| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,950,841 | T→C | 7.5% | L113S (TTA→TCA) | bglJ → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,950,841 | 0 | T | C | 7.5% | 274.7 / 9.7 | 93 | L113S (TTA→TCA) | bglJ | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (43/43); new base C (2/5); total (45/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
CGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAG > minE/2950775‑2950910 | cGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAAt < 1:3602326/71‑1 (MQ=255)cGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAAt < 1:2810455/71‑1 (MQ=255) gCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATg > 1:2092794/1‑71 (MQ=255) gCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATg > 1:468127/1‑71 (MQ=255) gCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATg > 1:4627822/1‑71 (MQ=255) gCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATg > 1:1670384/1‑71 (MQ=255) gCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATg > 1:2963357/1‑71 (MQ=255) gCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATg > 1:248060/1‑71 (MQ=255) gCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATg > 1:2518709/1‑71 (MQ=255) gtATTAAGTAAAGCGTCAACGCTGGAGATTTTTCCTCAGGAACTCTTTTTGTCATTAAATGGTGTACGt > 1:1712557/1‑69 (MQ=255) gtATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGt > 1:1885044/1‑69 (MQ=255) gtATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGt > 1:2839312/1‑69 (MQ=255) gtATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGt > 1:4046275/1‑69 (MQ=255) gtATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGt > 1:4836164/1‑69 (MQ=255) tATTAAGTAAAGCGTGAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAg > 1:4766073/1‑71 (MQ=255) tATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCa < 1:2700580/70‑1 (MQ=255) tATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAg > 1:27466/1‑71 (MQ=255) aTTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCa < 1:795633/69‑1 (MQ=255) ttAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTc < 1:4967505/67‑1 (MQ=255) ttAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTc < 1:4757856/67‑1 (MQ=255) tAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGgtg < 1:2946960/56‑1 (MQ=255) tAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGgtg < 1:1101097/56‑1 (MQ=255) tAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGgtg < 1:331970/56‑1 (MQ=255) tAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGgtg < 1:4083300/56‑1 (MQ=255) tAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGgtg < 1:3447889/56‑1 (MQ=255) aaaGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccga > 1:4235681/1‑71 (MQ=255) aaGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccgac > 1:2954197/1‑71 (MQ=255) aaGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccgac > 1:4133678/1‑71 (MQ=255) aGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATCAAATGgtg < 1:2705584/53‑1 (MQ=255) aGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATCAAATGgtg < 1:2475569/53‑1 (MQ=255) aGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATCAAATGgtg < 1:1198973/53‑1 (MQ=255) aGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATCAAATGgtg < 1:3756776/53‑1 (MQ=255) cGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATCAAATGGTGTa > 1:2244963/1‑53 (MQ=255) cGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATCAAATGGTGTa > 1:1907135/1‑53 (MQ=255) aaCGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAAt > 1:1344068/1‑43 (MQ=255) aaCGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTa > 1:1282864/1‑49 (MQ=255) aaCGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTa > 1:4025420/1‑49 (MQ=255) aaCGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACt > 1:2284267/1‑69 (MQ=255) aaCGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTg > 1:4093128/1‑70 (MQ=255) gCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACa < 1:1036311/71‑1 (MQ=255) gCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACa < 1:252139/71‑1 (MQ=255) gCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACa < 1:4651960/71‑1 (MQ=255) ggAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAAt > 1:4153648/1‑70 (MQ=255) gagaTTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAAt < 1:3771755/69‑1 (MQ=255) gagaTTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATc < 1:4541874/70‑1 (MQ=255) gagaTTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCa < 1:1621801/71‑1 (MQ=255) gaTTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAg > 1:4729551/1‑70 (MQ=255) gaTTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGt > 1:1680657/1‑71 (MQ=255) gaTTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGt > 1:2918648/1‑71 (MQ=255) ttttCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcga > 1:4885328/1‑47 (MQ=255) ttttCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACAGACCGACt > 1:1238059/1‑57 (MQ=255) atcaGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACAt > 1:603723/1‑70 (MQ=255) tcaGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTgg < 1:1793866/64‑1 (MQ=255) tcaGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTgg < 1:1312903/64‑1 (MQ=255) aGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCTACCGACTGAACAATCAGTg < 1:1814883/61‑1 (MQ=255) aGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTg < 1:2371370/61‑1 (MQ=255) aGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTg < 1:1553864/61‑1 (MQ=255) aGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTg < 1:1858290/61‑1 (MQ=255) aGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTg < 1:3316307/61‑1 (MQ=255) aGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTg < 1:3729746/61‑1 (MQ=255) aGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTaa < 1:2887636/70‑1 (MQ=255) aGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAAc > 1:1720923/1‑71 (MQ=255) gAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGt > 1:2948248/1‑61 (MQ=255) gAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGt > 1:1278455/1‑61 (MQ=255) gAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGt > 1:2724928/1‑61 (MQ=255) gAACTCTTTTTGTCATTAAATGGGGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGt > 1:4757202/1‑61 (MQ=255) aCTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCaaa < 1:4124538/71‑1 (MQ=255) ctctTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAAcc < 1:2336434/67‑1 (MQ=255) ctTTTTGTCATTAAATGGTGTACGTCAGGcgaccgaccg < 1:4988910/39‑1 (MQ=255) tttttGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCg > 1:4152439/1‑71 (MQ=255) tttGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCa > 1:1910858/1‑48 (MQ=255) tttGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCgg < 1:4268024/70‑1 (MQ=255) tttGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCgg < 1:3941708/70‑1 (MQ=255) tttGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCgg < 1:2443588/70‑1 (MQ=255) tCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCgg < 1:4131597/66‑1 (MQ=255) tCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCgg < 1:2037079/66‑1 (MQ=255) tCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCgg < 1:2292568/66‑1 (MQ=255) tCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCgg < 1:75241/66‑1 (MQ=255) tCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCgg < 1:3166605/66‑1 (MQ=255) tCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGtt < 1:1080822/71‑1 (MQ=255) aTTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGAc > 1:2138498/1‑66 (MQ=255) aTTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGAc > 1:2685079/1‑66 (MQ=255) aTTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGAc > 1:1128400/1‑66 (MQ=255) aTTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGAc > 1:1920912/1‑66 (MQ=255) aTTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTa < 1:1765726/70‑1 (MQ=255) aTTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTa < 1:2833077/70‑1 (MQ=255) aTTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTa < 1:1369486/70‑1 (MQ=255) aTTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTa < 1:3012722/70‑1 (MQ=255) aTTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTa < 1:3199086/70‑1 (MQ=255) ttAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATc < 1:1904640/40‑1 (MQ=255) ttAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATc < 1:573567/40‑1 (MQ=255) ttAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATc < 1:4806688/40‑1 (MQ=255) ttAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATc < 1:2915199/40‑1 (MQ=255) ttAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATc < 1:3416280/40‑1 (MQ=255) ttAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATc < 1:2130092/40‑1 (MQ=255) ttAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATc < 1:2460446/40‑1 (MQ=255) ttAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAg > 1:704155/1‑71 (MQ=255) ttAAATGGTGTACGGCAGGCGACCGACCGACTGAACAATc < 1:1110379/40‑1 (MQ=255) tAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTa > 1:3104496/1‑46 (MQ=255) tAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTa > 1:2737633/1‑46 (MQ=255) | CGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAG > minE/2950775‑2950910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |