Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,749 G→A 6.8% V283V (GTC→GTT arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,7490GA 6.8% 413.5 / 13.6 132V283V (GTC→GTTarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (59/64);  new base A (6/3);  total (65/67)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 135 total aligned reads displayed.

AAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACA  >  minE/2595683‑2595818
                                                                  |                                                                     
aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                                                   <  1:423141/71‑1 (MQ=255)
aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                                                   <  1:4489240/71‑1 (MQ=255)
aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                                                   <  1:4456247/71‑1 (MQ=255)
 aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                                  <  1:1029546/71‑1 (MQ=255)
    tatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTg                                                               >  1:2512693/1‑71 (MQ=255)
      ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                             <  1:1283160/71‑1 (MQ=255)
      ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                             <  1:470645/71‑1 (MQ=255)
       tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                                     >  1:219767/1‑62 (MQ=255)
       tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                                     >  1:3699924/1‑62 (MQ=255)
       tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                                     >  1:3954622/1‑62 (MQ=255)
       tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                                     >  1:2813726/1‑62 (MQ=255)
       tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                             <  1:3520395/70‑1 (MQ=255)
       tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATATGc                                                              >  1:2082750/1‑69 (MQ=255)
           ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                    <  1:4329728/59‑1 (MQ=255)
           ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                    <  1:657498/59‑1 (MQ=255)
           ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                    <  1:2745119/59‑1 (MQ=255)
                 cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                            >  1:3516885/1‑61 (MQ=255)
                 cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                            >  1:1190599/1‑61 (MQ=255)
                 cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                            >  1:110463/1‑61 (MQ=255)
                 cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGAGAGAAGATGACAATCTGCCa                                                            >  1:70461/1‑61 (MQ=255)
                  ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc                                                 <  1:4508988/71‑1 (MQ=255)
                  ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc                                                 <  1:736425/71‑1 (MQ=255)
                   gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                                  >  1:3807976/1‑53 (MQ=255)
                   gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                                  >  1:3783625/1‑53 (MQ=255)
                   gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                                  >  1:1684842/1‑53 (MQ=255)
                   gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                                  >  1:165568/1‑53 (MQ=255)
                   gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                                  >  1:4869153/1‑53 (MQ=255)
                   gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                                  >  1:3557356/1‑53 (MQ=255)
                        gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAgg                                            >  1:4477667/1‑70 (MQ=255)
                        gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                                           >  1:4760159/1‑71 (MQ=255)
                        gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                                           >  1:4208516/1‑71 (MQ=255)
                         cgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGAc                                          >  1:3986230/1‑71 (MQ=255)
                               ccATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                                     >  1:1058160/1‑38 (MQ=255)
                               ccATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                                     >  1:2076488/1‑38 (MQ=255)
                               ccATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                                     >  1:4901168/1‑38 (MQ=255)
                                cATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                    <  1:2543655/38‑1 (MQ=255)
                                cATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                    <  1:2160791/38‑1 (MQ=255)
                                cATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                    <  1:4741266/38‑1 (MQ=255)
                                cATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                    <  1:1837648/38‑1 (MQ=255)
                                cATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                    <  1:3779034/38‑1 (MQ=255)
                                cATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                                    <  1:1058710/38‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                             <  1:2643766/43‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                             <  1:2655549/43‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                              <  1:4839466/42‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                              <  1:792798/42‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                              <  1:944278/42‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                              <  1:3072604/42‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                              <  1:2183705/42‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                              <  1:2890437/42‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc                                                    <  1:1114187/52‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc                                                    <  1:1278359/52‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc                                                    <  1:5053945/52‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc                                                    <  1:1640059/52‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc                                                    <  1:4425164/52‑1 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc                                   >  1:3169769/1‑69 (MQ=255)
                                  tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCg                                  >  1:302012/1‑70 (MQ=255)
                                    aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                            >  1:1368904/1‑42 (MQ=255)
                                    aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                                          >  1:1564081/1‑44 (MQ=255)
                                    aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                                          >  1:1855670/1‑44 (MQ=255)
                                    aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                                          >  1:3203780/1‑44 (MQ=255)
                                    aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                                          >  1:4245624/1‑44 (MQ=255)
                                    aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                                          >  1:2717454/1‑44 (MQ=255)
                                    aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa                                <  1:489454/70‑1 (MQ=255)
                                    aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAt                               >  1:3996750/1‑71 (MQ=255)
                                    aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAt                               >  1:824228/1‑71 (MQ=255)
                                    aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAt                               >  1:1811029/1‑71 (MQ=255)
                                           aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                         >  1:471589/1‑70 (MQ=255)
                                           aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc                        <  1:4850556/71‑1 (MQ=255)
                                            gATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCa                       <  1:5047097/71‑1 (MQ=255)
                                              tACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATg                     <  1:3476054/71‑1 (MQ=255)
                                              tACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGAAt                      <  1:196274/70‑1 (MQ=255)
                                               aCATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa                    >  1:2745877/1‑71 (MQ=255)
                                                 aTGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                                   >  1:2449413/1‑38 (MQ=255)
                                                 aTGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                                   >  1:2969399/1‑38 (MQ=255)
                                                 aTGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                                   >  1:2365532/1‑38 (MQ=255)
                                                  tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcct                 >  1:3760988/1‑70 (MQ=255)
                                                  tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcct                 >  1:2363060/1‑70 (MQ=255)
                                                  tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcct                 >  1:2643713/1‑70 (MQ=255)
                                                  tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcct                 >  1:4590037/1‑70 (MQ=255)
                                                  tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcc                  >  1:2919275/1‑70 (MQ=255)
                                                        gtgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCttttc           <  1:2800717/69‑2 (MQ=255)
                                                        gtgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCttttc           <  1:3026550/69‑2 (MQ=255)
                                                        gtgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCttttc           <  1:4383066/69‑2 (MQ=255)
                                                          gagaAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                         <  1:1917654/55‑1 (MQ=255)
                                                          gagaAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                         <  1:4328686/55‑1 (MQ=255)
                                                           agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                             <  1:2224188/50‑1 (MQ=255)
                                                           agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                             <  1:786577/50‑1 (MQ=255)
                                                            gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCa                       >  1:1095870/1‑55 (MQ=255)
                                                            gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCa                       >  1:3299263/1‑55 (MQ=255)
                                                             aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg               <  1:3255475/62‑1 (MQ=255)
                                                             aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg               <  1:5078090/62‑1 (MQ=255)
                                                             aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg               <  1:2933304/62‑1 (MQ=255)
                                                             aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg               <  1:3610010/62‑1 (MQ=255)
                                                             aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCCTATTAATCGCATGACcgcg               <  1:728229/62‑1 (MQ=255)
                                                              agaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                            >  1:2548385/1‑48 (MQ=255)
                                                               gaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt             >  1:4425836/1‑62 (MQ=255)
                                                                 tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa  <  1:3021015/71‑1 (MQ=255)
                                                                  gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa  >  1:1406680/1‑70 (MQ=255)
                                                                  gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa  >  1:4341645/1‑70 (MQ=255)
                                                                  gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa  >  1:3648213/1‑70 (MQ=255)
                                                                  |                                                                     
AAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACA  >  minE/2595683‑2595818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: