| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 168,776 | A→G | 16.5% | K26K (AAA→AAG) | rpsB → | 30S ribosomal subunit protein S2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 168,776 | 0 | A | G | 16.5% | 199.9 / 29.8 | 85 | K26K (AAA→AAG) | rpsB | 30S ribosomal subunit protein S2 |
| Reads supporting (aligned to +/- strand): ref base A (35/36); new base G (6/8); total (41/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
TGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATG > minE/168707‑168845 | tGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGaaaa > 1:570678/1‑71 (MQ=255) gTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAAt > 1:2691611/1‑71 (MQ=255) gTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAAt > 1:3082617/1‑71 (MQ=255) gTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAAt > 1:1477803/1‑71 (MQ=255) tttCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAAt < 1:4877715/70‑1 (MQ=255) gcgaGACATGCTCAAGGCTGGTGTTAACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGt > 1:1938177/1‑71 (MQ=255) cgcgACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGtt > 1:2080191/1‑71 (MQ=255) cgcgACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGtt > 1:331490/1‑71 (MQ=255) cgACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCa > 1:2351522/1‑71 (MQ=255) cgACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCa > 1:3721297/1‑71 (MQ=255) aTGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCtt < 1:3924032/71‑1 (MQ=255) tGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTc < 1:4992802/71‑1 (MQ=255) cTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCgg < 1:4024852/71‑1 (MQ=255) cTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCgg < 1:3843249/71‑1 (MQ=255) cTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCgg < 1:181749/71‑1 (MQ=255) ggCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCg > 1:4026613/1‑65 (MQ=255) tgtTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAAct < 1:2321346/71‑2 (MQ=255) tgtTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACa < 1:1047860/71‑1 (MQ=255) cACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGt > 1:2150660/1‑71 (MQ=255) aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt < 1:3635253/71‑1 (MQ=255) aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt < 1:2762509/71‑1 (MQ=255) aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt < 1:4640291/71‑1 (MQ=255) aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt < 1:3984658/71‑1 (MQ=255) aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt < 1:4006017/71‑1 (MQ=255) aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt < 1:2827670/71‑1 (MQ=255) aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt < 1:925167/71‑1 (MQ=255) aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt < 1:4969061/71‑1 (MQ=255) cGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCtt > 1:1425125/1‑48 (MQ=255) tCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt < 1:3640484/64‑1 (MQ=255) ccACCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCGGtc < 1:2411383/49‑2 (MQ=255) ccACCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCGGtc < 1:3183768/49‑2 (MQ=255) ccACCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCGGtc < 1:3278474/49‑2 (MQ=255) ccACCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCGGtc < 1:3691794/49‑2 (MQ=255) ccACCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCGGtc < 1:4473186/49‑2 (MQ=255) aCCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCg < 1:4117064/45‑1 (MQ=255) aCCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCg < 1:345409/45‑1 (MQ=255) aCCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCg < 1:4524942/45‑1 (MQ=255) aCCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACatcatc < 1:1405846/71‑1 (MQ=255) aGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAAc > 1:4709798/1‑71 (MQ=255) aGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAAc > 1:3284444/1‑71 (MQ=255) ttACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatca < 1:5164079/59‑1 (MQ=255) ttACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTg < 1:3305117/68‑1 (MQ=255) ttACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTg < 1:1815441/68‑1 (MQ=255) ttACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTg < 1:619016/68‑1 (MQ=255) ttACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTg < 1:949097/68‑1 (MQ=255) ttcTGGAACCCAAAGATGGCGCCGTTCATCTTCGGTCATCGCAACAAGATCCACATCATCAACCTGgaa > 1:3345596/3‑68 (MQ=255) ttcTGGAACCCAAAGATGGCGCCGTTCATCTTCGGTCATCGCAACAAGATCCACATCATCAACCTGgaa > 1:1957858/3‑68 (MQ=255) ttcTGGAACCCAAAGATGGCGCCGTTCATCTTCGGTCATCGCAACAAGATCCACATCATCAACCTGgaa > 1:3153511/3‑68 (MQ=255) ttcTGGAACCCAAAGATGGCGCCGTTCATCTTCGGTCATCGCAACAAGATCCACATCATCAACCTGgaa > 1:613621/3‑68 (MQ=255) ttcTGGAACCCAAAGATGGCGCCGTTCATCTTCGGTCATCGCAACAAGATCCACATCATCAACCTGgaa > 1:3981477/3‑68 (MQ=255) ttcTGGAACCCAAAGATGGCGCCGTTCATCTTCGGTCATCGCAACAAGATCCACATCATCAACCTGgaa > 1:3975713/3‑68 (MQ=255) aCTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAAc > 1:4794908/1‑62 (MQ=255) aCTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaa > 1:4375234/1‑70 (MQ=255) aCTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaa > 1:4675061/1‑70 (MQ=255) gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc < 1:994371/69‑1 (MQ=255) gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc < 1:2002149/69‑1 (MQ=255) gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc < 1:3781457/69‑1 (MQ=255) gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc < 1:3776523/69‑1 (MQ=255) gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc < 1:2006176/69‑1 (MQ=255) gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc < 1:2231271/69‑1 (MQ=255) gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTg > 1:2662749/1‑71 (MQ=255) gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTg > 1:3133820/1‑71 (MQ=255) gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTg > 1:3621608/1‑71 (MQ=255) gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTg > 1:3371208/1‑71 (MQ=255) aaCCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaaa < 1:3869276/66‑1 (MQ=255) aaCCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaaa < 1:4648313/66‑1 (MQ=255) aaCCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGt < 1:3921620/71‑1 (MQ=255) aaCCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGt < 1:4215555/71‑1 (MQ=255) aaCCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGt < 1:591818/71‑1 (MQ=255) aCCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGt < 1:2549488/70‑1 (MQ=255) cccGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTa > 1:2329402/1‑70 (MQ=255) cccGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTa > 1:1064246/1‑70 (MQ=255) cccGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTa > 1:3796152/1‑70 (MQ=255) gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc > 1:2604352/1‑46 (MQ=255) gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc > 1:4916655/1‑46 (MQ=255) gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc > 1:433530/1‑46 (MQ=255) gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc > 1:5056753/1‑46 (MQ=255) gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc > 1:3793428/1‑46 (MQ=255) gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc > 1:1653585/1‑46 (MQ=255) gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc > 1:1522768/1‑46 (MQ=255) gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc > 1:1159104/1‑46 (MQ=255) gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc > 1:3954565/1‑46 (MQ=255) gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACatcatc < 1:757374/49‑1 (MQ=255) gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACatcatc > 1:945975/1‑49 (MQ=255) gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGa > 1:3355882/1‑71 (MQ=255) gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGa > 1:1157110/1‑71 (MQ=255) aaaTGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATg > 1:4179055/1‑71 (MQ=255) aaTGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc < 1:1896171/60‑1 (MQ=255) | TGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATG > minE/168707‑168845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |