| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 217,996 | A→T | 18.5% | intergenic (‑88/‑18) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 217,996 | 0 | A | T | 18.5% | 128.6 / 26.1 | 60 | intergenic (‑88/‑18) | yafU/yafF | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (30/18); new base T (7/4); total (37/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01 | |||||||||||
CTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTT > minE/217928‑218065 | ctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAgg > 1:2937070/1‑71 (MQ=255) tgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGc > 1:3919022/1‑71 (MQ=255) tgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGc > 1:2751679/1‑71 (MQ=255) aTTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGt < 1:3954901/71‑1 (MQ=255) cGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACg < 1:2151764/71‑1 (MQ=255) ttCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa > 1:3326733/1‑71 (MQ=255) ttCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa > 1:975920/1‑71 (MQ=255) ttCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa > 1:907351/1‑71 (MQ=255) ttCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa > 1:4029888/1‑71 (MQ=255) ttCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa > 1:3499276/1‑71 (MQ=255) ttCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGGaatgaa > 1:3503472/1‑71 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatgaat > 1:4132721/1‑70 (MQ=255) ccACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatga < 1:3809680/66‑1 (MQ=255) caGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGc > 1:1450720/1‑45 (MQ=255) aGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:2076933/49‑1 (MQ=255) aGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:2109642/49‑1 (MQ=255) aGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:3250859/49‑1 (MQ=255) aGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:43725/49‑1 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACCGGCGTCTGGACGTGGTAATGAATGAAGACGACTAc < 1:1041421/71‑1 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTAc > 1:1504268/1‑70 (MQ=255) cGAAACCACTGGCACGTGGAGAATAAGCTTCACAGGCGTCTGGACGTgg > 1:883595/1‑49 (MQ=255) cGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTgg > 1:1376534/1‑49 (MQ=255) cGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTgg > 1:73805/1‑49 (MQ=255) cACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAAt > 1:541836/1‑68 (MQ=255) cTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATaagaa < 1:1397145/71‑1 (MQ=255) ggCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGt > 1:5087936/1‑37 (MQ=255) ggCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGt > 1:4980495/1‑37 (MQ=255) ggCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGt > 1:2602345/1‑37 (MQ=255) ggCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGt > 1:3158220/1‑37 (MQ=255) ggCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGt > 1:1258041/1‑37 (MQ=255) ggCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGt > 1:791585/1‑37 (MQ=255) ggCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGt > 1:1470093/1‑37 (MQ=255) ggCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGt > 1:3970293/1‑37 (MQ=255) gCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAagag > 1:4987817/1‑71 (MQ=255) aCGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGa < 1:5194263/71‑1 (MQ=255) cGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACa < 1:2859138/56‑1 (MQ=255) cGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGa < 1:1057529/70‑1 (MQ=255) tGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaa > 1:3890336/1‑36 (MQ=255) tGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGaaa > 1:250428/1‑70 (MQ=255) tGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGaaa > 1:233039/1‑70 (MQ=255) tGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAAc < 1:1305037/71‑1 (MQ=255) tGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAAc < 1:3019170/71‑1 (MQ=255) agaATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:2092532/35‑1 (MQ=255) agaATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:146182/35‑1 (MQ=255) agaATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:567016/35‑1 (MQ=255) gaATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:2305783/1‑71 (MQ=255) aaTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGa < 1:1058646/63‑1 (MQ=255) aaTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:955827/1‑70 (MQ=255) aTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGaa < 1:2866950/63‑1 (MQ=255) aTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGa < 1:391354/62‑1 (MQ=255) aTAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGa < 1:444470/62‑1 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGAGGACTACAAAATAAGAAGAGGAAACgcag > 1:1385863/1‑68 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGaaa > 1:2072342/1‑63 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAAc > 1:4552892/1‑64 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:2820525/1‑68 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:314078/1‑68 (MQ=255) tAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACgcag > 1:97438/1‑68 (MQ=255) aGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGaa > 1:3219740/1‑71 (MQ=255) cTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAatt < 1:506999/71‑1 (MQ=255) cTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAatt < 1:4167812/71‑1 (MQ=255) cacaGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAatta < 1:4206903/69‑1 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:52085/1‑70 (MQ=255) aGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTAtttt > 1:8537/1‑70 (MQ=255) | CTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTT > minE/217928‑218065 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |