| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 168,689 | G→T | 13.7% | intergenic (‑358/‑10) | map ← / → rpsB | methionine aminopeptidase/30S ribosomal subunit protein S2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 168,689 | 0 | G | T | 13.7% | 496.6 / 39.1 | 159 | intergenic (‑358/‑10) | map/rpsB | methionine aminopeptidase/30S ribosomal subunit protein S2 |
| Reads supporting (aligned to +/- strand): ref base G (81/56); new base T (14/8); total (95/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
Only 100 of 162 total aligned reads displayed.
CCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCC > minE/168624‑168759 | ccGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGttt > 1:4881138/1‑70 (MQ=255)ccGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGttt > 1:4114337/1‑70 (MQ=255) cGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTa < 1:4581833/71‑1 (MQ=255) gggTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAAt < 1:4132246/71‑1 (MQ=255) gTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCa < 1:3426222/71‑1 (MQ=255) cccTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTaa > 1:2852746/1‑65 (MQ=255) cccTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTaa > 1:1616854/1‑65 (MQ=255) tGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAAc < 1:1175690/70‑1 (MQ=255) tGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAAc < 1:2848966/70‑1 (MQ=255) tGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt > 1:2466217/1‑71 (MQ=255) gggTCGGTAATATGGGATACGTGGAGGCATAACTCCAACTTTTATATAGAGGtttt < 1:3083878/56‑1 (MQ=255) gggTCGGTAATATGGGATACGTGGAGGCATAACTCCAACTTTTATATAGAGGtttt < 1:714316/56‑1 (MQ=255) gggTCGGTAATATGGGATACGTGGAGGCATAACTCCAACTTTTATATAGAGGtttt < 1:2606077/56‑1 (MQ=255) cGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTc > 1:3489493/1‑70 (MQ=255) ggTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa > 1:985743/1‑71 (MQ=255) ggTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa > 1:5181509/1‑71 (MQ=255) ggTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa > 1:1460973/1‑71 (MQ=255) tAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCaa < 1:2202933/60‑1 (MQ=255) tGGGATAGGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGa < 1:3691994/71‑1 (MQ=255) tGGGATACGTGGAGGCATATCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGa > 1:3280932/1‑71 (MQ=255) tGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGa > 1:4306886/1‑71 (MQ=255) tGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGa > 1:1667365/1‑71 (MQ=255) tGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGa > 1:1470981/1‑71 (MQ=255) tGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGa > 1:409097/1‑71 (MQ=255) ggATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAAt < 1:2364909/45‑1 (MQ=255) ggATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAAt < 1:1854306/45‑1 (MQ=255) ggATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAAt < 1:4880860/45‑1 (MQ=255) ggATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAAt < 1:378788/45‑1 (MQ=255) gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt < 1:2382143/44‑1 (MQ=255) gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt < 1:1585636/44‑1 (MQ=255) gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt < 1:3624408/44‑1 (MQ=255) gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt < 1:3793862/44‑1 (MQ=255) gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt < 1:3857817/44‑1 (MQ=255) gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt < 1:4728720/44‑1 (MQ=255) gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt < 1:2039467/44‑1 (MQ=255) tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa > 1:3493460/1‑44 (MQ=255) tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa > 1:4130693/1‑44 (MQ=255) tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa > 1:98085/1‑44 (MQ=255) tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa > 1:4181778/1‑44 (MQ=255) tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa > 1:3590051/1‑44 (MQ=255) tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa > 1:162240/1‑44 (MQ=255) tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa > 1:2494272/1‑44 (MQ=255) tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa > 1:1317931/1‑44 (MQ=255) cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa > 1:4821762/1‑57 (MQ=255) cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa > 1:5073019/1‑57 (MQ=255) cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa > 1:5159843/1‑57 (MQ=255) cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCt > 1:4387552/1‑70 (MQ=255) tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCTACt < 1:626943/48‑1 (MQ=255) tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt < 1:4160/48‑1 (MQ=255) tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt < 1:1858292/48‑1 (MQ=255) tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt < 1:4588854/48‑1 (MQ=255) tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt < 1:3824317/48‑1 (MQ=255) tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt < 1:3600942/48‑1 (MQ=255) tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt < 1:2764593/48‑1 (MQ=255) ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCa > 1:2884766/1‑39 (MQ=255) ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCa > 1:3906611/1‑39 (MQ=255) ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCa > 1:1187496/1‑39 (MQ=255) ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAg > 1:2594115/1‑71 (MQ=255) ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAg > 1:570274/1‑71 (MQ=255) ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAg > 1:854746/1‑71 (MQ=255) ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAg > 1:979996/1‑71 (MQ=255) ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAg > 1:4545925/1‑71 (MQ=255) ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAg > 1:2152434/1‑71 (MQ=255) gAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAgg < 1:356747/71‑1 (MQ=255) ggCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCt < 1:876118/71‑1 (MQ=255) aTAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGgt > 1:323203/1‑71 (MQ=255) tAACCCCAACTTTTATATAGAGGTTTTAATCATGGc < 1:4491042/36‑1 (MQ=255) tAACCCCAACTTTTATATAGAGGTTTTAATCATGGc < 1:1125778/36‑1 (MQ=255) aaCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATgcg > 1:127083/1‑50 (MQ=255) aaCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATgcg > 1:4804846/1‑50 (MQ=255) aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa > 1:2901590/1‑45 (MQ=255) aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAgg > 1:3000168/1‑63 (MQ=255) aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAgg > 1:2036783/1‑63 (MQ=255) aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAgg > 1:4111161/1‑63 (MQ=255) aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGgtgt > 1:2014862/1‑70 (MQ=255) aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGgtgt > 1:133109/1‑70 (MQ=255) aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt > 1:3303009/1‑71 (MQ=255) aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt > 1:217675/1‑71 (MQ=255) aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt < 1:838952/71‑1 (MQ=255) aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt > 1:2475077/1‑71 (MQ=255) aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt > 1:1034209/1‑71 (MQ=255) ccccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTc < 1:179770/71‑1 (MQ=255) ccccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTc < 1:3408116/71‑1 (MQ=255) ccccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTc < 1:3414316/71‑1 (MQ=255) ccccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTc < 1:433620/71‑1 (MQ=255) ccccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTc < 1:366308/71‑1 (MQ=255) cccAACTTTTATATAGAGGTTTTTATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGc < 1:2268445/62‑1 (MQ=255) cccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGc < 1:4068464/62‑1 (MQ=255) cccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGc < 1:4008056/62‑1 (MQ=255) cccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGc < 1:423622/62‑1 (MQ=255) ccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATg > 1:4559737/1‑53 (MQ=255) ccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATg > 1:738024/1‑53 (MQ=255) ccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATg > 1:1608259/1‑53 (MQ=255) cAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGgt < 1:4166097/64‑1 (MQ=255) aaCTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt < 1:4809969/66‑1 (MQ=255) aaCTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCT‑AAGGCTGGTGTTCACTTc > 1:4313338/1‑71 (MQ=255) tttATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGt > 1:2179844/1‑71 (MQ=255) ttATATAGAGGTTTTAATCATGGCAACTGTTTCCa > 1:545977/1‑35 (MQ=255) ttATATAGAGGTTTTAATCATGGCAACTGTTTCCa > 1:2982399/1‑35 (MQ=255) ggTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGAccc > 1:2219449/1‑71 (MQ=255) | CCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCC > minE/168624‑168759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |