| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,714,529 | T→C | 5.8% | intergenic (‑169/+93) | nirB ← / ← tsgA | nitrite reductase, large subunit, NAD(P)H‑binding/predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,714,529 | 0 | T | C | 5.8% | 361.8 / 9.2 | 120 | intergenic (‑169/+93) | nirB/tsgA | nitrite reductase, large subunit, NAD(P)H‑binding/predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (45/68); new base C (4/3); total (49/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
TATTGCTGATTTGTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCGGGGGAAGAGAGGAAAAGGCCGGACGA > minE/2714462‑2714596 | tatTGCTGATTTGTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGa > 1:1587043/1‑71 (MQ=255) ttGCTGATTTGTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACa < 1:2398634/71‑1 (MQ=255) ttGCTGATTTGTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACa < 1:1991517/71‑1 (MQ=255) tGCTGATTTGTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAACAGAc > 1:3049344/1‑69 (MQ=255) tGCTGATTTGTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAACAGACa > 1:3043221/1‑70 (MQ=255) tGCTGATTTGTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAACAGACa > 1:1549968/1‑70 (MQ=255) cTGATTTGTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACaaa < 1:4423195/70‑1 (MQ=255) gATTTGTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATa > 1:82373/1‑62 (MQ=255) gATTTGTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATa > 1:3221511/1‑62 (MQ=255) gATTTGTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATa > 1:3924936/1‑62 (MQ=255) gTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACaaaa < 1:459061/64‑1 (MQ=255) gTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACaaaa < 1:2302371/64‑1 (MQ=255) gTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACaaaa < 1:29189/64‑1 (MQ=255) gTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACaaaa < 1:5102165/64‑1 (MQ=255) gTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACaaaa < 1:703018/64‑1 (MQ=255) gTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACaaaa < 1:1224676/64‑1 (MQ=255) gTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACaaaa < 1:1005441/64‑1 (MQ=255) tataAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACaaaa < 1:2376465/63‑1 (MQ=255) taAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATa < 1:4273115/54‑1 (MQ=255) taAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATa < 1:3337956/54‑1 (MQ=255) taAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATa < 1:4830975/54‑1 (MQ=255) taAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATa < 1:584139/54‑1 (MQ=255) taAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATa < 1:872447/54‑1 (MQ=255) aaaTTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGtt > 1:780872/1‑70 (MQ=255) aaaTTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGtt > 1:1199828/1‑70 (MQ=255) aaaTTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGtt > 1:5098296/1‑70 (MQ=255) aaTTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTta > 1:2631879/1‑71 (MQ=255) aTTTTCTTAAGTCACGGAATTGTCTTATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTtat < 1:4095157/71‑1 (MQ=255) aTTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTtat < 1:625432/71‑1 (MQ=255) aTTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTta < 1:2078299/70‑1 (MQ=255) ttttCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTta > 1:145795/1‑69 (MQ=255) ttttCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTta > 1:2931566/1‑69 (MQ=255) ttCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAg > 1:2704052/1‑70 (MQ=255) tCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGa < 1:2247797/70‑1 (MQ=255) cTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACa < 1:4319967/50‑1 (MQ=255) ttAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGa < 1:1626824/68‑1 (MQ=255) ttAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGa < 1:3185495/68‑1 (MQ=255) aaGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGaaa > 1:446384/1‑68 (MQ=255) aaGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGaaa > 1:4785879/1‑68 (MQ=255) aaGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATa < 1:3928049/70‑1 (MQ=255) aaGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAACAGACAAATTGAACGGATTTATAGAAAt < 1:4233674/69‑1 (MQ=255) aaGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAACAGACAAATTGAACGGATTTATAGAAAt < 1:527353/69‑1 (MQ=255) aGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAACAGACAAATTGAACGGATTTATAGAAAt < 1:1343731/68‑1 (MQ=255) cACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATg > 1:1097987/1‑70 (MQ=255) cACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATg > 1:3583697/1‑70 (MQ=255) aCGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCa > 1:4109444/1‑71 (MQ=255) aCGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCa > 1:1869199/1‑71 (MQ=255) aCGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCa > 1:3627135/1‑71 (MQ=255) aCGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCa > 1:5125548/1‑71 (MQ=255) cGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATa < 1:3589143/64‑1 (MQ=255) gTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAg < 1:419053/41‑1 (MQ=255) gTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAg < 1:1994701/41‑1 (MQ=255) tCTGATATTTCATGTTTGTGCAAAAAACAGACAAATTGAACGGATTTATAGAAATAGATGTACGTAGca > 1:2145529/1‑68 (MQ=255) cagATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCg > 1:2823971/3‑41 (MQ=255) cTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCg > 1:2449406/1‑41 (MQ=255) cTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCg > 1:4687517/1‑41 (MQ=255) cTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCg > 1:1528026/1‑41 (MQ=255) cTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCg > 1:3379668/1‑41 (MQ=255) tGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGa < 1:3590145/50‑1 (MQ=255) tGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGa < 1:1796793/50‑1 (MQ=255) tGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGa < 1:677161/50‑1 (MQ=255) tGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGa < 1:2067434/50‑1 (MQ=255) tGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGa < 1:584772/50‑1 (MQ=255) tGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGa < 1:1546848/50‑1 (MQ=255) tGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGa < 1:3405082/50‑1 (MQ=255) atatTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGaaa > 1:2323910/1‑50 (MQ=255) atatTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGaaa > 1:2658299/1‑50 (MQ=255) atatTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCggggg > 1:1688086/1‑69 (MQ=255) atatTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCgggg < 1:2880430/68‑1 (MQ=255) tatTTCATGTTTGTGCAAAAAATAGACAAAATGAGCggg < 1:4611767/39‑1 (MQ=255) tatTTCATGTTTGTGCAAAAAATAGACAAAATGAGCggg < 1:3794167/39‑1 (MQ=255) tatTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCgggg < 1:421492/67‑1 (MQ=255) tttCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCa < 1:4390839/55‑1 (MQ=255) tttCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCa < 1:44559/55‑1 (MQ=255) tttCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCa < 1:4572239/55‑1 (MQ=255) tttCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCa < 1:1605860/55‑1 (MQ=255) tttCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCa < 1:795884/55‑1 (MQ=255) tttCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCGGGGGaa > 1:4870445/1‑68 (MQ=255) ttCATGTTTGTGCAAAAAATAGACAAAATGAGCggg < 1:3315137/36‑1 (MQ=255) ttCATGTTTGTGCAAAAAATAGACAAAATGAGCggg < 1:3938877/36‑1 (MQ=255) ttCATGTTTGTGCAAAAAATAGACAAAATGAGCggg < 1:1711758/36‑1 (MQ=255) ttCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATa < 1:2855467/48‑1 (MQ=255) ttCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATa < 1:2739806/48‑1 (MQ=255) tCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGa > 1:2973002/1‑49 (MQ=255) tCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGa > 1:3530760/1‑49 (MQ=255) tGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCGGGGGAAGAGAGGaa < 1:1978961/71‑1 (MQ=255) gTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGaaa > 1:3129440/1‑41 (MQ=255) tttGTGCAAAAAATAGACAAATTGAGCGGGTTTATAGAAATAGATGCACGTAGCGGGGGAAGAGAGGa < 1:2252286/68‑1 (MQ=255) tttGTGCAAAAAATAGACAAAATGAGCGGGTTtat > 1:876238/1‑35 (MQ=255) tttGTGCAAAAAATAGACAAAATGAGCGGGTTtat > 1:5106094/1‑35 (MQ=255) tttGTGCAAAAAATAGACAAAATGAGCGGGTTtat > 1:3980680/1‑35 (MQ=255) tttGTGCAAAAAATAGACAAAATGAGCGGGTTtat > 1:3369353/1‑35 (MQ=255) tttGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCGGGGGAAGAGAGGaaa < 1:2612644/70‑1 (MQ=255) tttGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCGGGGGAAGAGAGGa < 1:3313059/68‑1 (MQ=255) tgtgCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCGGGGGAAGAGAGGAAAAg < 1:2590098/70‑1 (MQ=255) cAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCGGGGGAAGAGAGGAAAAGGCCg < 1:3927442/70‑1 (MQ=255) cAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCGGGGGAAGAGAGGAAAAGGCCg < 1:1000436/70‑1 (MQ=255) cAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCGGGGGAAGAGAGGAAAAGGCCg > 1:3139647/1‑70 (MQ=255) aaaTAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCGGGGGAAGAGAGGAAAAGGCCGgacga > 1:4704787/1‑71 (MQ=255) aaaTAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCGGGGGAAGAGAGGAAAAGGCCGgacga > 1:4395274/1‑71 (MQ=255) | TATTGCTGATTTGTATAAATTTTCTTAAGTCACGGAATTGTCTGATATTTCATGTTTGTGCAAAAAATAGACAAAATGAGCGGGTTTATAGAAATAGATGCACGTAGCGGGGGAAGAGAGGAAAAGGCCGGACGA > minE/2714462‑2714596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |