| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 385,816 | T→C | 7.0% | H342H (CAT→CAC) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 385,816 | 0 | T | C | 7.0% | 252.4 / 7.4 | 86 | H342H (CAT→CAC) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base T (32/48); new base C (2/4); total (34/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01 | |||||||||||
CGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTAC > minE/385746‑385885 | cGGTATGGACCGGTAACCTGTTCCCGTTCATGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCAt > 1:4729444/1‑71 (MQ=255) ggTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:4664225/71‑1 (MQ=255) aTGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcgc > 1:3233171/1‑71 (MQ=255) aTGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcgc > 1:5124392/1‑71 (MQ=255) aTGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcgc > 1:338770/1‑71 (MQ=255) aTGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcgc > 1:4593394/1‑71 (MQ=255) aTGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcgc > 1:2293883/1‑71 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:1916734/67‑1 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:3573833/67‑1 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:2172157/67‑1 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:1708200/67‑1 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:3341666/67‑1 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:3253962/67‑1 (MQ=255) tGGACCGGTAACATGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:2504617/67‑1 (MQ=255) ggACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:5199712/66‑1 (MQ=255) gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg > 1:3877731/1‑67 (MQ=255) gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg < 1:3259093/70‑1 (MQ=255) aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGa > 1:2782091/1‑70 (MQ=255) aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt > 1:279950/1‑71 (MQ=255) aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt > 1:549291/1‑71 (MQ=255) aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt > 1:2420182/1‑71 (MQ=255) ggTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtctc < 1:2254526/71‑1 (MQ=255) gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtctc < 1:647515/70‑1 (MQ=255) gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct > 1:1815246/1‑71 (MQ=255) gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct > 1:4263547/1‑71 (MQ=255) gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct > 1:4124511/1‑71 (MQ=255) cttCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCACGCGCTGATCTCGTCGGGCACCACGc > 1:2451785/2‑71 (MQ=255) cttCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCACGCGCTGATCTCGTCGGGCACCACGc > 1:4431645/2‑71 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg < 1:4726845/48‑1 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg < 1:382769/48‑1 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg < 1:2896013/48‑1 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg < 1:679302/48‑1 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg < 1:4589741/48‑1 (MQ=255) ccTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCg > 1:3400471/1‑70 (MQ=255) ccTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCg > 1:3810118/1‑70 (MQ=255) ccTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCACGCGCTGAt < 1:3124818/51‑1 (MQ=19) ccTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCACGCGCTGAt < 1:5014864/51‑1 (MQ=19) ccTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCACGCGCTGAt < 1:1897151/51‑1 (MQ=19) tGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCg > 1:4142249/1‑68 (MQ=255) tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg < 1:3288923/56‑1 (MQ=255) tATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg > 1:1055302/1‑54 (MQ=255) tATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGa > 1:1016631/1‑56 (MQ=255) tATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGa > 1:1204589/1‑56 (MQ=255) tATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGa > 1:2066375/1‑56 (MQ=255) cACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTgg > 1:1807834/1‑71 (MQ=255) aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc > 1:3757847/1‑71 (MQ=255) aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc > 1:2577401/1‑71 (MQ=255) ccATCGCCTGCGGCGCCGTCTCGGGCTTCCACGCGCTGATCTCGTCGGGCACCACGCCCAAGATGCtcg < 1:754585/69‑3 (MQ=255) cATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg < 1:519833/37‑1 (MQ=255) tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac > 1:4112289/1‑71 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:3601303/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:3289528/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:4240537/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:4238302/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:5012806/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:390373/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg > 1:3509276/1‑70 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:5030359/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:2187567/70‑1 (MQ=255) ggTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGt > 1:1695415/1‑71 (MQ=255) gtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCt > 1:1330233/1‑71 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGaa < 1:982607/41‑1 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGaa < 1:3567546/41‑1 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGaa < 1:3547780/41‑1 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGaa < 1:3415445/41‑1 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGaa < 1:23700/41‑1 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGaa < 1:2073813/41‑1 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGc < 1:4076970/61‑1 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGc < 1:914255/61‑1 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGc < 1:4973737/61‑1 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGc < 1:1735831/61‑1 (MQ=255) tCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCGGGCGAACGAAGGGc < 1:717418/61‑1 (MQ=255) gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTAt > 1:5191099/1‑70 (MQ=255) gCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTAt > 1:1123056/1‑70 (MQ=255) tCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:156570/49‑1 (MQ=255) tCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:537286/49‑1 (MQ=255) tCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:1180339/49‑1 (MQ=255) tCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:116797/49‑1 (MQ=255) tCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:494122/49‑1 (MQ=255) tCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:722011/49‑1 (MQ=255) tCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:3974681/49‑1 (MQ=255) ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacga < 1:3146232/48‑1 (MQ=255) ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGc > 1:4794289/1‑70 (MQ=255) ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGc > 1:1099885/1‑70 (MQ=255) ccATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAAAGAAGGGCAGGCGTGCTTTATCGGc > 1:647684/1‑70 (MQ=255) aTGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTAc < 1:4485932/71‑1 (MQ=255) aTGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTAc < 1:1527584/71‑1 (MQ=255) | CGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCTTTATCGGCTAC > minE/385746‑385885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |