| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 187,851 | G→A | 6.0% | A136T (GCG→ACG) | accA → | acetylCoA carboxylase, carboxytransferase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 187,851 | 0 | G | A | 6.0% | 205.1 / 2.7 | 66 | A136T (GCG→ACG) | accA | acetylCoA carboxylase, carboxytransferase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (29/33); new base A (2/2); total (31/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
TGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGAT > minE/187783‑187909 | tgatgaTCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAgcg > 1:4548250/1‑71 (MQ=255) gatgatCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAgcgc > 1:2659915/1‑71 (MQ=255) tgatCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAgcg > 1:4411812/1‑68 (MQ=255) atCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAg < 1:3061953/70‑1 (MQ=255) atCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAg < 1:3738435/70‑1 (MQ=255) tCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAg > 1:4250914/1‑69 (MQ=255) tCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAg > 1:449033/1‑69 (MQ=255) tCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAg > 1:2545481/1‑69 (MQ=255) tCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAg > 1:3245823/1‑69 (MQ=255) tCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAg > 1:5136706/1‑69 (MQ=255) tCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAg > 1:3874589/1‑69 (MQ=255) cATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAg > 1:3375372/1‑68 (MQ=255) cATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAg > 1:3574289/1‑71 (MQ=255) atcaAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAgcgc < 1:1893757/57‑1 (MQ=255) atcaAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAgcgc < 1:792904/57‑1 (MQ=255) atcaAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAgcgc < 1:1196606/57‑1 (MQ=255) atcaAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAgcgc < 1:4548743/57‑1 (MQ=255) atcaAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAgcgc < 1:1209312/57‑1 (MQ=255) tcaAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTa < 1:4091674/66‑1 (MQ=255) tcaAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCa < 1:1315546/71‑1 (MQ=255) tcaAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCa < 1:3319518/71‑1 (MQ=255) tcaAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCa < 1:2180886/71‑1 (MQ=255) aaaaaGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAg > 1:140825/1‑57 (MQ=255) aaaaaGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGc > 1:4037559/1‑68 (MQ=255) aaaaaGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCaa < 1:3000009/70‑1 (MQ=255) aaGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAgcgc < 1:518170/51‑1 (MQ=255) aGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAgcg > 1:3612459/1‑49 (MQ=255) aGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAgcg > 1:1150639/1‑49 (MQ=255) tCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGc < 1:4127387/66‑1 (MQ=255) tCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGc < 1:901508/66‑1 (MQ=255) tCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGc < 1:1250590/66‑1 (MQ=255) tCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGc < 1:3763868/66‑1 (MQ=255) tCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGc < 1:226447/71‑1 (MQ=255) tCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGc < 1:2994626/71‑1 (MQ=255) tCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGc < 1:3646867/71‑1 (MQ=255) cGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCg > 1:4463177/1‑59 (MQ=255) cGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCg > 1:4280256/1‑59 (MQ=255) gTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGt < 1:4542102/71‑1 (MQ=255) gTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGt < 1:3023350/71‑1 (MQ=255) gTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGt < 1:62089/71‑1 (MQ=255) aaaCCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGa < 1:385884/46‑1 (MQ=255) aaaCCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGa < 1:1865887/46‑1 (MQ=255) aaaCCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAACGCCAg < 1:89519/45‑1 (MQ=255) aaaCCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAACGCCAg < 1:4685899/45‑1 (MQ=255) aCCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAACGCCAGaa > 1:3868678/1‑45 (MQ=255) aCCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAACGCCAGaa > 1:3377324/1‑45 (MQ=255) aaaGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGt < 1:1292282/63‑1 (MQ=255) aaaGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCa < 1:3968062/71‑1 (MQ=255) aGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCAc > 1:4143565/1‑56 (MQ=255) aGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCaaa < 1:838484/71‑1 (MQ=255) gAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCg < 1:2779077/59‑1 (MQ=255) aaaaaaTTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATg > 1:1650341/1‑71 (MQ=255) aaaaTTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGc > 1:949362/1‑71 (MQ=255) aaaaTTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGc > 1:421102/1‑71 (MQ=255) aaaaTTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGc > 1:1977297/1‑71 (MQ=255) aaaTTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCt > 1:4917518/1‑71 (MQ=255) aaaTTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCt > 1:4868056/1‑71 (MQ=255) aaTTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTg < 1:4644875/71‑1 (MQ=255) tAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCa < 1:473018/40‑1 (MQ=255) tAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCa < 1:1153744/40‑1 (MQ=255) tAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCttt > 1:333147/1‑70 (MQ=255) aaCTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCa > 1:1858265/1‑39 (MQ=255) aaCTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCa > 1:2754090/1‑39 (MQ=255) aaCTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCa > 1:2241199/1‑39 (MQ=255) aCTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCttt > 1:2011957/1‑68 (MQ=255) aCTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCttt > 1:670208/1‑68 (MQ=255) aCTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCttt > 1:641780/1‑68 (MQ=255) aCTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCtt > 1:2383940/1‑67 (MQ=255) aCTTTCGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCttt > 1:5098842/1‑68 (MQ=255) tttGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTaa < 1:1110170/68‑1 (MQ=255) tttGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTaa < 1:2906656/68‑1 (MQ=255) tttGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTaa < 1:2930361/68‑1 (MQ=255) tttGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTaa < 1:3457722/68‑1 (MQ=255) tttGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTaa < 1:3501623/68‑1 (MQ=255) tttGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGAt > 1:2896742/1‑71 (MQ=255) tGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAAt < 1:2261116/46‑1 (MQ=255) tGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAAt < 1:2298500/46‑1 (MQ=255) | TGATGATCATTGGTCATCAAAAAGGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTACCGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGAT > minE/187783‑187909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |