Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,965,083	G→T	10.0%	A189S (GCG→TCG) ‡	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,965,083	0	G	T	10.0%	223.7 / 7.0	69	A189S (GCG→TCG) ‡	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (20/42);  new base T (4/3);  total (24/45)
Fisher's exact test for biased strand distribution p-value = 2.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

TTTCTACGCGACCCGTGATATTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATG  >  minE/2965016‑2965151                                                                    |                                                                     tttCTACGCGACCCGTGATATTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCg                                                                    <  1:1218432/70‑1 (MQ=255) tttCTACGCGACCCGTGATATTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCg                                                                    <  1:452445/70‑1 (MQ=255)   tCTACGCGACCCGTGATATTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaa                                                                  <  1:2186723/70‑1 (MQ=255)                     ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                                <  1:2363939/54‑1 (MQ=255)                     ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                                <  1:3529885/54‑1 (MQ=255)                     ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                                <  1:1183175/54‑1 (MQ=255)                     ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                                <  1:3113423/54‑1 (MQ=255)                     ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                                <  1:3936596/54‑1 (MQ=255)                     ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                                <  1:2639714/54‑1 (MQ=255)                     ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                                <  1:4580373/54‑1 (MQ=255)                     ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCgaaga                                                                <  1:176713/54‑1 (MQ=255)                     ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                               <  1:4556448/71‑1 (MQ=255)                     ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                               <  1:2187430/71‑1 (MQ=255)                     ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                               <  1:172204/71‑1 (MQ=255)                     ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                               <  1:4275318/71‑1 (MQ=255)                     ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                               <  1:3981556/71‑1 (MQ=255)                       aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                              <  1:346309/70‑1 (MQ=255)                       aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                              <  1:3606100/70‑1 (MQ=255)                       aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                              <  1:5040416/70‑1 (MQ=255)                        ccGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAc                                            >  1:4813304/1‑71 (MQ=255)                           cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                         >  1:248198/1‑71 (MQ=255)                           cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                         >  1:3720867/1‑71 (MQ=255)                           cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                         >  1:4779475/1‑71 (MQ=255)                           cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgc                                          >  1:3699205/1‑70 (MQ=255)                           cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcg                                           >  1:5087612/1‑69 (MQ=255)                             aCCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCt                                       <  1:2012054/71‑1 (MQ=255)                                 tgtaCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgc                                          <  1:4562786/61‑1 (MQ=255)                                 tGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGtt                                   <  1:2211737/71‑2 (MQ=255)                                 tGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTg                                   <  1:1541262/71‑1 (MQ=255)                                    aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                <  1:4317285/71‑1 (MQ=255)                                    aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                <  1:3819659/71‑1 (MQ=255)                                    aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                <  1:3661095/71‑1 (MQ=255)                                    aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                <  1:1747669/71‑1 (MQ=255)                                    aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                <  1:2133124/71‑1 (MQ=255)                                        cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGAc                             >  1:726518/1‑70 (MQ=255)                                        cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACg                            >  1:2423087/1‑71 (MQ=255)                                        cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGCAAGGTCTGGa                                             >  1:4767651/1‑54 (MQ=255)                                            ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                         <  1:933279/70‑1 (MQ=255)                                            ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                         <  1:2422454/70‑1 (MQ=255)                                            ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                         <  1:748009/70‑1 (MQ=255)                                            ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                         <  1:749094/70‑1 (MQ=255)                                                tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                    >  1:627910/1‑70 (MQ=255)                                                tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                    >  1:5139499/1‑70 (MQ=255)                                                tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                    >  1:4654633/1‑70 (MQ=255)                                                tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                    >  1:3765659/1‑70 (MQ=255)                                                 gATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTgg                   >  1:2511946/1‑71 (MQ=255)                                                    cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                     >  1:542705/1‑50 (MQ=255)                                                    cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                     >  1:509576/1‑50 (MQ=255)                                                    cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                     >  1:5088838/1‑50 (MQ=255)                                                      ccGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgc              <  1:1564036/71‑1 (MQ=255)                                                      ccGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgc              <  1:1572932/71‑1 (MQ=255)                                                       cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg             <  1:4421560/68‑1 (MQ=255)                                                       cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg             <  1:5096278/68‑1 (MQ=255)                                                       cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg             <  1:5072468/68‑1 (MQ=255)                                                         ccTCTATTCTGGCGAAGAAACTAGCGGAAGGACTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgcggc           >  1:1677587/1‑71 (MQ=255)                                                           tctATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                       >  1:5198074/1‑57 (MQ=255)                                                             tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                    <  1:1698747/42‑1 (MQ=255)                                                             tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                    <  1:3360109/42‑1 (MQ=255)                                                             tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                    <  1:1788533/42‑1 (MQ=255)                                                             tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                    <  1:3353852/42‑1 (MQ=255)                                                             tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGt       >  1:3431812/1‑71 (MQ=255)                                                             tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGt       >  1:3394182/1‑71 (MQ=255)                                                               ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                              <  1:4703971/46‑1 (MQ=255)                                                               ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                              <  1:2363989/46‑1 (MQ=255)                                                               ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                              <  1:3111659/46‑1 (MQ=255)                                                               ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGtt      <  1:4676491/70‑1 (MQ=255)                                                                tCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                     >  1:752776/1‑38 (MQ=255)                                                                tCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                     >  1:3567664/1‑38 (MQ=255)                                                                 cTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa    >  1:3790149/1‑70 (MQ=255)                                                                    gCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTAt   <  1:288183/68‑1 (MQ=255)                                                                    gCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATg  <  1:3369350/69‑1 (MQ=255)                                                                    |                                                                     TTTCTACGCGACCCGTGATATTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATG  >  minE/2965016‑2965151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑