| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,682,425 | A→T | 14.8% | A429A (GCT→GCA) | yhgF ← | predicted transcriptional accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,682,425 | 0 | A | T | 14.8% | 213.0 / 27.7 | 81 | A429A (GCT→GCA) | yhgF | predicted transcriptional accessory protein |
| Reads supporting (aligned to +/- strand): ref base A (44/25); new base T (8/4); total (52/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
GATCCTGCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTGCGCGGTCAC > minE/2682355‑2682490 | gATCCTGCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGca < 1:2608787/71‑1 (MQ=255)gATCCTGCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGca < 1:4288474/71‑1 (MQ=255)gATCCTGCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGca < 1:3855165/71‑1 (MQ=255)gATCCTGCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGca < 1:4375506/71‑1 (MQ=255) cTGCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGcat < 1:4551221/68‑2 (MQ=255) gCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGcagccagc > 1:1285544/1‑71 (MQ=255) gCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGcagccagc > 1:1422299/1‑71 (MQ=255) gCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGcagccagc > 1:3874243/1‑71 (MQ=255) aaaCGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCt > 1:4725042/1‑70 (MQ=255) ggcgATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGcc > 1:2407914/1‑70 (MQ=255) cgATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAg > 1:621769/1‑71 (MQ=255) cgATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAg > 1:5190736/1‑71 (MQ=255) aTAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGt < 1:1510798/70‑1 (MQ=255) aTAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGt < 1:1950570/70‑1 (MQ=255) aTAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTa < 1:5055462/71‑1 (MQ=255) aGACACCGCGCCACGCAGCGTAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTa > 1:2054650/1‑69 (MQ=255) aGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTaaa > 1:4538035/1‑71 (MQ=255) aGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTa > 1:3807170/1‑69 (MQ=255) acacCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAAcc < 1:2372462/71‑1 (MQ=255) acacCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAAcc < 1:3422261/71‑1 (MQ=255) acacCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAAcc < 1:78670/71‑1 (MQ=255) cgcgCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGc > 1:197291/1‑57 (MQ=255) cgcgCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGc > 1:2557391/1‑57 (MQ=255) cgcgCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGc > 1:1369766/1‑57 (MQ=255) cgcgCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGc > 1:1307376/1‑57 (MQ=255) cgcgCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGc > 1:781550/1‑57 (MQ=255) cgcgCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGc > 1:1153278/1‑57 (MQ=255) cgcgCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGc > 1:4558343/1‑57 (MQ=255) cgcCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGAcgcg < 1:2625832/71‑1 (MQ=255) gCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCg < 1:2864473/44‑1 (MQ=255) gCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCg < 1:3452871/44‑1 (MQ=255) gCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGa > 1:399412/1‑61 (MQ=255) gCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGa > 1:729798/1‑61 (MQ=255) gCAGCGAAACGTCGAGATCCGGAAATTCCTGTGCTGCCAGCTCGGa < 1:3673476/46‑1 (MQ=38) gCAGCGAAACGTCGAGATCCGGAAATTCCTGTGCTGCCAGCTCGGa < 1:2332545/46‑1 (MQ=38) gCAGCGAAACGTCGAGATCCGGAAATTCCTGTGCTGCCAGCTCGGa < 1:1251784/46‑1 (MQ=38) gCAGCGAAACGTCGAGATCCGGAAATTCCTGTGCTGCCAGCTCGGa < 1:4808405/46‑1 (MQ=38) cAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCtt > 1:4575444/1‑71 (MQ=255) cAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCtt > 1:557806/1‑71 (MQ=255) aGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTc < 1:2755289/71‑1 (MQ=255) gCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCg < 1:2126403/71‑1 (MQ=255) gCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCg < 1:1465475/71‑1 (MQ=255) gAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCt > 1:5074013/1‑71 (MQ=255) gAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCt > 1:326606/1‑71 (MQ=255) aaaCGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCt > 1:1850092/1‑70 (MQ=255) aaaCGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCt > 1:3593262/1‑70 (MQ=255) aaaCGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCt > 1:1597236/1‑70 (MQ=255) aaaCGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCt > 1:421967/1‑70 (MQ=255) aCGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGa > 1:543794/1‑53 (MQ=255) aCGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGa > 1:3829915/1‑53 (MQ=255) aCGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGa > 1:5064502/1‑53 (MQ=255) aCGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGa > 1:4936372/1‑53 (MQ=255) aCGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGa > 1:4904284/1‑53 (MQ=255) aCGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGa > 1:970074/1‑53 (MQ=255) aCGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGa > 1:3382563/1‑53 (MQ=255) aCGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGa > 1:2728664/1‑53 (MQ=255) aCGTCGAGATCCGGAAATTCCTGTGCTGCCAGCTCGGaa > 1:1725561/1‑39 (MQ=25) aCGTCGAGATCCGGAAATTCCTGTGCTGCCAGCTCGGAAg > 1:2524166/1‑40 (MQ=37) aCGTCGAGATCCGGAAATTCCTGTGCTGCCAGCTCGGAAg > 1:1185155/1‑40 (MQ=37) aCGTCGAGATCCGGAAATTCCTGTGCTGCCAGCTCGGAAg > 1:1634558/1‑40 (MQ=37) aCGTCGAGATCCGGAAATTCCTGTGCTGCCAGCTCGGAAg > 1:536324/1‑40 (MQ=37) aCGTCGAGATCCGGAAATTCCTGTGCTGCCAGCTCGGAAg > 1:1786186/1‑40 (MQ=37) aCGTCGAGATCCGGAAATTCCTGTGCTGCCAGCTCGGAAg > 1:4770040/1‑40 (MQ=37) aCGTCGAGATCCGGAAATTCCTGTGCTGCCAGCTCGGAAg > 1:2827550/1‑40 (MQ=37) gaTCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACt < 1:4782023/71‑1 (MQ=255) aTCCGTGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACtt < 1:3438560/71‑1 (MQ=255) aTCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACt < 1:4451179/70‑1 (MQ=255) aTCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACt < 1:118950/70‑1 (MQ=255) aTCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACt < 1:548699/70‑1 (MQ=255) aTCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACt < 1:4725795/70‑1 (MQ=255) aTCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACt < 1:3990698/70‑1 (MQ=255) tCCGGTAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACt < 1:3129299/69‑1 (MQ=255) aaCTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTgcgc > 1:1982520/1‑71 (MQ=255) aaCTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTgcgc > 1:690492/1‑71 (MQ=255) aaCTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTgcgc > 1:3871161/1‑71 (MQ=255) aCTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTgc > 1:2935125/1‑68 (MQ=255) aCTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTgc > 1:3151731/1‑68 (MQ=255) cTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGc > 1:12062/1‑47 (MQ=255) cTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTGCGCGGTc < 1:3844649/70‑1 (MQ=255) cTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTGCGCGGTc > 1:193334/1‑70 (MQ=255) cTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTGCGCGGTCa > 1:2149280/1‑71 (MQ=255) cTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTGCGCGGTCa > 1:1695453/1‑71 (MQ=255) cTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTGCGCGGTCa > 1:5139668/1‑71 (MQ=255) cTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTGCGCGGTCa > 1:511594/1‑71 (MQ=255) cTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTGCGCGGTCa > 1:2033298/1‑71 (MQ=255) tgCAGCCAGCTCGGAAGCCGAGTAAACCGACGCTCCTGCTTCGCTGACGAtt < 1:3881964/52‑1 (MQ=255) tgCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGAtt < 1:2733009/52‑1 (MQ=255) tgCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGAtt < 1:721410/52‑1 (MQ=255) tgCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTGCGCGGTCAc < 1:2841561/69‑1 (MQ=255) cagccagcTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTc > 1:1478374/1‑56 (MQ=255) cagccagcTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCt > 1:3141920/1‑57 (MQ=255) cagccagcTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCt > 1:2762835/1‑57 (MQ=255) cagccagcTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCt > 1:4985629/1‑57 (MQ=255) cagccagcTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCt > 1:3135860/1‑57 (MQ=255) cagccagcTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCt > 1:2080400/1‑57 (MQ=255) cagccagcTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCt > 1:803582/1‑57 (MQ=255) cagccagcTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCt > 1:3498070/1‑57 (MQ=255) | GATCCTGCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTCGAGATCCGGGAACTCCTGTGCAGCCAGCTCGGAAGCCGAGTAAACCGACGCGCCTGCTTCGCTGACGATTACTTTCTGCGCGGTCAC > minE/2682355‑2682490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |