| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 373,682 | G→T | 6.3% | A178S (GCG→TCG) | fepE → | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 373,682 | 0 | G | T | 6.3% | 317.5 / 4.9 | 94 | A178S (GCG→TCG) | fepE | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Reads supporting (aligned to +/- strand): ref base G (53/35); new base T (3/3); total (56/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
ACAATGCCAGTAAGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTATATCTCTACGTTGGTGGTGAA > minE/373620‑373752 | aCAATGCCAGTAAGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAAc < 1:4379779/70‑1 (MQ=255) aaTGCCAGTAAGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAg > 1:938849/1‑71 (MQ=255) aaTGCCAGTAAGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAg > 1:3612599/1‑71 (MQ=255) aTGCCAGTAAGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGt > 1:1596492/1‑71 (MQ=255) ccAGTAAGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGaa < 1:2342775/71‑1 (MQ=255) tAAGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGcc < 1:3404709/57‑1 (MQ=255) tAAGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGcc < 1:2240228/57‑1 (MQ=255) tAAGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGa < 1:1760517/66‑1 (MQ=255) tAAGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGa < 1:1437887/66‑1 (MQ=255) aGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAag > 1:109609/1‑66 (MQ=255) aGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAag > 1:1772854/1‑66 (MQ=255) aGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGgcg > 1:4644084/1‑71 (MQ=255) aGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGgcg > 1:1576524/1‑71 (MQ=255) aGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGgcg > 1:3731025/1‑71 (MQ=255) aGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGgcg > 1:58897/1‑71 (MQ=255) gAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGgcg > 1:3322123/1‑70 (MQ=255) aaaaaGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCa < 1:3661420/70‑1 (MQ=255) gATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGt < 1:1449248/71‑1 (MQ=255) gATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGt < 1:1543279/71‑1 (MQ=255) gATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGt < 1:4378999/71‑1 (MQ=255) gATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGt < 1:640976/71‑1 (MQ=255) tGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAAcc < 1:2569378/49‑1 (MQ=255) aaCCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTg < 1:2020203/71‑1 (MQ=255) aCCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTg < 1:3133432/70‑1 (MQ=255) aCCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTg < 1:3546437/70‑1 (MQ=255) aCCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGa < 1:1369877/71‑1 (MQ=255) ccGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAg < 1:3297352/71‑1 (MQ=255) ccGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAg < 1:707873/71‑1 (MQ=255) ccGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAg < 1:615491/71‑1 (MQ=255) tCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGCCCGTTTTGAGCgg > 1:565770/1‑71 (MQ=255) tCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGa > 1:2481968/1‑67 (MQ=255) tCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCgg > 1:4286484/1‑71 (MQ=255) tCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCgg > 1:20444/1‑71 (MQ=255) cTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGgcg < 1:1230508/51‑1 (MQ=255) cTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGgcg < 1:904526/51‑1 (MQ=255) cTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGgcg < 1:1033828/51‑1 (MQ=255) cTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGgcg < 1:2453952/51‑1 (MQ=255) cTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGgcg < 1:3643662/51‑1 (MQ=255) tGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGaa > 1:3829657/1‑44 (MQ=255) tGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGaa > 1:988523/1‑44 (MQ=255) tGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGaa > 1:3569533/1‑44 (MQ=255) tGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGaa > 1:2578221/1‑44 (MQ=255) tGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGaa > 1:15024/1‑44 (MQ=255) tGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGaa > 1:2500364/1‑44 (MQ=255) tGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGaa > 1:1184084/1‑44 (MQ=255) tataCCTCCTGGACGCTAAGTTTTACCTCGCCAACCAGTGAAGAGGCGCAGACCGt > 1:4000525/1‑56 (MQ=255) tataCCTCCTGGACGCTAAGTTTTACCTCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTatat > 1:1594792/1‑71 (MQ=255) tataCCTCCTGGACGCTAAGTTTTACCTCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTatat > 1:5044160/1‑71 (MQ=255) tataCCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTatat > 1:594630/1‑71 (MQ=255) taCCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAaga > 1:2313734/1‑42 (MQ=255) taCCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAaga > 1:4245293/1‑42 (MQ=255) taCCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAaga > 1:4374766/1‑42 (MQ=255) taCCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAaga > 1:4408883/1‑42 (MQ=255) taCCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAaga > 1:3576534/1‑42 (MQ=255) taCCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAaga > 1:2248681/1‑42 (MQ=255) taCCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAaga > 1:5043498/1‑42 (MQ=255) taCCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAaga > 1:1048385/1‑42 (MQ=255) taCCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAag > 1:3479299/1‑41 (MQ=255) cctcctGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGAt > 1:5025839/1‑71 (MQ=255) cctcctGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGAt > 1:1341546/1‑71 (MQ=255) cctcctGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGAt > 1:1199031/1‑71 (MQ=255) cctcctGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGAt > 1:1918316/1‑71 (MQ=255) cctcctGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGAt > 1:3095979/1‑71 (MQ=255) cctcctGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGAt > 1:3900618/1‑71 (MQ=255) ctcctGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGAt > 1:107499/1‑70 (MQ=255) tcctGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGAt < 1:4115847/69‑1 (MQ=255) tcctGGACGCTAAGTTTTACCGCGCCAACCAATGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGAt < 1:2500763/69‑1 (MQ=255) ctGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATtata > 1:1025875/1‑71 (MQ=255) ctGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATtata > 1:1133023/1‑71 (MQ=255) ctGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATtata > 1:2849340/1‑71 (MQ=255) ctGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATtat < 1:4786096/70‑1 (MQ=255) ctGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATtat < 1:3627810/70‑1 (MQ=255) gACGCTAAGTTTTACCTCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTatat < 1:4844735/69‑1 (MQ=255) gACGCTAAGTTTTACCTCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTatat < 1:2164970/69‑1 (MQ=255) gACGCTAAGTTTTACCTCGCCAACCAATGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTatat < 1:1564589/69‑1 (MQ=255) gACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTatat > 1:3036361/1‑69 (MQ=255) gACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTATAtct > 1:1599833/1‑71 (MQ=255) gACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTATAtc > 1:5191253/1‑70 (MQ=255) cGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGtt < 1:4330211/44‑1 (MQ=255) cGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGtt < 1:5170908/44‑1 (MQ=255) aGTTTTACCGCGCCAACCGGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTatat > 1:4688263/1‑62 (MQ=255) aGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCTTTTTGAGCGGGTATATCGATTATATCTCTACGtt < 1:3314932/71‑1 (MQ=255) aGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTatat > 1:3295924/1‑62 (MQ=255) aGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTatat > 1:4291392/1‑62 (MQ=255) aGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTatat > 1:133636/1‑62 (MQ=255) aGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTATATCTCTACGtt < 1:1799457/71‑1 (MQ=255) gTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTATATCTCTACGtt < 1:2363098/70‑1 (MQ=255) ttttACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTATATCTCTACGTtgg < 1:3406397/71‑1 (MQ=255) ttAGCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGAt < 1:2026365/53‑1 (MQ=255) ttACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGa > 1:3565523/1‑52 (MQ=255) ttACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGa > 1:4944753/1‑52 (MQ=255) ttACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGa > 1:1167888/1‑52 (MQ=255) ttACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGa > 1:3630533/1‑52 (MQ=255) ttACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGa > 1:3034865/1‑52 (MQ=255) cgcgCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTATATCTCTACGTTGGTGGTGa < 1:4947270/71‑1 (MQ=255) cgcgCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTATATCTCTACGTTGGTGGTGa < 1:3382250/71‑1 (MQ=255) gcgcCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTATATCTCTACGTTGGTGGTGaa < 1:2392641/71‑1 (MQ=255) gcgcCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTATATCTCTACGTTGGTGGTGaa < 1:4381882/71‑1 (MQ=255) | ACAATGCCAGTAAGAAAAAAGATGAACCGTCACTGTATACCTCCTGGACGCTAAGTTTTACCGCGCCAACCAGTGAAGAGGCGCAGACCGTTTTGAGCGGGTATATCGATTATATCTCTACGTTGGTGGTGAA > minE/373620‑373752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |