAGAGaaaa < 1:4037941/35‑1 (MQ=255) aaaaGGCACGCCGGAGCAGCCAAAAGAAGAGaaaa < 1:3233645/35‑1 (MQ=255) aaaaGGCACGCCGGAGCAGCCAAAAGAAGAGaaaa < 1:2426877/35‑1 (MQ=255) aaGGCACGCCGGAGCAGCCAAAAGAAGAGaaaaaa > 1:1858710/1‑35 (MQ=255) aaGGCACGCCGGAGCAGCCAAAAGAAGAGaaaaaa > 1:2262646/1‑35 (MQ=255) aaGGCACGCCGGAGCAGCCAAAAGAAGAGaaaaaa > 1:2382710/1‑35 (MQ=255) aaGGCACGCCGGAGCAGCCAAAAGAAGAGaaaaaa > 1:5044150/1‑35 (MQ=255) aaGGCACGCCGGAGCAGCCAAAAGAAGAGaaaaaa > 1:502611/1‑35 (MQ=255) cGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAg < 1:896132/64‑1 (MQ=255) cGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAg < 1:453041/64‑1 (MQ=255) cGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAg < 1:1560397/64‑1 (MQ=255) cGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAg < 1:1478859/64‑1 (MQ=255) cGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAg < 1:2321581/64‑1 (MQ=255) cGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAg < 1:4168958/64‑1 (MQ=255) gCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAg < 1:4363975/63‑1 (MQ=255) gCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAGCCTGAGCt > 1:3471453/1‑71 (MQ=255) gCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAGCCTGAGCt > 1:4435152/1‑71 (MQ=255) ccGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGc < 1:1731759/55‑1 (MQ=255) ccGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGc < 1:5147152/55‑1 (MQ=255) ccGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAGCCTGAGCtt > 1:2402208/1‑71 (MQ=255) ccGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAGCCTGAGCtt > 1:2808587/1‑71 (MQ=255) cGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAGCCTGAGCttt < 1:2254699/71‑1 (MQ=255) cGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAGCCTGAGCttt < 1:1509346/71‑1 (MQ=255) cGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAGCCTGAGCtt < 1:3169558/70‑1 (MQ=255) | GCAGGTTTCCGTCAGGAAGATTTAGAGATTCAACTGGAAGGTACGCGCCTGAGCGTAAAAGGCACGCCGGAGCAGCCAAAAGAAGAGAAAAAATGGCTGCATCAAGGGCTTATGAATCAGCCATTTAGCCTGAGCTTT > minE/2494671‑2494808 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |