| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,935,934 | A→G | 6.4% | intergenic (‑144/‑73) | idnD ← / → idnK | L‑idonate 5‑dehydrogenase, NAD‑binding/D‑gluconate kinase, thermosensitive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,935,934 | 0 | A | G | 6.4% | 379.3 / 11.0 | 125 | intergenic (‑144/‑73) | idnD/idnK | L‑idonate 5‑dehydrogenase, NAD‑binding/D‑gluconate kinase, thermosensitive |
| Reads supporting (aligned to +/- strand): ref base A (42/75); new base G (5/3); total (47/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 125 total aligned reads displayed.
GTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGG > minE/2935868‑2935997 | gTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAACTaa < 1:2558896/71‑1 (MQ=255)gTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaa < 1:3575580/71‑1 (MQ=255)gTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCATATCAGTAGAAATaa < 1:1247257/71‑1 (MQ=255) gAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaa < 1:2907910/71‑1 (MQ=255) gAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaa < 1:2048694/71‑1 (MQ=255) gAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaa < 1:1998987/71‑1 (MQ=255) gAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaa < 1:4151531/71‑1 (MQ=255) aaaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaaa > 1:2424979/1‑71 (MQ=255) aaaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaaa > 1:1154622/1‑71 (MQ=255) aaaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaaa > 1:1232203/1‑71 (MQ=255) aaaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaaa > 1:467614/1‑71 (MQ=255) aaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAAc > 1:1013219/1‑71 (MQ=255) aaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAAc > 1:2755272/1‑71 (MQ=255) aaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAAc > 1:4321911/1‑71 (MQ=255) aaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAAc > 1:5120419/1‑71 (MQ=255) aaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACg > 1:1521886/1‑71 (MQ=255) aaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACg < 1:2166439/71‑1 (MQ=255) gTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGa > 1:3140759/1‑70 (MQ=255) gTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGa > 1:2454559/1‑70 (MQ=255) gTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGa > 1:1385122/1‑70 (MQ=255) gTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGa < 1:1212506/70‑1 (MQ=255) gTTACAGTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGa > 1:671443/1‑70 (MQ=255) ttACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAGATAAAAAAACGTGaaa > 1:3381903/1‑71 (MQ=255) ttACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAGATAAAAAAACGTGaaa > 1:2775186/1‑71 (MQ=255) ttACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAGATAAAAAAACGTGaaa > 1:2826770/1‑71 (MQ=255) aTTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat < 1:4458163/71‑1 (MQ=255) ttACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattatt < 1:147104/71‑1 (MQ=255) cTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATtattat < 1:2746770/70‑1 (MQ=255) cTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATtattat < 1:2918105/70‑1 (MQ=255) cTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATtattat < 1:3591724/70‑1 (MQ=255) cTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATtattat < 1:2170862/70‑1 (MQ=255) cTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATg < 1:1050659/71‑1 (MQ=255) tGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATgccg > 1:3761154/1‑71 (MQ=255) gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatta < 1:3372482/62‑1 (MQ=255) gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatta < 1:1517914/62‑1 (MQ=255) gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:604743/60‑1 (MQ=255) gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:4243612/60‑1 (MQ=255) gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:4065974/60‑1 (MQ=255) gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:3181459/60‑1 (MQ=255) gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:4249105/60‑1 (MQ=255) gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:5145098/60‑1 (MQ=255) gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:1016608/60‑1 (MQ=255) gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:5126352/60‑1 (MQ=255) gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:2687033/60‑1 (MQ=255) gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:1972309/60‑1 (MQ=255) ttACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaa > 1:4008616/1‑49 (MQ=255) ttACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaa > 1:4206381/1‑49 (MQ=255) ttACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat < 1:2845615/62‑1 (MQ=255) aTAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgt < 1:3294772/71‑1 (MQ=255) tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAGATAAAAAAACGTGAAATTATTATGCCGCCAGGCgta < 1:1707430/71‑1 (MQ=255) tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAGATAAAAAAACGTGAAATTATTATGCCGCCAGGCgta < 1:138827/71‑1 (MQ=255) tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaa < 1:1033291/41‑1 (MQ=255) tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaa < 1:3755698/41‑1 (MQ=255) tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaa < 1:5077843/41‑1 (MQ=255) tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatt < 1:4806820/53‑1 (MQ=255) tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgt < 1:1348487/70‑1 (MQ=255) tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgt < 1:4717199/70‑1 (MQ=255) tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgt < 1:4737265/70‑1 (MQ=255) tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgt < 1:4239181/70‑1 (MQ=255) aaCGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatt < 1:4540138/52‑1 (MQ=255) aaCGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatt < 1:434041/52‑1 (MQ=255) aaCGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatt < 1:3682453/52‑1 (MQ=255) aaCGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatt < 1:402908/52‑1 (MQ=255) aCGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtag < 1:2716918/70‑1 (MQ=255) aCGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtag > 1:2675614/1‑70 (MQ=255) cGTGATGTGCCTTGTAATTTTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta > 1:4991813/1‑71 (MQ=255) cGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta > 1:4753611/1‑71 (MQ=255) cGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta > 1:2979143/1‑71 (MQ=255) cGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta > 1:265368/1‑71 (MQ=255) cGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta > 1:4941890/1‑71 (MQ=255) cGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta > 1:1080344/1‑71 (MQ=255) cGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta > 1:1279491/1‑71 (MQ=255) gtgCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCa > 1:2907536/1‑56 (MQ=255) gtgCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCa > 1:4529668/1‑56 (MQ=255) ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGaaa > 1:4584632/1‑39 (MQ=255) ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:4354548/40‑1 (MQ=255) ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:1366429/40‑1 (MQ=255) ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:1163407/40‑1 (MQ=255) ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:1119017/40‑1 (MQ=255) ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:678225/40‑1 (MQ=255) ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat < 1:1779378/40‑1 (MQ=255) ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGcagcag > 1:4064281/1‑71 (MQ=255) ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat < 1:1862693/41‑1 (MQ=255) ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat < 1:2780580/41‑1 (MQ=255) ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat < 1:87057/41‑1 (MQ=255) ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat < 1:2157384/41‑1 (MQ=255) ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat < 1:3673219/41‑1 (MQ=255) ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat < 1:1762335/41‑1 (MQ=255) ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat < 1:391511/41‑1 (MQ=255) ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATgc < 1:2569547/46‑1 (MQ=255) ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATgc < 1:214146/46‑1 (MQ=255) ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATgc < 1:647330/46‑1 (MQ=255) gTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATgccg > 1:1971149/1‑46 (MQ=255) cTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAgatga < 1:37523/70‑1 (MQ=255) ttATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTAt > 1:1571980/1‑52 (MQ=255) ttATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTAt > 1:928593/1‑52 (MQ=255) tCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAg < 1:969334/71‑1 (MQ=255) cAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCg < 1:352244/42‑1 (MQ=255) aGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAgg < 1:4852988/70‑1 (MQ=255) aGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAgg < 1:804493/70‑1 (MQ=255) | GTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGG > minE/2935868‑2935997 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |