| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,545,265 | A→G | 5.7% | F78L (TTT→CTT) | rfaC ← | ADP‑heptose:LPS heptosyl transferase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,545,265 | 0 | A | G | 5.7% | 372.5 / 9.1 | 122 | F78L (TTT→CTT) | rfaC | ADP‑heptose:LPS heptosyl transferase I |
| Reads supporting (aligned to +/- strand): ref base A (35/80); new base G (2/5); total (37/85) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
CCGCGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGTATTGCCACAGGAAT > minE/2545196‑2545334 | ccgcGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAAc < 1:1621691/71‑1 (MQ=255)ccgcGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAAc < 1:4344104/71‑1 (MQ=255)ccgcGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAAc < 1:3979380/71‑1 (MQ=255)ccgcGCTTTTTACAAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAAc > 1:5088682/1‑71 (MQ=255) cgcgCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACg < 1:2466778/71‑1 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:2783851/71‑1 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:1150426/71‑1 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:1198068/71‑1 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:498238/71‑1 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:2125700/71‑1 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:2036999/71‑1 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:3759455/71‑1 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:3570713/71‑1 (MQ=255) tttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGTTTCGTGAAGCGCt < 1:1000565/68‑1 (MQ=255) tttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGTTTCGTGAAGCGCt < 1:4257384/68‑1 (MQ=255) tttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGTTTCGTGAAGCGCt < 1:4336245/68‑1 (MQ=255) tttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGTTTCGTGAAGCGCt < 1:1922041/68‑1 (MQ=255) tttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGTTTCGTGAAGCGCt < 1:4965671/68‑1 (MQ=255) ttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGc > 1:3616911/1‑71 (MQ=255) ttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGc > 1:4708153/1‑71 (MQ=255) tttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGTTTCGTGAAGCGCtac > 1:1482223/1‑66 (MQ=37) tttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCg > 1:4605049/1‑71 (MQ=255) ccAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTc < 1:550479/70‑1 (MQ=255) ccAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTc < 1:2505468/70‑1 (MQ=255) ccAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTc < 1:4963426/70‑1 (MQ=255) ccAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTc < 1:3365253/70‑1 (MQ=255) ccTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCtttt < 1:4574140/71‑1 (MQ=255) ccTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCtttt < 1:3628929/71‑1 (MQ=255) tGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:4996978/56‑1 (MQ=255) tGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:2176097/56‑1 (MQ=255) tGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:4081037/56‑1 (MQ=255) tGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:1905626/56‑1 (MQ=255) gAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATg > 1:2790271/1‑71 (MQ=255) gAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATg > 1:3432281/1‑71 (MQ=255) aGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTAt < 1:3329540/69‑1 (MQ=255) aGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTAt < 1:2647311/69‑1 (MQ=255) aGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATg < 1:542549/70‑1 (MQ=255) gATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGcc > 1:2596579/1‑70 (MQ=255) tAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag < 1:2421958/71‑1 (MQ=255) tAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag < 1:3017815/71‑1 (MQ=255) tAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag < 1:4275101/71‑1 (MQ=255) aaCTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTAt > 1:3659309/1‑60 (MQ=255) aaCTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATg > 1:3548593/1‑61 (MQ=255) aaCTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTAGCGTTCCGCTTTTAt > 1:4556396/1‑60 (MQ=255) aCTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGTTTTTATGGGGGCCGAGaa < 1:3869313/71‑1 (MQ=255) aCTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGaa < 1:1115527/71‑1 (MQ=255) tCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCttt < 1:3859431/50‑1 (MQ=255) aTAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCtt > 1:922801/1‑47 (MQ=255) aTAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCtt > 1:1462039/1‑47 (MQ=255) tAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt > 1:2320381/1‑70 (MQ=255) aGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa > 1:992030/1‑71 (MQ=255) aGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa > 1:1843934/1‑71 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCttt < 1:4479432/44‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCttt < 1:2736096/44‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCttt < 1:3931862/44‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTa < 1:5152062/46‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTa < 1:2908587/46‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTa < 1:1630178/46‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCATGCTTTa < 1:8017/69‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa < 1:129987/69‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa < 1:1980312/69‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa < 1:2244955/69‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa < 1:4216265/69‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa < 1:4158796/69‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa < 1:4720893/69‑1 (MQ=255) ctctGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGc > 1:1103572/1‑70 (MQ=255) gCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGc > 1:863079/1‑70 (MQ=255) cTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCg < 1:4155942/70‑1 (MQ=255) cTTGTAGCGCTTCACGAAACGATTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCg < 1:4631338/70‑1 (MQ=255) gTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACg > 1:2249930/1‑70 (MQ=255) gTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACg < 1:889786/70‑1 (MQ=255) tAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGt < 1:1387420/70‑1 (MQ=255) tAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGt < 1:4423790/70‑1 (MQ=255) aGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGt < 1:1412717/69‑1 (MQ=255) cgcTTCACGAAACGCTTTGCGTTCCTCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCTGCGACg < 1:4195220/66‑1 (MQ=255) cTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt < 1:4403758/51‑1 (MQ=255) cTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt < 1:2479647/51‑1 (MQ=255) cTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt < 1:4659195/51‑1 (MQ=255) cTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGCCCGAGAACCAGGCtt < 1:4432958/51‑1 (MQ=255) ttCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt < 1:5065098/50‑1 (MQ=255) ttCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt < 1:4027466/50‑1 (MQ=255) ttCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt < 1:25835/50‑1 (MQ=255) ttCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt < 1:865078/50‑1 (MQ=255) ttCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACg < 1:615741/63‑1 (MQ=255) ttCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACg < 1:3004122/63‑1 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGcc > 1:1089542/1‑36 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag > 1:1082024/1‑39 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag > 1:1209617/1‑39 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag > 1:2086242/1‑39 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag > 1:2954640/1‑39 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAgg > 1:1387069/1‑46 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa > 1:3884007/1‑51 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa > 1:2674269/1‑51 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa > 1:3322721/1‑51 (MQ=255) cGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt < 1:1149178/46‑1 (MQ=255) aaCGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGTATTGCCACAGGAAt < 1:1605771/71‑1 (MQ=255) aaCGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGTATTGCCACAGGAAt < 1:583885/71‑1 (MQ=255) aCGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGTATTGCCACAGGAAt > 1:1745758/1‑70 (MQ=255) aCGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGTATTGCCACAGGAAt > 1:653097/1‑70 (MQ=255) aCGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGTATTGCCACAGGAAt > 1:3218194/1‑70 (MQ=255) | CCGCGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGTATTGCCACAGGAAT > minE/2545196‑2545334 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |