| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,880,895 | C→T | 8.4% | L71L (TTG→TTA) | ytfF ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,880,895 | 0 | C | T | 8.4% | 468.3 / 22.8 | 156 | L71L (TTG→TTA) | ytfF | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (76/66); new base T (4/9); total (81/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 | |||||||||||
Only 100 of 168 total aligned reads displayed.
GCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAATGGGCAGC > minE/2880827‑2880964 | gcgcCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc > 1:2991855/1‑69 (MQ=255) ccAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGc > 1:5071882/1‑71 (MQ=255) ccAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGc > 1:4447230/1‑71 (MQ=255) gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc < 1:4999258/63‑1 (MQ=255) gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc < 1:2747728/63‑1 (MQ=255) gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc < 1:4414921/63‑1 (MQ=255) gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc < 1:682261/63‑1 (MQ=255) gTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATc < 1:3654813/63‑1 (MQ=255) cGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTaa > 1:4725206/1‑70 (MQ=255) cGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg > 1:1475554/1‑71 (MQ=255) cGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg > 1:3724160/1‑71 (MQ=255) cGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg > 1:4955831/1‑71 (MQ=255) cGTTGAATGGCACTGGCAAGGCAGAAGTAAAAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg > 1:2999366/1‑71 (MQ=255) gTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc < 1:4371471/71‑1 (MQ=255) tGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGc > 1:1875012/1‑69 (MQ=255) gAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAg > 1:5173878/1‑71 (MQ=255) gAATGGCACTGGCAAGGAAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCa > 1:4121131/1‑70 (MQ=255) gCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc > 1:3602458/1‑70 (MQ=255) gCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTcgc < 1:3981734/70‑1 (MQ=255) tGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTa < 1:4301696/56‑1 (MQ=255) tGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTa < 1:4633180/56‑1 (MQ=255) tGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTa < 1:3864597/56‑1 (MQ=255) tGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTa < 1:109793/56‑1 (MQ=255) tGGCAAGGCAGAAGTAATAGATGAGGTTGCCCACCATAGTGAGCATCAAGGCGGTa < 1:3120409/56‑1 (MQ=255) gCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg > 1:1231804/1‑56 (MQ=255) gCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg > 1:3450843/1‑56 (MQ=255) gCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg > 1:2609040/1‑56 (MQ=255) gCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAg > 1:2720337/1‑56 (MQ=255) gCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACgcgc > 1:1779278/1‑71 (MQ=255) aaGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCa < 1:4318205/71‑1 (MQ=255) aaGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCa < 1:5186876/71‑1 (MQ=255) ggCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAAc > 1:923530/1‑71 (MQ=255) gCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACt > 1:5022194/1‑71 (MQ=255) gCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACt < 1:502197/71‑1 (MQ=255) gCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACt > 1:2872604/1‑71 (MQ=255) gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa < 1:2310033/39‑1 (MQ=255) gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa < 1:832234/39‑1 (MQ=255) gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa < 1:1316368/39‑1 (MQ=255) gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa < 1:525441/39‑1 (MQ=255) gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa < 1:46228/39‑1 (MQ=255) gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa < 1:1927127/39‑1 (MQ=255) gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa < 1:2006335/39‑1 (MQ=255) gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa < 1:4764533/39‑1 (MQ=255) gAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCaa < 1:2503308/39‑1 (MQ=255) aGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAgg > 1:4496810/1‑39 (MQ=255) aGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgc < 1:2841148/71‑1 (MQ=255) aGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgc < 1:866256/71‑1 (MQ=255) aGTAATAGATGAGGTTGCCCATCATAGTGAGCATAAAGGCGGTAAGCCa > 1:4209417/1‑49 (MQ=255) gTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAAc > 1:624921/1‑64 (MQ=255) gTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAAc > 1:1382614/1‑64 (MQ=255) gTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca < 1:2362673/71‑1 (MQ=255) aGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgc < 1:1482138/65‑1 (MQ=255) ggTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGt > 1:2911510/1‑71 (MQ=255) ggTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGt > 1:4028079/1‑71 (MQ=255) gTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca < 1:4024587/59‑1 (MQ=255) gTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca < 1:4255674/59‑1 (MQ=255) gTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca < 1:356019/59‑1 (MQ=255) gTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca < 1:2598408/59‑1 (MQ=255) gTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca < 1:1858310/59‑1 (MQ=255) gTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacgca < 1:1137731/59‑1 (MQ=255) tGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTcc > 1:2872467/1‑70 (MQ=255) ccATCATAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTga < 1:384823/50‑1 (MQ=255) ccATCATAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTga < 1:1975150/50‑1 (MQ=255) ccATCATAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTga < 1:5167801/50‑1 (MQ=255) ccATCATAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTga < 1:710675/50‑1 (MQ=255) ccATCATAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTga < 1:1114267/50‑1 (MQ=255) ccATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagc > 1:5269/1‑71 (MQ=255) ccATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagc > 1:3405579/1‑71 (MQ=255) ccATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagc < 1:1805428/71‑1 (MQ=255) ccATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagc > 1:1112328/1‑71 (MQ=255) ccATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagc > 1:4455251/1‑71 (MQ=255) ccATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagc > 1:4882384/1‑71 (MQ=255) catcatAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagcc < 1:1016417/71‑1 (MQ=255) catcatAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagcc < 1:2835249/71‑1 (MQ=255) atcatAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacg > 1:2102664/1‑50 (MQ=255) atcatAGTGAGCATTAAGGCGGTAAGCCAGTCGCGACGCGCCAACTgacg > 1:1037512/1‑50 (MQ=255) catAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg > 1:753924/1‑71 (MQ=255) catAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg > 1:1747227/1‑71 (MQ=255) catAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg > 1:1875296/1‑71 (MQ=255) catAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg > 1:4564795/1‑71 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCaa > 1:3932611/1‑41 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg > 1:2680042/1‑70 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg > 1:4769014/1‑70 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg > 1:3526994/1‑70 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg > 1:2730042/1‑70 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAgg > 1:724673/1‑70 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGc < 1:4501516/71‑1 (MQ=255) atAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGc < 1:2095637/71‑1 (MQ=255) tAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGc > 1:314478/1‑70 (MQ=255) gTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAAt < 1:2437534/71‑1 (MQ=255) tGAGCATCTAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAAt < 1:309514/70‑1 (MQ=255) tGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAAt < 1:2528340/70‑1 (MQ=255) gAGCATCAAGGCGGCAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAAt < 1:2869330/69‑1 (MQ=255) aGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAATggg < 1:4059502/71‑1 (MQ=255) aGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAATggg < 1:3440046/71‑1 (MQ=255) gCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAATggg < 1:4984012/70‑1 (MQ=255) cATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCccagccag > 1:3347718/1‑61 (MQ=255) cATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCa < 1:4104329/64‑1 (MQ=255) aTCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCaa > 1:3628238/1‑64 (MQ=255) tCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAATGGGCAgc > 1:2151406/1‑71 (MQ=255) | GCGCCAGTACGTTGAATGGCACTGGCAAGGCAGAAGTAATAGATGAGGTTGCCCATCATAGTGAGCATCAAGGCGGTAAGCCAGTCGCGACGCGCCAACTGACGCAGACGCACGCGTCCCAGCCAGGCAATGGGCAGC > minE/2880827‑2880964 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |